Comparative Potential of Histological Effect in Antioxidants to Prevent Doxorubicin Induced Toxicity in Male Infertility in Rats

Objective: This research intended to compare histological impacts of various antioxidants in preventing doxorubicin-induced testicular toxicity and subsequent male infertility in rats. Study Design: Cross-sectional study. Place and Duration of Study: This study was conducted at the Animal House and...

Full description

Saved in:
Bibliographic Details
Main Authors: Rahmat Ullah Jan, Salman Yunas, Nighat Ara, Adnan Badar, Mohammad Amjid, Shahid Fareed, Jurate Rinkeviciute
Format: Article
Language:English
Published: National University of Medical Sciences 2024-11-01
Series:Life and Science
Subjects:
Online Access:https://ojs.lifenscience.org/index.php/life-and-science/article/view/736
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1846150844665298944
author Rahmat Ullah Jan
Salman Yunas
Nighat Ara
Adnan Badar
Mohammad Amjid
Shahid Fareed
Jurate Rinkeviciute
author_facet Rahmat Ullah Jan
Salman Yunas
Nighat Ara
Adnan Badar
Mohammad Amjid
Shahid Fareed
Jurate Rinkeviciute
author_sort Rahmat Ullah Jan
collection DOAJ
description Objective: This research intended to compare histological impacts of various antioxidants in preventing doxorubicin-induced testicular toxicity and subsequent male infertility in rats. Study Design: Cross-sectional study. Place and Duration of Study: This study was conducted at the Animal House and Histopathology Laboratory of Peshawar Medical College, Peshawar, Pakistan from May 2023 to April 2024. Methods: An overall of 120 male rats were included in the research. The rats were divided into six groups: the control group, the doxorubicin only group, and four treatment groups receiving doxorubicin along with different antioxidants. The antioxidants administered were Vitamin C, Vitamin E, Coenzyme Q10, and Selenium. Histological analyses were performed on testicular tissues post-treatment to assess the extent of damage and protective effects of the antioxidants. Results: The doxorubicin only group showed statistically significant histological damage, including marked reductions in spermatogenesis and degeneration of seminiferous tubules. Antioxidant treated groups demonstrated significant protective effects, with the Selenium group exhibiting the highest level of protection, closely resembling the control group, followed by Vitamin E and Coenzyme Q10, which also provided substantial preservation of testicular structure. Conclusion: The study concluded that antioxidants, particularly Selenium, Vitamin E, and Coenzyme Q10, provided significant protection against doxorubicin induced testicular toxicity in rats. These findings suggested possible use of these antioxidants in mitigating male infertility associated with doxorubicin therapy. How to cite this: Jan RU, Yunas S, Ara N, Amjid M, Fareed S, Rinkeviciute J, Badar A. Comparative Potential Of Histological Effect In Antioxidants To Prevent Doxorubicin Induced Toxicity In Male Infertility In Rats. Life and Science. 2024; 5(4): 544-551. doi: http://doi.org/10.37185/LnS.1.1.736
format Article
id doaj-art-ab142984c7cc4d30a160ff7d94197d49
institution Kabale University
issn 2521-0475
2708-2970
language English
publishDate 2024-11-01
publisher National University of Medical Sciences
record_format Article
series Life and Science
spelling doaj-art-ab142984c7cc4d30a160ff7d94197d492024-11-28T07:37:58ZengNational University of Medical SciencesLife and Science2521-04752708-29702024-11-0154080810.37185/LnS.1.1.736736Comparative Potential of Histological Effect in Antioxidants to Prevent Doxorubicin Induced Toxicity in Male Infertility in RatsRahmat Ullah Jan0Salman Yunas1Nighat Ara2Adnan Badar3Mohammad Amjid4Shahid Fareed5Jurate Rinkeviciute6Muhammad College of Medicine Peshawar, PakistanSwat Medical College Swat, PakistanNowshera Medical College Nowshera, PakistanSwat Medical College Swat, PakistanTHQ Hospital Shabqadar, PakistanMuhammad College of Medicine Peshawar, PakistanNational Cancer Institute, Vilnius, LithuaniaObjective: This research intended to compare histological impacts of various antioxidants in preventing doxorubicin-induced