Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells
In order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited. This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human r...
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| Format: | Article |
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Tsinghua University Press
2024-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250138 |
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| author | Zhuqing Dai Meimei Nie Ye Chen Jiangfeng Song Yayuan Xu Zhongyuan Zhang Guodong Zhang Shumo Yan Xing Zhang Dajing Li |
| author_facet | Zhuqing Dai Meimei Nie Ye Chen Jiangfeng Song Yayuan Xu Zhongyuan Zhang Guodong Zhang Shumo Yan Xing Zhang Dajing Li |
| author_sort | Zhuqing Dai |
| collection | DOAJ |
| description | In order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited. This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial (ARPE) cells. LUT and LUT-STE (final concentration of 5 μg/mL) significantly enhanced cell viability from (74.84 ± 5.10)% to (81.92 ± 10.01)% (LUT) and (89.33 ± 4.34)% (LUT-STE), and inhibited the cell apoptosis (P < 0.05). After pretreatment with LUT-STE in ARPE cells, the levels of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in ARPE cells were significantly increased (P < 0.05), the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased. In addition, the vascular endothelial growth factor (VEGF) levels were inhibited by 13.61% and 17.39%, respectively, pretreatment with LUT and LUT-STE. Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis. In summary, the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells. |
| format | Article |
| id | doaj-art-aa462f90c22344b6a100227a0c5191d1 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-aa462f90c22344b6a100227a0c5191d12025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331628163510.26599/FSHW.2022.9250138Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cellsZhuqing Dai0Meimei Nie1Ye Chen2Jiangfeng Song3Yayuan Xu4Zhongyuan Zhang5Guodong Zhang6Shumo Yan7Xing Zhang8Dajing Li9Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Aland Nutrition Co., Ltd., Taizhou 214500, ChinaAland Nutrition Taizhou Co., Ltd., Taizhou 225300, ChinaJiangsu Aland Nutrition Co., Ltd., Taizhou 214500, ChinaInstitute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaIn order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited. This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial (ARPE) cells. LUT and LUT-STE (final concentration of 5 μg/mL) significantly enhanced cell viability from (74.84 ± 5.10)% to (81.92 ± 10.01)% (LUT) and (89.33 ± 4.34)% (LUT-STE), and inhibited the cell apoptosis (P < 0.05). After pretreatment with LUT-STE in ARPE cells, the levels of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in ARPE cells were significantly increased (P < 0.05), the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased. In addition, the vascular endothelial growth factor (VEGF) levels were inhibited by 13.61% and 17.39%, respectively, pretreatment with LUT and LUT-STE. Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis. In summary, the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells.https://www.sciopen.com/article/10.26599/FSHW.2022.9250138luteinsteviosideantioxidanthuman retinal pigment epithelial cellmechanism |
| spellingShingle | Zhuqing Dai Meimei Nie Ye Chen Jiangfeng Song Yayuan Xu Zhongyuan Zhang Guodong Zhang Shumo Yan Xing Zhang Dajing Li Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells Food Science and Human Wellness lutein stevioside antioxidant human retinal pigment epithelial cell mechanism |
| title | Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells |
| title_full | Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells |
| title_fullStr | Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells |
| title_full_unstemmed | Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells |
| title_short | Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells |
| title_sort | lutein stevioside nanoparticle attenuates h2o2 induced oxidative damage in arpe cells |
| topic | lutein stevioside antioxidant human retinal pigment epithelial cell mechanism |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250138 |
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