Enhanced antibody responses in CD19-Cre mice
Abstract CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mic...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83954-8 |
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| author | Diogo M. Cunha Sara Hernández-Pérez Pieta K. Mattila |
| author_facet | Diogo M. Cunha Sara Hernández-Pérez Pieta K. Mattila |
| author_sort | Diogo M. Cunha |
| collection | DOAJ |
| description | Abstract CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research. |
| format | Article |
| id | doaj-art-acfaad71ed4a49dba6224522bcbb64df |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-acfaad71ed4a49dba6224522bcbb64df2025-01-12T12:14:44ZengNature PortfolioScientific Reports2045-23222025-01-011511810.1038/s41598-024-83954-8Enhanced antibody responses in CD19-Cre miceDiogo M. Cunha0Sara Hernández-Pérez1Pieta K. Mattila2Institute of Biomedicine and MediCity Research Laboratories, University of TurkuInstitute of Biomedicine and MediCity Research Laboratories, University of TurkuInstitute of Biomedicine and MediCity Research Laboratories, University of TurkuAbstract CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.https://doi.org/10.1038/s41598-024-83954-8B cellsBCRCD19Antibody responseClass-switch recombination |
| spellingShingle | Diogo M. Cunha Sara Hernández-Pérez Pieta K. Mattila Enhanced antibody responses in CD19-Cre mice Scientific Reports B cells BCR CD19 Antibody response Class-switch recombination |
| title | Enhanced antibody responses in CD19-Cre mice |
| title_full | Enhanced antibody responses in CD19-Cre mice |
| title_fullStr | Enhanced antibody responses in CD19-Cre mice |
| title_full_unstemmed | Enhanced antibody responses in CD19-Cre mice |
| title_short | Enhanced antibody responses in CD19-Cre mice |
| title_sort | enhanced antibody responses in cd19 cre mice |
| topic | B cells BCR CD19 Antibody response Class-switch recombination |
| url | https://doi.org/10.1038/s41598-024-83954-8 |
| work_keys_str_mv | AT diogomcunha enhancedantibodyresponsesincd19cremice AT sarahernandezperez enhancedantibodyresponsesincd19cremice AT pietakmattila enhancedantibodyresponsesincd19cremice |