Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization

Underwater exercise is becoming increasingly prevalent, during which brain function is necessary but is also at risk. However, no study has explored how prolonged exercise affect the brain in underwater environment. Previous studies have indicated that excessive exercise in common environment causes...

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Main Authors: Houyu Zhao, Kun Liang, Zeyuan Yu, Yukun Wen, Jin Shi, Tingting Zhang, Xuhua Yu, Xianpeng Zu, Yiqun Fang
Format: Article
Language:English
Published: Elsevier 2024-10-01
Series:Neuroscience Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S0168010224000725
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author Houyu Zhao
Kun Liang
Zeyuan Yu
Yukun Wen
Jin Shi
Tingting Zhang
Xuhua Yu
Xianpeng Zu
Yiqun Fang
author_facet Houyu Zhao
Kun Liang
Zeyuan Yu
Yukun Wen
Jin Shi
Tingting Zhang
Xuhua Yu
Xianpeng Zu
Yiqun Fang
author_sort Houyu Zhao
collection DOAJ
description Underwater exercise is becoming increasingly prevalent, during which brain function is necessary but is also at risk. However, no study has explored how prolonged exercise affect the brain in underwater environment. Previous studies have indicated that excessive exercise in common environment causes brain dysfunction but have failed to provide appropriate interventions. Numerous evidence has indicated the neuroprotective effect of hyperbaric oxygen preconditioning (HBO-PC). The objective of this study was to investigate the cognitive effect of prolonged underwater exercise (PUE) and to explore the potential neuroprotective effect of HBO-PC in underwater environment. Rats swimming for 3 h in a simulated hyperbaric chamber (2.0 ATA) was used to establish the PUE animal model and HBO-PC (2.5 ATA for 1, 3,5 times respectively) was administrated before PUE. The results demonstrated that PUE triggers anxiety-like behaviors, cognitive impairment accompanied by hippocampal dysfunction, microglia activation and neuroinflammation. Conversely, 3 HBO-PC rescued anxiety-like behaviors and cognitive impairment. Mechanistically, 3 HBO-PC reduced microglia activation and switched the activated microglia from a pro-inflammatory to neuroprotective phenotype. These findings illustrated that PUE induces anxiety-like behaviors and cognitive impairment and HBO-PC of proper frequency may provide an appropriate and less invasive intervention for protecting the brain in underwater exercise.
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spelling doaj-art-aa1ea050e75c4b36ad641bc6e09458312024-11-21T06:02:36ZengElsevierNeuroscience Research0168-01022024-10-012072636Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarizationHouyu Zhao0Kun Liang1Zeyuan Yu2Yukun Wen3Jin Shi4Tingting Zhang5Xuhua Yu6Xianpeng Zu7Yiqun Fang8Department of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, Shanghai 200433, ChinaDepartment of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, Shanghai 200433, China; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200433, ChinaDepartment of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, Shanghai 200433, ChinaDepartment of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, Shanghai 200433, ChinaDepartment of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, Shanghai 200433, ChinaDepartment of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, Shanghai 200433, ChinaDepartment of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, Shanghai 200433, ChinaSchool of Pharmacy, Naval Medical University, Shanghai 200433, China; Corresponding author.Department of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, Shanghai 200433, China; Correspondence to: Department of Diving and Hyperbaric Medicine, Navy Special Medical Center, Naval Medical University, No. 880 Xiangyin Road, Yangpu District, Shanghai 200433, China.Underwater exercise is becoming increasingly prevalent, during which brain function is necessary but is also at risk. However, no study has explored how prolonged exercise affect the brain in underwater environment. Previous studies have indicated that excessive exercise in common environment causes brain dysfunction but have failed to provide appropriate interventions. Numerous evidence has indicated the neuroprotective effect of hyperbaric oxygen preconditioning (HBO-PC). The objective of this study was to investigate the cognitive effect of prolonged underwater exercise (PUE) and to explore the potential neuroprotective effect of HBO-PC in underwater environment. Rats swimming for 3 h in a simulated hyperbaric chamber (2.0 ATA) was used to establish the PUE animal model and HBO-PC (2.5 ATA for 1, 3,5 times respectively) was administrated before PUE. The results demonstrated that PUE triggers anxiety-like behaviors, cognitive impairment accompanied by hippocampal dysfunction, microglia activation and neuroinflammation. Conversely, 3 HBO-PC rescued anxiety-like behaviors and cognitive impairment. Mechanistically, 3 HBO-PC reduced microglia activation and switched the activated microglia from a pro-inflammatory to neuroprotective phenotype. These findings illustrated that PUE induces anxiety-like behaviors and cognitive impairment and HBO-PC of proper frequency may provide an appropriate and less invasive intervention for protecting the brain in underwater exercise.http://www.sciencedirect.com/science/article/pii/S0168010224000725Prolonged underwater exerciseCognitive impairmentHyperbaric oxygen preconditioningNeuroinflammationMicroglia
spellingShingle Houyu Zhao
Kun Liang
Zeyuan Yu
Yukun Wen
Jin Shi
Tingting Zhang
Xuhua Yu
Xianpeng Zu
Yiqun Fang
Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization
Neuroscience Research
Prolonged underwater exercise
Cognitive impairment
Hyperbaric oxygen preconditioning
Neuroinflammation
Microglia
title Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization
title_full Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization
title_fullStr Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization
title_full_unstemmed Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization
title_short Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization
title_sort hyperbaric oxygen preconditioning rescues prolonged underwater exercise induced hippocampal dysfunction by regulating microglia activation and polarization
topic Prolonged underwater exercise
Cognitive impairment
Hyperbaric oxygen preconditioning
Neuroinflammation
Microglia
url http://www.sciencedirect.com/science/article/pii/S0168010224000725
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