Level of involvement of four selected cytochrome P450s (CYPs) in pyrethroid-resistant Anopheles gambiae (s.s.) and Anopheles coluzzii across Côte d’Ivoire
In Côte d'Ivoire, there is a high intensity of pyrethroid resistance in malaria vectors which may threaten successful vector control. Molecular studies of the target site mutation in the voltage-gated sodium channel (VGSC) 1014F show that the gene frequencies of these mutations are high, widely...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Current Research in Parasitology and Vector-Borne Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667114X24000542 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846148759092723712 |
|---|---|
| author | Joseph Chabi Constant V.A. Edi Bernard L. Kouassi Constant N.G. Gbalegba Armand E. Kouassi Jackson K.I. Kouame Yves K.A. Kadio Firmain N. Yokoly Louise B. Golou Janice Gouaméné Jean-Baptiste Assamoi Emmanuel Tia Roseline M. Yapo Lucien Y. Konan Roméo N. N’Tamon Alphonsine A. Koffi Antoine M. Tanoh Ndombour Ging-Cissé Pascal Zinzindohoué Blaise Kouadio Patricia L. Yepassis-Zembrou Seth R. Irish Cecilia Flatley Matthew Kirby |
| author_facet | Joseph Chabi Constant V.A. Edi Bernard L. Kouassi Constant N.G. Gbalegba Armand E. Kouassi Jackson K.I. Kouame Yves K.A. Kadio Firmain N. Yokoly Louise B. Golou Janice Gouaméné Jean-Baptiste Assamoi Emmanuel Tia Roseline M. Yapo Lucien Y. Konan Roméo N. N’Tamon Alphonsine A. Koffi Antoine M. Tanoh Ndombour Ging-Cissé Pascal Zinzindohoué Blaise Kouadio Patricia L. Yepassis-Zembrou Seth R. Irish Cecilia Flatley Matthew Kirby |
| author_sort | Joseph Chabi |
| collection | DOAJ |
| description | In Côte d'Ivoire, there is a high intensity of pyrethroid resistance in malaria vectors which may threaten successful vector control. Molecular studies of the target site mutation in the voltage-gated sodium channel (VGSC) 1014F show that the gene frequencies of these mutations are high, widely spread across Côte d’Ivoire, and even fixed in some areas. To further characterize insecticide resistance in Côte d’Ivoire beyond target site mutations, the metabolic resistance mechanism was explored. Overexpression of the metabolic enzyme cytochrome CYP6M2, CYP6P3, CYP6P4, and CYP6P5 was investigated in Anopheles gambiae (s.s.) and An. coluzzii collected from 30 insecticide resistance monitoring sites across the country in 2021 and 2022. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was carried out using RNA-later preserved 60 surviving mosquitoes from each site after phenotypic susceptibility tests. Additionally, a subsample of about 100 mosquitoes from each site was identified to species by PCR, and the frequency of the VGSC 1014F and 1014S was determined. All four CYPs were found to be overexpressed in Côte d’Ivoire with at least one CYP statistically significantly overexpressed in 27 of the 30 sites investigated compared to the susceptible An. gambiae (s.s.) Kisumu (P < 0.005). CYP6M2 was overexpressed in 89% of sites and was the sole overexpressed gene in 10 sites, while the overexpression of CYP6P3 was found in only 10 sites. CYP6P4 and CYP6P5 were overexpressed in 16 and 13 sites, respectively. Furthermore, seven sites (Adzopé, Bongouanou, Daloa, Gagnoa, Guiglo Jacqueville, and Sassandra) had overexpression of all four CYPs. Overall, An. coluzzii showed higher overexpression of CYPs than An. gambiae (s.s.). This study highlights the involvement of selected CYPs in insecticide resistance where target site mutation genes are already present, suggesting that insecticide resistance is complex and multifaceted at a molecular level. Where feasible, it may be helpful to include metabolic resistance surveillance to further characterize insecticide resistance. |
| format | Article |
| id | doaj-art-aa38d43e7e5e436c9fc04756f9649927 |
| institution | Kabale University |
| issn | 2667-114X |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Parasitology and Vector-Borne Diseases |
