Morphological and molecular changes in renal tissue following experimental unilateral ureteral obstruction in rat kidneys
This study was designed to investigate the pathological, molecular, and biochemical changes subjected to experimental unilateral ureteral obstruction (UUO) during the acute phase, specifically within a seven-day period. The kidney"s reaction to obstructions, such as unilateral ureteral obstruct...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2024-11-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3150 |
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| Summary: | This study was designed to investigate the pathological, molecular, and biochemical
changes subjected to experimental unilateral ureteral obstruction (UUO) during the
acute phase, specifically within a seven-day period. The kidney"s reaction to obstructions,
such as unilateral ureteral obstruction (UUO), is highly complex and involves a dynamic
interplay of various molecular processes. A total of 72 Wistar Albino rats were divided
into nine groups, each consisting of eight rats. The left kidneys of the first seven groups
underwent UUO surgery, with one group being necropsied daily. All left kidneys were
examined using histopathological, immunohistochemical, biochemical, and molecular
methods. On the first day of unilateral ureteral obstruction (UUO), significant
pathological and biochemical changes were observed in the kidneys. These included
the formation of cystic dilated tubules, a decrease in cyclooxygenase-2 (COX-2) gene
expression, and a reduction in glutathione peroxidase (GSH-Px) levels. By the second
day, hyperemia, increased tumor necrosis factor-alpha (TNFα) gene expression, and
elevated malondialdehyde (MDA) levels were evident. In the third day; there was mild
interstitial mononuclear cell infiltration roteinaceous filtrate in tubules, heightened
transforming growth factor beta 1 (TGF-β1) gene and protein expression, and increased
angiotensin II (ANG-II) and interleukin-10 (IL-10) protein expression. By day five,
kidneys subjected to UUO exhibited hydropic degeneration, TNF-α protein expression,
and anti-RELA antibody expression. On the sixth day, significant increase in IL-10 gene
expression was noted. In conclusion; these results provide valuable insights for future
studies on UUO pathogenesis and research into potential treatment modalities. |
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| ISSN: | 1309-2251 |