Natural Bactericidal Effects of <i>Psoralea glandulosa</i> Essential Oil for the Control of Bacterial Canker and Speck in Tomato

Bacterial canker and bacterial speck are diseases affecting tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> and <i>Pseudomonas syringae</i> pv. <i>tomato</i>, respectively. These diseases are considered a serious threat wi...

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Main Authors: Iván Montenegro, Miryam Valenzuela Ormeño, Michael Seeger, Ximena Besoain, Patricio Godoy, Enrique Werner, Nelson Caro, Yusser Olguín, Manuel Valenzuela, Valentina Silva, Alejandro Madrid
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Language:English
Published: MDPI AG 2024-11-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/11/2615
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author Iván Montenegro
Miryam Valenzuela Ormeño
Michael Seeger
Ximena Besoain
Patricio Godoy
Enrique Werner
Nelson Caro
Yusser Olguín
Manuel Valenzuela
Valentina Silva
Alejandro Madrid
author_facet Iván Montenegro
Miryam Valenzuela Ormeño
Michael Seeger
Ximena Besoain
Patricio Godoy
Enrique Werner
Nelson Caro
Yusser Olguín
Manuel Valenzuela
Valentina Silva
Alejandro Madrid
author_sort Iván Montenegro
collection DOAJ
description Bacterial canker and bacterial speck are diseases affecting tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> and <i>Pseudomonas syringae</i> pv. <i>tomato</i>, respectively. These diseases are considered a serious threat with a strong impact on tomato production and marketing worldwide, especially because of their reduced sensitivity to traditional controls. This work reports the chemical composition of the essential oil (EO) of <i>Psoralea glandulosa</i> and investigates its in vitro antimicrobial activity, along with its main compound, against three strains of <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> (<i>CmVC533</i>, <i>CmVLC78</i>, and <i>CmVQ59</i>) and one strain of <i>Pseudomonas syringae</i> pv. <i>tomato</i> (<i>Pst</i>). The results indicate that both the EO and bakuchiol have significant antibacterial capacity, especially the EO, which reaches a minimum inhibitory concentration (MIC) between 4–16 µg/mL and 128 µg/mL, and a minimum bactericidal concentration (MBC) between 8–16 µg/mL and 128 µg/mL for the strains of <i>C. michiganensis</i> subsp. <i>michiganensis</i> and <i>P. syringae</i> pv. <i>tomato</i>, respectively. The EO and bakuchiol also had an inhibitory effect when applied directly onto plates seeded with <i>C. michiganensis</i> subsp. <i>michiganensis</i>. Overall, the results from this study should be verified in the near future by <i>in vivo</i> studies.
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spelling doaj-art-aa7c59c1058240c1b3b38eee6bffb35f2024-11-26T17:44:41ZengMDPI AGAgronomy2073-43952024-11-011411261510.3390/agronomy14112615Natural Bactericidal Effects of <i>Psoralea glandulosa</i> Essential Oil for the Control of Bacterial Canker and Speck in TomatoIván Montenegro0Miryam Valenzuela Ormeño1Michael Seeger2Ximena Besoain3Patricio Godoy4Enrique Werner5Nelson Caro6Yusser Olguín7Manuel Valenzuela8Valentina Silva9Alejandro Madrid10Center of Interdisciplinary Biomedical and Engineering Research for Health (MEDING), Escuela de Obstetricia y Puericultura, Facultad de Medicina, Universidad de Valparaíso, Angamos 655, Reñaca, Viña del Mar 2520000, ChileMillennium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, Valparaíso 2390123, ChileMillennium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, Valparaíso 2390123, ChileMillennium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, Valparaíso 2390123, ChileInstituto de Microbiología Clínica, Facultad de Medicina, Universidad Austral de Chile, Los Laureles s/n, Isla Teja, Valdivia 5090000, ChileDepartamento de Ciencias Básicas, Campus Fernando May, Universidad del Bío-Bío, Avda. Andrés Bello 720, Casilla 