Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad City
L-methioninase, a pyridoxal 5′-phosphate dependent enzyme that catalyze degradation of L-methionine to ammonia, methanethiol and α -ketobutyrate. L-methioninase had received a lot of interest for its importance, so to improve the production of L-methioninase, media optimization was done. In this st...
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Corporation of Research and Industrial Development
2024-12-01
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| Series: | Iraqi Journal of Industrial Research |
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| Online Access: | http://ijoir.gov.iq/ijoir/index.php/jou/article/view/494 |
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| author | Zahraa Mohammed Wahib Sahira Nsayef Muslim Farha A. Ali Shafi |
| author_facet | Zahraa Mohammed Wahib Sahira Nsayef Muslim Farha A. Ali Shafi |
| author_sort | Zahraa Mohammed Wahib |
| collection | DOAJ |
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L-methioninase, a pyridoxal 5′-phosphate dependent enzyme that catalyze degradation of L-methionine to ammonia, methanethiol and α -ketobutyrate. L-methioninase had received a lot of interest for its importance, so to improve the production of L-methioninase, media optimization was done. In this study, clinical isolates of gram-negative bacteria have been collected from several hospitals located in Baghdad city, these isolates were Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii and Serratia marcescens. The screening for detecting L-methioninase production was performed by semi-quantitative and quantitative analysis, the results revealed that out of all obtained isolates, 31(41.33%) were L-methioninase producers from semi-quantitative screening while by quantitative method only 16 isolates out of these 31 isolates revealed specific activity ranged from 0.19 to 1.15 U/mg and the maximum specific activity was for E. coli U8, which was chosen as best producer isolates. The L-methioninase activity reached its maximum level when E. coli U8 was cultivated with the best conditions, which is consisted of using modified mineral salt M9 broth medium supplemented with L-methionine mixed with galactose (2 g/L) as carbon source and L-glutamine (1.5 g/L) and incubated at 37°C for 48 hours at pH 7.
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| format | Article |
| id | doaj-art-ab782d8e711f482b81eb270ca70d76c6 |
| institution | Kabale University |
| issn | 2788-712X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Corporation of Research and Industrial Development |
| record_format | Article |
| series | Iraqi Journal of Industrial Research |
| spelling | doaj-art-ab782d8e711f482b81eb270ca70d76c62024-12-15T05:02:22ZengCorporation of Research and Industrial DevelopmentIraqi Journal of Industrial Research2788-712X2024-12-0111310.53523/ijoirVol11I3ID494494Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad CityZahraa Mohammed Wahib0Sahira Nsayef Muslim1Farha A. Ali Shafi2Department of Biology, College of Science, Mustansiriyah University, IraqDepartment of Biology, College of Science, Mustansiriyah University, IraqDepartment of Biology, College of Science, Mustansiriyah University, Iraq L-methioninase, a pyridoxal 5′-phosphate dependent enzyme that catalyze degradation of L-methionine to ammonia, methanethiol and α -ketobutyrate. L-methioninase had received a lot of interest for its importance, so to improve the production of L-methioninase, media optimization was done. In this study, clinical isolates of gram-negative bacteria have been collected from several hospitals located in Baghdad city, these isolates were Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii and Serratia marcescens. The screening for detecting L-methioninase production was performed by semi-quantitative and quantitative analysis, the results revealed that out of all obtained isolates, 31(41.33%) were L-methioninase producers from semi-quantitative screening while by quantitative method only 16 isolates out of these 31 isolates revealed specific activity ranged from 0.19 to 1.15 U/mg and the maximum specific activity was for E. coli U8, which was chosen as best producer isolates. The L-methioninase activity reached its maximum level when E. coli U8 was cultivated with the best conditions, which is consisted of using modified mineral salt M9 broth medium supplemented with L-methionine mixed with galactose (2 g/L) as carbon source and L-glutamine (1.5 g/L) and incubated at 37°C for 48 hours at pH 7. http://ijoir.gov.iq/ijoir/index.php/jou/article/view/494Gram-negative bacteriaL-methioninaseOptimization conditions |
| spellingShingle | Zahraa Mohammed Wahib Sahira Nsayef Muslim Farha A. Ali Shafi Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad City Iraqi Journal of Industrial Research Gram-negative bacteria L-methioninase Optimization conditions |
| title | Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad City |
| title_full | Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad City |
| title_fullStr | Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad City |
| title_full_unstemmed | Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad City |
| title_short | Screening and Optimization of L-Methioninase Production by Gram-Negative Bacteria from Various Hospitals in Baghdad City |
| title_sort | screening and optimization of l methioninase production by gram negative bacteria from various hospitals in baghdad city |
| topic | Gram-negative bacteria L-methioninase Optimization conditions |
| url | http://ijoir.gov.iq/ijoir/index.php/jou/article/view/494 |
| work_keys_str_mv | AT zahraamohammedwahib screeningandoptimizationoflmethioninaseproductionbygramnegativebacteriafromvarioushospitalsinbaghdadcity AT sahiransayefmuslim screeningandoptimizationoflmethioninaseproductionbygramnegativebacteriafromvarioushospitalsinbaghdadcity AT farhaaalishafi screeningandoptimizationoflmethioninaseproductionbygramnegativebacteriafromvarioushospitalsinbaghdadcity |