MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensis
Peptides play regulatory roles in various plant development and defence processes. They function as molecular messengers that detect threats and trigger defence responses. This study aimed to identify the genes encoding endogenous plant elicitor peptide precursors (PROPEPs) in bananas and their role...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2025-07-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-202503-0005_mapep1-and-mbpep1-as-plant-elicitor-peptides-in-banana-confer-resistance-to-banana-blood-disease-caused-by-ra.php |
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| author | Praphat Kawicha Ladawan Rattanapolsan Ratri Boonruangrod Yube Yamaguchi Kusavadee Sangdee Aphidech Sangdee Thanwanit Thanyasiriwat |
| author_facet | Praphat Kawicha Ladawan Rattanapolsan Ratri Boonruangrod Yube Yamaguchi Kusavadee Sangdee Aphidech Sangdee Thanwanit Thanyasiriwat |
| author_sort | Praphat Kawicha |
| collection | DOAJ |
| description | Peptides play regulatory roles in various plant development and defence processes. They function as molecular messengers that detect threats and trigger defence responses. This study aimed to identify the genes encoding endogenous plant elicitor peptide precursors (PROPEPs) in bananas and their role in inducing resistance to Ralstonia syzygii subsp. celebesensis (Rsc). Two precursor genes, MaPROPEP1 and MbPROPEP1, were discovered and predicted to encode the precursor proteins of elicitor peptides, namely, MaPep1 and MbPep1. Both elicitor peptides contained 23 amino acids of the active elicitor peptide, which activated innate immune responses in banana resistance to Rsc. The disease assessment was conducted by inoculating banana plants with Rsc isolate MY4101 using the root-stabbing method. The results demonstrated that MaPep1 and MbPep1 pretreatment enhanced resistance to banana blood disease, as indicated by reduced disease severity and the absence of wilting for 7 days after infection. The expression of the MaPROPEP1, MbPROPEP1, MaLOX7, and Pr-10 genes was evaluated using qPCR and found to be upregulated by MaPep1 and MbPep1 injection followed by Rsc infection in aboveground banana tissues within 7 days. These findings prove that MaPep1 and MbPep1 are members of the Pep family and exhibit conserved functions across various plant species. This approach may be used to develop strategies for enhancing disease resistance in banana cultivation. |
| format | Article |
| id | doaj-art-ab28b876e73d42f0b4f20ef3c16b9c31 |
| institution | Kabale University |
| issn | 1212-2580 1805-9341 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj-art-ab28b876e73d42f0b4f20ef3c16b9c312025-08-20T03:51:44ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412025-07-0161326227710.17221/179/2024-PPSpps-202503-0005MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensisPraphat Kawicha0https://orcid.org/0000-0003-4230-2720Ladawan Rattanapolsan1Ratri Boonruangrod2https://orcid.org/0000-0001-9294-193XYube Yamaguchi3Kusavadee Sangdee4https://orcid.org/0000-0003-0481-8267Aphidech Sangdee5https://orcid.org/0000-0003-4401-8985Thanwanit Thanyasiriwat6https://orcid.org/0000-0002-0234-120XPlant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham, ThailanDepartment of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, ThailandGraduate School of Agriculture, Department of Agricultural Biology, School of Agriculture, Osaka Metropolitan University, Sakai, JapanPreclinical Group, Faculty of Medicine, Mahasarakham University, Maha Sarakham, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham, ThailanPlant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, ThailandPeptides play regulatory roles in various plant development and defence processes. They function as molecular messengers that detect threats and trigger defence responses. This study aimed to identify the genes encoding endogenous plant elicitor peptide precursors (PROPEPs) in bananas and their role in inducing resistance to Ralstonia syzygii subsp. celebesensis (Rsc). Two precursor genes, MaPROPEP1 and MbPROPEP1, were discovered and predicted to encode the precursor proteins of elicitor peptides, namely, MaPep1 and MbPep1. Both elicitor peptides contained 23 amino acids of the active elicitor peptide, which activated innate immune responses in banana resistance to Rsc. The disease assessment was conducted by inoculating banana plants with Rsc isolate MY4101 using the root-stabbing method. The results demonstrated that MaPep1 and MbPep1 pretreatment enhanced resistance to banana blood disease, as indicated by reduced disease severity and the absence of wilting for 7 days after infection. The expression of the MaPROPEP1, MbPROPEP1, MaLOX7, and Pr-10 genes was evaluated using qPCR and found to be upregulated by MaPep1 and MbPep1 injection followed by Rsc infection in aboveground banana tissues within 7 days. These findings prove that MaPep1 and MbPep1 are members of the Pep family and exhibit conserved functions across various plant species. This approach may be used to develop strategies for enhancing disease resistance in banana cultivation.https://pps.agriculturejournals.cz/artkey/pps-202503-0005_mapep1-and-mbpep1-as-plant-elicitor-peptides-in-banana-confer-resistance-to-banana-blood-disease-caused-by-ra.phpimmunitypropep genespathogenesis-related genesplant immunity |
| spellingShingle | Praphat Kawicha Ladawan Rattanapolsan Ratri Boonruangrod Yube Yamaguchi Kusavadee Sangdee Aphidech Sangdee Thanwanit Thanyasiriwat MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensis Plant Protection Science immunity propep genes pathogenesis-related genes plant immunity |
| title | MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensis |
| title_full | MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensis |
| title_fullStr | MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensis |
| title_full_unstemmed | MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensis |
| title_short | MaPep1 and MbPep1, as plant elicitor peptides in banana, confer resistance to banana blood disease caused by Ralstonia syzygii subsp. celebesensis |
| title_sort | mapep1 and mbpep1 as plant elicitor peptides in banana confer resistance to banana blood disease caused by ralstonia syzygii subsp celebesensis |
| topic | immunity propep genes pathogenesis-related genes plant immunity |
| url | https://pps.agriculturejournals.cz/artkey/pps-202503-0005_mapep1-and-mbpep1-as-plant-elicitor-peptides-in-banana-confer-resistance-to-banana-blood-disease-caused-by-ra.php |
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