testicular toxicity and subsequent male infertility in rats. Study Design: Cross-sectional study. Place and Duration of Study: This study was conducted at the Animal House and Histopathology Laboratory of Peshawar Medical College, Peshawar, Pakistan from May 2023 to April 2024. Methods: An overall of 120 male rats were included in the research. The rats were divided into six groups: the control group, the doxorubicin only group, and four treatment groups receiving doxorubicin along with different antioxidants. The antioxidants administered were Vitamin C, Vitamin E, Coenzyme Q10, and Selenium. Histological analyses were performed on testicular tissues post-treatment to assess the extent of damage and protective effects of the antioxidants. Results: The doxorubicin only group showed statistically significant histological damage, including marked reductions in spermatogenesis and degeneration of seminiferous tubules. Antioxidant treated groups demonstrated significant protective effects, with the Selenium group exhibiting the highest level of protection, closely resembling the control group, followed by Vitamin E and Coenzyme Q10, which also provided substantial preservation of testicular structure. Conclusion: The study concluded that antioxidants, particularly Selenium, Vitamin E, and Coenzyme Q10, provided significant protection against doxorubicin induced testicular toxicity in rats. These findings suggested possible use of these antioxidants in mitigating male infertility associated with doxorubicin therapy. How to cite this: Jan RU, Yunas S, Ara N, Amjid M, Fareed S, Rinkeviciute J, Badar A. Comparative Potential Of Histological Effect In Antioxidants To Prevent Doxorubicin Induced Toxicity In Male Infertility In Rats. Life and Science. 2024; 5(4): 544-551. doi: http://doi.org/10.37185/LnS.1.1.736https://ojs.lifenscience.org/index.php/life-and-science/article/view/736histology, selenium, spermatogenesis, vitamin c, vitamin e.
spellingShingle Rahmat Ullah Jan
Salman Yunas
Nighat Ara
Adnan Badar
Mohammad Amjid
Shahid Fareed
Jurate Rinkeviciute
Comparative Potential of Histological Effect in Antioxidants to Prevent Doxorubicin Induced Toxicity in Male Infertility in Rats
Life and Science
histology, selenium, spermatogenesis, vitamin c, vitamin e.
title Comparative Potential of Histological Effect in Antioxidants to Prevent Doxorubicin Induced Toxicity in Male Infertility in Rats
title_full Comparative Potential of Histological Effect in Antioxidants to Prevent Doxorubicin Induced Toxicity in Male Infertility in Rats
title_fullStr Comparative Potential of Histological Effect in Antioxidants to Prevent Doxorubicin Induced Toxicity in Male Infertility in Rats
title_full_unstemmed Comparative Potential of Histological Effect in Antioxidants to Prevent Doxorubicin Induced Toxicity in Male Infertility in Rats
title_short Comparative Potential of Histological Effect in Antioxidants to Prevent Doxorubicin Induced Toxicity in Male Infertility in Rats
title_sort comparative potential of histological effect in antioxidants to prevent doxorubicin induced toxicity in male infertility in rats
topic histology, selenium, spermatogenesis, vitamin c, vitamin e.
url https://ojs.lifenscience.org/index.php/life-and-science/article/view/736
work_keys_str_mv AT rahmatullahjan comparativepotentialofhistologicaleffectinantioxidantstopreventdoxorubicininducedtoxicityinmaleinfertilityinrats
AT salmanyunas comparativepotentialofhistologicaleffectinantioxidantstopreventdoxorubicininducedtoxicityinmaleinfertilityinrats
AT nighatara comparativepotentialofhistologicaleffectinantioxidantstopreventdoxorubicininducedtoxicityinmaleinfertilityinrats
AT adnanbadar comparativepotentialofhistologicaleffectinantioxidantstopreventdoxorubicininducedtoxicityinmaleinfertilityinrats
AT mohammadamjid comparativepotentialofhistologicaleffectinantioxidantstopreventdoxorubicininducedtoxicityinmaleinfertilityinrats
AT shahidfareed comparativepotentialofhistologicaleffectinantioxidantstopreventdoxorubicininducedtoxicityinmaleinfertilityinrats
AT juraterinkeviciute comparativepotentialofhistologicaleffectinantioxidantstopreventdoxorubicininducedtoxicityinmaleinfertilityinrats