Detection of soybean by real-time PCR in the samples subjected to deep technological processing
During deep technological processing, DNA of food product components (specifically, in canned foods) is subjected to strong degradation, which makes the PCR-based food components identification more difficult. In this work, a primer-probe system is proposed, which was selected for the multi-copy reg...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The V.M. Gorbatov All-Russian Meat Research Institute
2019-12-01
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| Series: | Теория и практика переработки мяса |
| Subjects: | |
| Online Access: | https://www.meatjournal.ru/jour/article/view/126 |
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| author | K. A. Kurbakov E. A. Konorov V. N. Zhulinkova M. Yu. Minaev |
| author_facet | K. A. Kurbakov E. A. Konorov V. N. Zhulinkova M. Yu. Minaev |
| author_sort | K. A. Kurbakov |
| collection | DOAJ |
| description | During deep technological processing, DNA of food product components (specifically, in canned foods) is subjected to strong degradation, which makes the PCR-based food components identification more difficult. In this work, a primer-probe system is proposed, which was selected for the multi-copy region of long terminal repeat (LTR) of soybean (Glycine max). We confirmed its high sensitivity and specificity for soybean detection by real-time PCR. Using the selected system, we successfully analyzed the samples of meat-and-plant canned foods and other food products subjected to deep technological processing — tofu, preserved tofu, soy sauces, confectionary products containing soy lecithin. To compare with these samples, real-time PCR was carried out using the primer-probe system selected for the single-copy le1 gene. In terms of sensitivity, the use of the primer-probe system specific to the single-copy region was significantly inferior to the primer-probe system specific to the LTR region. The difference in the rate of degradation of these genomic DNA regions of Glycine max was found. |
| format | Article |
| id | doaj-art-aa6be250c7fc4b9bb7af707af0319845 |
| institution | Kabale University |
| issn | 2414-438X 2414-441X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | The V.M. Gorbatov All-Russian Meat Research Institute |
| record_format | Article |
| series | Теория и практика переработки мяса |
| spelling | doaj-art-aa6be250c7fc4b9bb7af707af03198452025-08-20T03:59:57ZengThe V.M. Gorbatov All-Russian Meat Research InstituteТеория и практика переработки мяса2414-438X2414-441X2019-12-0144232710.21323/2414-438X-2019-4-4-23-27105Detection of soybean by real-time PCR in the samples subjected to deep technological processingK. A. Kurbakov0E. A. Konorov1V. N. Zhulinkova2M. Yu. Minaev3V. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of SciencesV. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of SciencesV. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of SciencesV. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of SciencesDuring deep technological processing, DNA of food product components (specifically, in canned foods) is subjected to strong degradation, which makes the PCR-based food components identification more difficult. In this work, a primer-probe system is proposed, which was selected for the multi-copy region of long terminal repeat (LTR) of soybean (Glycine max). We confirmed its high sensitivity and specificity for soybean detection by real-time PCR. Using the selected system, we successfully analyzed the samples of meat-and-plant canned foods and other food products subjected to deep technological processing — tofu, preserved tofu, soy sauces, confectionary products containing soy lecithin. To compare with these samples, real-time PCR was carried out using the primer-probe system selected for the single-copy le1 gene. In terms of sensitivity, the use of the primer-probe system specific to the single-copy region was significantly inferior to the primer-probe system specific to the LTR region. The difference in the rate of degradation of these genomic DNA regions of Glycine max was found.https://www.meatjournal.ru/jour/article/view/126real-time pcrglycine maxspecies identificationfood processingdna degradation |
| spellingShingle | K. A. Kurbakov E. A. Konorov V. N. Zhulinkova M. Yu. Minaev Detection of soybean by real-time PCR in the samples subjected to deep technological processing Теория и практика переработки мяса real-time pcr glycine max species identification food processing dna degradation |
| title | Detection of soybean by real-time PCR in the samples subjected to deep technological processing |
| title_full | Detection of soybean by real-time PCR in the samples subjected to deep technological processing |
| title_fullStr | Detection of soybean by real-time PCR in the samples subjected to deep technological processing |
| title_full_unstemmed | Detection of soybean by real-time PCR in the samples subjected to deep technological processing |
| title_short | Detection of soybean by real-time PCR in the samples subjected to deep technological processing |
| title_sort | detection of soybean by real time pcr in the samples subjected to deep technological processing |
| topic | real-time pcr glycine max species identification food processing dna degradation |
| url | https://www.meatjournal.ru/jour/article/view/126 |
| work_keys_str_mv | AT kakurbakov detectionofsoybeanbyrealtimepcrinthesamplessubjectedtodeeptechnologicalprocessing AT eakonorov detectionofsoybeanbyrealtimepcrinthesamplessubjectedtodeeptechnologicalprocessing AT vnzhulinkova detectionofsoybeanbyrealtimepcrinthesamplessubjectedtodeeptechnologicalprocessing AT myuminaev detectionofsoybeanbyrealtimepcrinthesamplessubjectedtodeeptechnologicalprocessing |