| spelling | doaj-art-aa38d43e7e5e436c9fc04756f96499272024-11-30T07:14:33ZengElsevierCurrent Research in Parasitology and Vector-Borne Diseases2667-114X2024-01-016100223Level of involvement of four selected cytochrome P450s (CYPs) in pyrethroid-resistant Anopheles gambiae (s.s.) and Anopheles coluzzii across Côte d’IvoireJoseph Chabi0Constant V.A. Edi1Bernard L. Kouassi2Constant N.G. Gbalegba3Armand E. Kouassi4Jackson K.I. Kouame5Yves K.A. Kadio6Firmain N. Yokoly7Louise B. Golou8Janice Gouaméné9Jean-Baptiste Assamoi10Emmanuel Tia11Roseline M. Yapo12Lucien Y. Konan13Roméo N. N’Tamon14Alphonsine A. Koffi15Antoine M. Tanoh16Ndombour Ging-Cissé17Pascal Zinzindohoué18Blaise Kouadio19Patricia L. Yepassis-Zembrou20Seth R. Irish21Cecilia Flatley22Matthew Kirby23U.S. President’s Malaria Initiative, Evolve Project, Washington DC, USA; Corresponding author.Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Cote d’IvoireU.S. President’s Malaria Initiative, Evolve Project, Abidjan, Cote d’IvoireNational Malaria Control Programme, Abidjan, Cote d’IvoireCentre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Cote d’IvoireCentre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Cote d’IvoireCentre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Cote d’IvoireCentre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Cote d’IvoireCentre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Cote d’IvoireCentre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Cote d’IvoireCentre Suisse de Recherches Scientifiques en Côte d’Ivoire, Abidjan, Cote d’IvoireUniversité Alassane Ouattara, Centre d’Entomologie Médicale et Vétérinaire, Abidjan, Cote d’IvoireUniversité Alassane Ouattara, Centre d’Entomologie Médicale et Vétérinaire, Abidjan, Cote d’IvoireInstitut National d’Hygiène Publique, Abidjan, Cote d’IvoireInstitut National d’Hygiène Publique, Abidjan, Cote d’IvoireInstitut National de Sante Publique/Institut Pierre Richet, Bouake, Cote d’IvoireNational Malaria Control Programme, Abidjan, Cote d’IvoireU.S. President’s Malaria Initiative, Evolve Project, Abidjan, Cote d’IvoireU.S. President’s Malaria Initiative, USAID, Abidjan, Cote d’IvoireU.S. President’s Malaria Initiative, USAID, Abidjan, Cote d’IvoireU.S. President’s Malaria Initiative, U.S. Centers for Disease Control and Prevention, Abidjan, Cote d’IvoireU.S. President’s Malaria Initiative, Malaria/Entomology Branch, U.S. Centers for Disease Control and Prevention, Atlanta, GA, USAU.S. President’s Malaria Initiative, Evolve Project, Washington DC, USAU.S. President’s Malaria Initiative, Evolve Project, Washington DC, USAIn Côte d'Ivoire, there is a high intensity of pyrethroid resistance in malaria vectors which may threaten successful vector control. Molecular studies of the target site mutation in the voltage-gated sodium channel (VGSC) 1014F show that the gene frequencies of these mutations are high, widely spread across Côte d’Ivoire, and even fixed in some areas. To further characterize insecticide resistance in Côte d’Ivoire beyond target site mutations, the metabolic resistance mechanism was explored. Overexpression of the metabolic enzyme cytochrome CYP6M2, CYP6P3, CYP6P4, and CYP6P5 was investigated in Anopheles gambiae (s.s.) and An. coluzzii collected from 30 insecticide resistance monitoring sites across the country in 2021 and 2022. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was carried out using RNA-later preserved 60 surviving mosquitoes from each site after phenotypic susceptibility tests. Additionally, a subsample of about 100 mosquitoes from each site was identified to species by PCR, and the frequency of the VGSC 1014F and 1014S was determined. All four CYPs were found to be overexpressed in Côte d’Ivoire with at least one CYP statistically significantly overexpressed in 27 of the 30 sites investigated compared to the susceptible An. gambiae (s.s.) Kisumu (P < 0.005). CYP6M2 was overexpressed in 89% of sites and was the sole overexpressed gene in 10 sites, while the overexpression of CYP6P3 was found in only 10 sites. CYP6P4 and CYP6P5 were overexpressed in 16 and 13 sites, respectively. Furthermore, seven sites (Adzopé, Bongouanou, Daloa, Gagnoa, Guiglo Jacqueville, and Sassandra) had overexpression of all four CYPs. Overall, An. coluzzii showed higher overexpression of CYPs than An. gambiae (s.s.). This study highlights the involvement of selected CYPs in insecticide resistance where target site mutation genes are already present, suggesting that insecticide resistance is complex and multifaceted at a molecular level. Where feasible, it may be helpful to include metabolic resistance surveillance to further characterize insecticide resistance.http://www.sciencedirect.com/science/article/pii/S2667114X24000542Insecticide resistanceAnopheles gambiae (s.s.) Anopheles coluzziiCYPMalariaVector control |
