Investigation of in vitro and in vivo therapeutic activity of Tarantula cubensis extract (Theranekron®) on Leishmania major
Background & objectives: Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease caused by several species of the protozoan parasite Leishmania. The need for new anti-leishmanial drugs for the treatment of CL is complicated by factors such as high cost, toxicity, potential for resistanc...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-11-01
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| Series: | Journal of Vector Borne Diseases |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/JVBD.jvbd_55_24 |
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| Summary: | Background & objectives:
Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease caused by several species of the protozoan parasite Leishmania. The need for new anti-leishmanial drugs for the treatment of CL is complicated by factors such as high cost, toxicity, potential for resistance and limited long-term use of existing anti-leishmanial drugs. The aim of this study was to investigate the therapeutic effect of Tarantula cubensis alcoholic extract (TCE), which has been shown to have wound-healing, anti-inflammatory, regenerative, resolving and epithelialising effects, on Leishmania major promastigotes in vitro and in vivo in an experimental mouse model of CL.
Methods:
The effect of TCE on L. major promastigotes in vitro was investigated after determining non-cytotoxic concentrations of TCE using the XTT method. To establish a CL model, L. major amastigotes were injected into the paws of BALB/c mice. Lesion size and histopathological evaluation were used to assess the effect of treatment.
Results:
TCE was found to be effective against L. major promastigotes at 24 h and 48 h at concentrations 250 μg/ mL, 125 μg/mL and 62.5 μg/mL (P<0.05). TCE was found to be more effective than meglumine antimonate in treating CL in the experimentally induced CL model in BALB/c mice.
Interpretation & conclusion:
The results suggest that TCE holds promising potential as a therapeutic agent for the treatment of CL. However, further extensive investigations are required to substantiate and expand our understanding in this area. |
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| ISSN: | 0972-9062 |