447, Chillán 3780000, ChileCentro de Investigación Austral Biotech, Facultad de Ciencias, Universidad Santo Tomás, Avda. Ejército 146, Santiago 8320000, ChileDepartamento de Química y Medio Ambiente, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, ChileLaboratorio de Microbiología Celular, Facultad de Medicina y Ciencias de la Salud, Universidad Central de Chile, Santiago 8370292, ChileLaboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Departamento de Ciencias y Geografía, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2360004, ChileLaboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Departamento de Ciencias y Geografía, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2360004, ChileBacterial canker and bacterial speck are diseases affecting tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> and <i>Pseudomonas syringae</i> pv. <i>tomato</i>, respectively. These diseases are considered a serious threat with a strong impact on tomato production and marketing worldwide, especially because of their reduced sensitivity to traditional controls. This work reports the chemical composition of the essential oil (EO) of <i>Psoralea glandulosa</i> and investigates its in vitro antimicrobial activity, along with its main compound, against three strains of <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> (<i>CmVC533</i>, <i>CmVLC78</i>, and <i>CmVQ59</i>) and one strain of <i>Pseudomonas syringae</i> pv. <i>tomato</i> (<i>Pst</i>). The results indicate that both the EO and bakuchiol have significant antibacterial capacity, especially the EO, which reaches a minimum inhibitory concentration (MIC) between 4–16 µg/mL and 128 µg/mL, and a minimum bactericidal concentration (MBC) between 8–16 µg/mL and 128 µg/mL for the strains of <i>C. michiganensis</i> subsp. <i>michiganensis</i> and <i>P. syringae</i> pv. <i>tomato</i>, respectively. The EO and bakuchiol also had an inhibitory effect when applied directly onto plates seeded with <i>C. michiganensis</i> subsp. <i>michiganensis</i>. Overall, the results from this study should be verified in the near future by <i>in vivo</i> studies.https://www.mdpi.com/2073-4395/14/11/2615essential oil<i>Psoralea glandulosa</i><i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i>bakuchiol<i>Pseudomonas syringae</i> pv. <i>tomato</i>
spellingShingle Iván Montenegro
Miryam Valenzuela Ormeño
Michael Seeger
Ximena Besoain
Patricio Godoy
Enrique Werner
Nelson Caro
Yusser Olguín
Manuel Valenzuela
Valentina Silva
Alejandro Madrid
Natural Bactericidal Effects of <i>Psoralea glandulosa</i> Essential Oil for the Control of Bacterial Canker and Speck in Tomato
Agronomy
essential oil
<i>Psoralea glandulosa</i>
<i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i>
bakuchiol
<i>Pseudomonas syringae</i> pv. <i>tomato</i>
title Natural Bactericidal Effects of <i>Psoralea glandulosa</i> Essential Oil for the Control of Bacterial Canker and Speck in Tomato
title_full Natural Bactericidal Effects of <i>Psoralea glandulosa</i> Essential Oil for the Control of Bacterial Canker and Speck in Tomato
title_fullStr Natural Bactericidal Effects of <i>Psoralea glandulosa</i> Essential Oil for the Control of Bacterial Canker and Speck in Tomato
title_full_unstemmed Natural Bactericidal Effects of <i>Psoralea glandulosa</i> Essential Oil for the Control of Bacterial Canker and Speck in Tomato
title_short Natural Bactericidal Effects of <i>Psoralea glandulosa</i> Essential Oil for the Control of Bacterial Canker and Speck in Tomato
title_sort natural bactericidal effects of i psoralea glandulosa i essential oil for the control of bacterial canker and speck in tomato
topic essential oil
<i>Psoralea glandulosa</i>
<i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i>
bakuchiol
<i>Pseudomonas syringae</i> pv. <i>tomato</i>
url https://www.mdpi.com/2073-4395/14/11/2615
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