Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkey
<b>Aim:</b> The present study was on the detection of Salmonella spp. and two important Salmonella serotypes (S. Thyphimurium and S. Enteritidis) in chicken meat and giblets and also determination of antimicrobial resistance of the isolates.<p> <b>Materials and Methods:</b...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1215 |
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| Summary: | <b>Aim:</b> The present study was on the detection of Salmonella spp. and two
important Salmonella serotypes (S. Thyphimurium and S. Enteritidis) in
chicken meat and giblets and also determination of antimicrobial resistance
of the isolates.<p>
<b>Materials and Methods:</b> In this study, livers (n=40), gizzards (n=40),
hearts (n=30), skins (n=30), drumsticks (n=10) and wings (n=20) were
collected from supermarkets and butcher shops in Konya, Turkey. The
samples were analyzed by Classical Cultural Technique. Molecular
confirmation of the suspicious colonies was carried out using Inv-A gene-
based PCR. Flic-C and IE-1 primers were used by duplex PCR for S.
Thyphimurium and S. Enteritidis respectively. Antibiotic resistance of
the isolates was determined by the disk diffusion method.<p>
<b>Results:</b> Forty-three (25.29 %) of 170 samples were positive for Salmonella
spp. According to the d-PCR assay, neither S. Thyphimurium nor
S. Enteritidis was not detected. The resistance to clindamycin, oxacillin,
teicoplanin were evident 100 % and resistance to vancomycin (79.1
%), erythromycin (79.1 %), nalidixic acid (65.1 %), penicillin G (60.5
%) cephalothin (48.8 %), sulfamethoxazole-trimethoprim (37.2 %),
tetracycline (37.2 %), ampicillin (23.3 %), kanamycin (18.6 %), chloramphenicol
(11.6 %) amikacin, cephazoline, ciprofloxacin, gentamycin
(4.7 %) was also detected. All isolates were susceptible to amoxicillin/
clavulanic acid and cefixime.<p>
<b>Conclusion:</b> The results indicated that S. Enteritidis and S. Typhimurium
were not identified and it was considered satisfactory in terms of
public health. It should be still important to note the studies to identify
species with lower pathogenic incidences for legal legislation. Furthermore,
even the most common pathogenic species cannot be detected,
the results of antibiotic resistance in isolates were noteworthy for antibiotic
surveillance database. |
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| ISSN: | 1309-6958 2146-1953 |