A single-cell 3D dynamic volume control system for chondrocytes
In articular cartilage, zone-specific cellular morphology is a typical characteristic of cartilage tissue, which is related with chondrocyte function, inflammation and osteoarthritis (OA). Chondrocyte hypertrophic phenotype is a criticle physiological process which indicates a hallmark of chondrocyt...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-10-01
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| Series: | BioTechniques |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07366205.2024.2412414 |
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| _version_ | 1841526672185098240 |
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| author | Qiang Zhang Yiyao Wang Yanjun Zhang Xiaochun Wei Weiyi Chen Quanyou Zhang |
| author_facet | Qiang Zhang Yiyao Wang Yanjun Zhang Xiaochun Wei Weiyi Chen Quanyou Zhang |
| author_sort | Qiang Zhang |
| collection | DOAJ |
| description | In articular cartilage, zone-specific cellular morphology is a typical characteristic of cartilage tissue, which is related with chondrocyte function, inflammation and osteoarthritis (OA). Chondrocyte hypertrophic phenotype is a criticle physiological process which indicates a hallmark of chondrocyte terminal differentiation and bone formation. Thus, developing a in vitro cell culture system for dynamic regulation of single chondrocyte volume at a three-dimensional (3D) level is particularly necessary for understanding how physical cues of matrix microenvironment regulate chondrocyte fate and the degeneration of articular cartilage. Here, based on the soft lithography techniques, we have constructed well-defined single-cell 3D dynamic volume control system to recapitulate the physiological matrix microenvironment of single chondrocyte niche. The results of finite element analysis indicated that the stress and strain distribution in the cell culture region is homogeneous during the stretching process. Additionally, 3D dynamic volume expansion and compression of single cells in physiological or hyperphysiological can be realized in this cell culture system. Our device for single-cell 3D dynamic culture provides a microphysiological culture system for chondrocytes to explore the mechanisms of cartilage hypertrophy, as well as develops a new paradigm for functional cartilage tissue engineering and regenerative medicine. |
| format | Article |
| id | doaj-art-ac83b5d36ef6461c9abd960434a09773 |
| institution | Kabale University |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-ac83b5d36ef6461c9abd960434a097732025-01-16T15:42:48ZengTaylor & Francis GroupBioTechniques0736-62051940-98182024-10-01761049550410.1080/07366205.2024.2412414A single-cell 3D dynamic volume control system for chondrocytesQiang Zhang0Yiyao Wang1Yanjun Zhang2Xiaochun Wei3Weiyi Chen4Quanyou Zhang5College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, ChinaCollege of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, ChinaCollege of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, ChinaDepartment of Orthopaedics, The Second Hospital of Shanxi Medical University, Shanxi Key Laboratory of Bone & Soft Tissue Injury Repair, Shanxi Medical University, Taiyuan, 030001, ChinaCollege of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, ChinaCollege of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, ChinaIn articular cartilage, zone-specific cellular morphology is a typical characteristic of cartilage tissue, which is related with chondrocyte function, inflammation and osteoarthritis (OA). Chondrocyte hypertrophic phenotype is a criticle physiological process which indicates a hallmark of chondrocyte terminal differentiation and bone formation. Thus, developing a in vitro cell culture system for dynamic regulation of single chondrocyte volume at a three-dimensional (3D) level is particularly necessary for understanding how physical cues of matrix microenvironment regulate chondrocyte fate and the degeneration of articular cartilage. Here, based on the soft lithography techniques, we have constructed well-defined single-cell 3D dynamic volume control system to recapitulate the physiological matrix microenvironment of single chondrocyte niche. The results of finite element analysis indicated that the stress and strain distribution in the cell culture region is homogeneous during the stretching process. Additionally, 3D dynamic volume expansion and compression of single cells in physiological or hyperphysiological can be realized in this cell culture system. Our device for single-cell 3D dynamic culture provides a microphysiological culture system for chondrocytes to explore the mechanisms of cartilage hypertrophy, as well as develops a new paradigm for functional cartilage tissue engineering and regenerative medicine.https://www.tandfonline.com/doi/10.1080/07366205.2024.2412414cell volumechondrocytemechanical stimulusmorphologysingle-cell niche |
| spellingShingle | Qiang Zhang Yiyao Wang Yanjun Zhang Xiaochun Wei Weiyi Chen Quanyou Zhang A single-cell 3D dynamic volume control system for chondrocytes BioTechniques cell volume chondrocyte mechanical stimulus morphology single-cell niche |
| title | A single-cell 3D dynamic volume control system for chondrocytes |
| title_full | A single-cell 3D dynamic volume control system for chondrocytes |
| title_fullStr | A single-cell 3D dynamic volume control system for chondrocytes |
| title_full_unstemmed | A single-cell 3D dynamic volume control system for chondrocytes |
| title_short | A single-cell 3D dynamic volume control system for chondrocytes |
| title_sort | single cell 3d dynamic volume control system for chondrocytes |
| topic | cell volume chondrocyte mechanical stimulus morphology single-cell niche |
| url | https://www.tandfonline.com/doi/10.1080/07366205.2024.2412414 |
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