Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors
Collagen-derived peptides have been gaining interest due to their potential to act as DPP-IV inhibitors, which are considered to have antidiabetic effects. This study focused on extracting type I collagen from discarded southern bluefin tuna skin, confirming its structure and properties, and then me...
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Elsevier
2024-12-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X24001709 |
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| author | Erwina Safitri Olivia H. Kuziel Takeshi Nagai Masataka Saito |
| author_facet | Erwina Safitri Olivia H. Kuziel Takeshi Nagai Masataka Saito |
| author_sort | Erwina Safitri |
| collection | DOAJ |
| description | Collagen-derived peptides have been gaining interest due to their potential to act as DPP-IV inhibitors, which are considered to have antidiabetic effects. This study focused on extracting type I collagen from discarded southern bluefin tuna skin, confirming its structure and properties, and then measuring the ability of its hydrolysates to act as DPP-IV inhibitors. First, type I collagen was purified from southern bluefin tuna skin, and the constituent subunit was found to contain the α3(I) chain. Next, enzymatic hydrolysates were prepared, and their DPP-IV inhibitory activity measurements were examined and compared. Collagenase hydrolysate showed the highest DPP-IV inhibitory activity (71.8 ± 0.3 %), and considering digestive degradation, collagenase-pepsin-trypsin hydrolysate was prepared and separated into fractions via anion exchange chromatography, cartridge column, and HPLC. Notably, the 70 % methanol eluted fraction separated via cartridge column was found to have an IC50 of 0.26 ± 0.01 mg/ml. Subsequently, a novel peptide GPSGGGYDV with the highest DPP-IV inhibitory activity (IC50 value 82±0.0 µM) was purified from this fraction and found to be a competitive inhibitor of DPP-IV. This is the first study to investigate the DPP-IV inhibitory potential of type I collagen hydrolysate from southern bluefin tuna. |
| format | Article |
| id | doaj-art-acdeb47b00334fb8ab3ad3bdbed6fce4 |
| institution | Kabale University |
| issn | 2772-753X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj-art-acdeb47b00334fb8ab3ad3bdbed6fce42024-12-13T11:08:51ZengElsevierFood Chemistry Advances2772-753X2024-12-015100774Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitorsErwina Safitri0Olivia H. Kuziel1Takeshi Nagai2Masataka Saito3Laboratory of Food Science and Technology, Graduate School of Kagawa Nutrition University, Kagawa Nutrition University, Saitama, JapanLaboratory of Food Science and Technology, Graduate School of Kagawa Nutrition University, Kagawa Nutrition University, Saitama, JapanGraduate School of Agricultural Sciences, Yamagata University, Yamagata, JapanLaboratory of Food Science and Technology, Graduate School of Kagawa Nutrition University, Kagawa Nutrition University, Saitama, Japan; Corresponding author.Collagen-derived peptides have been gaining interest due to their potential to act as DPP-IV inhibitors, which are considered to have antidiabetic effects. This study focused on extracting type I collagen from discarded southern bluefin tuna skin, confirming its structure and properties, and then measuring the ability of its hydrolysates to act as DPP-IV inhibitors. First, type I collagen was purified from southern bluefin tuna skin, and the constituent subunit was found to contain the α3(I) chain. Next, enzymatic hydrolysates were prepared, and their DPP-IV inhibitory activity measurements were examined and compared. Collagenase hydrolysate showed the highest DPP-IV inhibitory activity (71.8 ± 0.3 %), and considering digestive degradation, collagenase-pepsin-trypsin hydrolysate was prepared and separated into fractions via anion exchange chromatography, cartridge column, and HPLC. Notably, the 70 % methanol eluted fraction separated via cartridge column was found to have an IC50 of 0.26 ± 0.01 mg/ml. Subsequently, a novel peptide GPSGGGYDV with the highest DPP-IV inhibitory activity (IC50 value 82±0.0 µM) was purified from this fraction and found to be a competitive inhibitor of DPP-IV. This is the first study to investigate the DPP-IV inhibitory potential of type I collagen hydrolysate from southern bluefin tuna.http://www.sciencedirect.com/science/article/pii/S2772753X24001709Southern bluefin tunaType I collagenDipeptidyl peptidase IV (DPP-IV) inhibitorAntidiabetic type 2Bioactive peptide |
| spellingShingle | Erwina Safitri Olivia H. Kuziel Takeshi Nagai Masataka Saito Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors Food Chemistry Advances Southern bluefin tuna Type I collagen Dipeptidyl peptidase IV (DPP-IV) inhibitor Antidiabetic type 2 Bioactive peptide |
| title | Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors |
| title_full | Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors |
| title_fullStr | Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors |
| title_full_unstemmed | Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors |
| title_short | Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors |
| title_sort | characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as dpp iv inhibitors |
| topic | Southern bluefin tuna Type I collagen Dipeptidyl peptidase IV (DPP-IV) inhibitor Antidiabetic type 2 Bioactive peptide |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X24001709 |
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