The immunohistochemical evaluation of the expression of intermediary filaments, pcna, p53 and mmp-9 in feline fibrosarcomas
<b>Aim: </b>The present study was aimed at the immunohistochemical evaluation of the expression of cancer indicators; including the Proliferating Cell Nucleus Antigen (PCNA), Matrix Metalloproteinase-9 (MMP-9), vimentin, alphasmooth muscle actin (?-SMA), S-100, desmin and pan-Cytokeratin...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1423 |
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| Summary: | <b>Aim: </b>The present study was aimed at the immunohistochemical evaluation of
the expression of cancer indicators; including the Proliferating Cell Nucleus
Antigen (PCNA), Matrix Metalloproteinase-9 (MMP-9), vimentin, alphasmooth
muscle actin (?-SMA), S-100, desmin and pan-Cytokeratin (Pan-
CK), as well as intermediary filaments, with a view to demonstrate the cell
proliferation index, p53 tumour-suppressor gene status, tumour metastasis,
invasion capacity and cellular origin of feline fibrosarcomas.<p>
<b>Materials and Methods: </b>The study material comprised of tumoral tissue
samples from 7 cats, which were referred to the Pathology Department
for routine histopathological examination. In order to evaluate the
histopathological changes, the sections were stained with Haematoxylin
and Eosin. Streptavidin-biotin peroxidase complex method was used for
immunohistochemical staining.<p>
<b>Results: </b>Out of the seven spontaneous cutaneous fibrosarcoma cases, five
were in stage I, one was in stage II, and one was in stage III. The presence
of conspicuous collagen bands in the tumoral tissues was demonstrated by
Masson's trichrome (MT) staining. All cases stained positively for vimentin and
?-SMA. The tumoral cells reacted negatively for pan-CK, S-100 and desmin. All
cases were immunopositive for PCNA. Two cases stained positively for p53.
These two cases were advanced stage. Only a single case of fibrosarcoma
(stage III) presented with intracytoplasmic MMP-9 expression in neoplastic
giant cells.<p>
<b>Conclusion:</b> Despite only very few tumour samples having been examined
in the present study, it was concluded that PCNA, p53 and MMP-9 served as
highly useful indicators for determining cell proliferation, grading malignancy,
detecting tumour aggressiveness and behaviour, as well as predicting
prognosis in spontaneous feline fibrosarcomas. |
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| ISSN: | 1309-6958 2146-1953 |