| spellingShingle | Joseph Chabi Constant V.A. Edi Bernard L. Kouassi Constant N.G. Gbalegba Armand E. Kouassi Jackson K.I. Kouame Yves K.A. Kadio Firmain N. Yokoly Louise B. Golou Janice Gouaméné Jean-Baptiste Assamoi Emmanuel Tia Roseline M. Yapo Lucien Y. Konan Roméo N. N’Tamon Alphonsine A. Koffi Antoine M. Tanoh Ndombour Ging-Cissé Pascal Zinzindohoué Blaise Kouadio Patricia L. Yepassis-Zembrou Seth R. Irish Cecilia Flatley Matthew Kirby Level of involvement of four selected cytochrome P450s (CYPs) in pyrethroid-resistant Anopheles gambiae (s.s.) and Anopheles coluzzii across Côte d’Ivoire Current Research in Parasitology and Vector-Borne Diseases Insecticide resistance Anopheles gambiae (s.s.) Anopheles coluzzii CYP Malaria Vector control |
| title | Level of involvement of four selected cytochrome P450s (CYPs) in pyrethroid-resistant Anopheles gambiae (s.s.) and Anopheles coluzzii across Côte d’Ivoire |
| title_full | Level of involvement of four selected cytochrome P450s (CYPs) in pyrethroid-resistant Anopheles gambiae (s.s.) and Anopheles coluzzii across Côte d’Ivoire |
| title_fullStr | Level of involvement of four selected cytochrome P450s (CYPs) in pyrethroid-resistant Anopheles gambiae (s.s.) and Anopheles coluzzii across Côte d’Ivoire |
| title_full_unstemmed | Level of involvement of four selected cytochrome P450s (CYPs) in pyrethroid-resistant Anopheles gambiae (s.s.) and Anopheles coluzzii across Côte d’Ivoire |
| title_short | Level of involvement of four selected cytochrome P450s (CYPs) in pyrethroid-resistant Anopheles gambiae (s.s.) and Anopheles coluzzii across Côte d’Ivoire |
| title_sort | level of involvement of four selected cytochrome p450s cyps in pyrethroid resistant anopheles gambiae s s and anopheles coluzzii across cote d ivoire |
| topic | Insecticide resistance Anopheles gambiae (s.s.) Anopheles coluzzii CYP Malaria Vector control |
| url | http://www.sciencedirect.com/science/article/pii/S2667114X24000542 |
| work_keys_str_mv | AT josephchabi levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT constantvaedi levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT bernardlkouassi levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT constantnggbalegba levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT armandekouassi levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT jacksonkikouame levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT yveskakadio levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT firmainnyokoly levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT louisebgolou levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT janicegouamene levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT jeanbaptisteassamoi levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT emmanueltia levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT roselinemyapo levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT lucienykonan levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT romeonntamon levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT alphonsineakoffi levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT antoinemtanoh levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT ndombourgingcisse levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT pascalzinzindohoue levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT blaisekouadio levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT patricialyepassiszembrou levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT sethrirish levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT ceciliaflatley levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire AT matthewkirby levelofinvolvementoffourselectedcytochromep450scypsinpyrethroidresistantanophelesgambiaessandanophelescoluzziiacrosscotedivoire |