Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, China
AIM: To isolate and identify the conjunctival microbiota of cataract patients and analyze the associated influencing factors. METHODS: This study recruited 216 participants (216 eyes) from April 2022 to July 2022. Under the condition of no antibiotic use prior to cataract surgery, sterile swabs were...
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Press of International Journal of Ophthalmology (IJO PRESS)
2025-02-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2025/2/20250206.pdf |
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| author | Tian-Yao Zhao Yu-Chen Wang Ying-Juan Sun Jun-Yue Wang Xiao-Dan Jiang Xue-Min Li Ai-Hua Deng |
| author_facet | Tian-Yao Zhao Yu-Chen Wang Ying-Juan Sun Jun-Yue Wang Xiao-Dan Jiang Xue-Min Li Ai-Hua Deng |
| author_sort | Tian-Yao Zhao |
| collection | DOAJ |
| description | AIM: To isolate and identify the conjunctival microbiota of cataract patients and analyze the associated influencing factors. METHODS: This study recruited 216 participants (216 eyes) from April 2022 to July 2022. Under the condition of no antibiotic use prior to cataract surgery, sterile swabs were used to collect samples from the lower conjunctival sac. Bacterial cultures were then conducted, followed by species identification through 16S rDNA gene sequencing. Clinical factors associated with positive or negative bacterial isolation rates were analyzed, including age, gender, meibomian gland dysfunction (MGD), history of hypertension, history of diabetes, history of cancer, history of infectious diseases and the habit of wearing masks. RESULTS: Among the 216 eyes, 78 eyes yielded isolates, with an isolation rate of 36.11%, detecting a total of 122 strains. Gram-positive rods accounted for 49.18% (60 strains), gram-positive cocci accounted for 45.08% (55 strains), gram-negative bacteria accounted for 4.92% (6 strains), and fungi accounted for 0.82% (1 strain). This study found that the most abundant genera in the conjunctival sac were Corynebacterium (42.62%), Staphylococcus (31.15%), Micrococcus (9.84%), Acinetobacter (4.10%), and Bacillus (3.28%). Furthermore, age (P=0.006), gender (P=0.039), diabetes (P=0.003), history of infectious diseases (P=0.02), and duration of mask replacement (P<0.001) were important factors influencing the positive bacterial culture of the conjunctival microbiota. Although hypertensive patients exhibited a higher isolation rate of conjunctival bacteria, it did not reach statistical significance, and the history of cancer did not affect the isolation rate of the conjunctival microbial community in cataract patients before surgery. CONCLUSION: Potential changes are observed in the conjunctival microbiota among a sample of middle-aged and elderly individuals from Beijing, China. Notably, an increased isolation rate of Corynebacterium and Micrococcus is detected, suggesting a possible change in the microbial balance that requires further investigation and attention from the ophthalmological community. Advanced age, female gender, MGD, diabetes, a recent history of infectious diseases, and inadequate mask-wearing habits are potentially significant factors associated with the conjunctival microbiota. These factors should be considered in the development of strategies to prevent perioperative infections in cataract surgery patients. |
| format | Article |
| id | doaj-art-a9f066828565476ebac5cb62883ba6d4 |
| institution | Kabale University |
| issn | 2222-3959 2227-4898 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj-art-a9f066828565476ebac5cb62883ba6d42025-01-16T07:54:35ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982025-02-0118223724310.18240/ijo.2025.02.0620250206Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, ChinaTian-Yao Zhao0Yu-Chen Wang1Ying-Juan Sun2Jun-Yue Wang3Xiao-Dan Jiang4Xue-Min Li5Ai-Hua Deng6Xue-Min Li. Department of Ophthalmology, Peking University Third Hospital, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, 49 North Garden Road, Haidian District, Beijing 100191, China. Ai-Hua Deng. State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture and Rural Affairs Feed Industry Centre, Agricultural University, Beijing 100193, China. lxmlxm66@sina.com; dengah@cau.edu.cnDepartment of Ophthalmology, Peking University Third Hospital, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Beijing 100191, ChinaFangshan Hospital Beijing University of Chinese Medicine, Beijing 102400, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Beijing 100191, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Beijing 100191, ChinaState Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture and Rural Affairs Feed Industry Centre, Agricultural University, Beijing 100193, ChinaAIM: To isolate and identify the conjunctival microbiota of cataract patients and analyze the associated influencing factors. METHODS: This study recruited 216 participants (216 eyes) from April 2022 to July 2022. Under the condition of no antibiotic use prior to cataract surgery, sterile swabs were used to collect samples from the lower conjunctival sac. Bacterial cultures were then conducted, followed by species identification through 16S rDNA gene sequencing. Clinical factors associated with positive or negative bacterial isolation rates were analyzed, including age, gender, meibomian gland dysfunction (MGD), history of hypertension, history of diabetes, history of cancer, history of infectious diseases and the habit of wearing masks. RESULTS: Among the 216 eyes, 78 eyes yielded isolates, with an isolation rate of 36.11%, detecting a total of 122 strains. Gram-positive rods accounted for 49.18% (60 strains), gram-positive cocci accounted for 45.08% (55 strains), gram-negative bacteria accounted for 4.92% (6 strains), and fungi accounted for 0.82% (1 strain). This study found that the most abundant genera in the conjunctival sac were Corynebacterium (42.62%), Staphylococcus (31.15%), Micrococcus (9.84%), Acinetobacter (4.10%), and Bacillus (3.28%). Furthermore, age (P=0.006), gender (P=0.039), diabetes (P=0.003), history of infectious diseases (P=0.02), and duration of mask replacement (P<0.001) were important factors influencing the positive bacterial culture of the conjunctival microbiota. Although hypertensive patients exhibited a higher isolation rate of conjunctival bacteria, it did not reach statistical significance, and the history of cancer did not affect the isolation rate of the conjunctival microbial community in cataract patients before surgery. CONCLUSION: Potential changes are observed in the conjunctival microbiota among a sample of middle-aged and elderly individuals from Beijing, China. Notably, an increased isolation rate of Corynebacterium and Micrococcus is detected, suggesting a possible change in the microbial balance that requires further investigation and attention from the ophthalmological community. Advanced age, female gender, MGD, diabetes, a recent history of infectious diseases, and inadequate mask-wearing habits are potentially significant factors associated with the conjunctival microbiota. These factors should be considered in the development of strategies to prevent perioperative infections in cataract surgery patients.http://ies.ijo.cn/en_publish/2025/2/20250206.pdfconjunctival microbiota16s rdnamiddle-aged and elderly populationmaskcataract surgery |
| spellingShingle | Tian-Yao Zhao Yu-Chen Wang Ying-Juan Sun Jun-Yue Wang Xiao-Dan Jiang Xue-Min Li Ai-Hua Deng Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, China International Journal of Ophthalmology conjunctival microbiota 16s rdna middle-aged and elderly population mask cataract surgery |
| title | Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, China |
| title_full | Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, China |
| title_fullStr | Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, China |
| title_full_unstemmed | Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, China |
| title_short | Conjunctival microbiota variations in a subset of middle-aged and elderly individuals from Beijing, China |
| title_sort | conjunctival microbiota variations in a subset of middle aged and elderly individuals from beijing china |
| topic | conjunctival microbiota 16s rdna middle-aged and elderly population mask cataract surgery |
| url | http://ies.ijo.cn/en_publish/2025/2/20250206.pdf |
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