Poor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in Brucella-seropositive dromedary camel bulls
Abstract Brucellosis in dromedary camel bulls leads to either temporary or permanent loss of fertility. Camel brucellosis is associated with both orchitis and epididymitis. However, the clinical signs of camel brucellosis are not clear as those in cattle. Therefore, this study aimed to diagnose came...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-74018-y |
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| author | Ahmed Saad Ahmed Hassaneen Anis Anis Safaa Y. Nour Rasha Salah Mohamed Islam M. Wassif Adel M. El-kattan Hosny Ahmed Abdelgawad Ragab H. Mohamed |
| author_facet | Ahmed Saad Ahmed Hassaneen Anis Anis Safaa Y. Nour Rasha Salah Mohamed Islam M. Wassif Adel M. El-kattan Hosny Ahmed Abdelgawad Ragab H. Mohamed |
| author_sort | Ahmed Saad Ahmed Hassaneen |
| collection | DOAJ |
| description | Abstract Brucellosis in dromedary camel bulls leads to either temporary or permanent loss of fertility. Camel brucellosis is associated with both orchitis and epididymitis. However, the clinical signs of camel brucellosis are not clear as those in cattle. Therefore, this study aimed to diagnose camel brucellosis based on a serological screening using Rose Bengal plate test (RBPT) followed by competitive ELISA. To understand the impact of brucellosis on camel bull fertility, this study aimed to examine the semen characteristics, evaluate the testicular histopathology, examine hormonal profile, antioxidants and acute phase proteins (APP). A total of 150 mature bulls were used in this study. Blood samples were collected for serological, hormonal, and biochemical analysis. This study revealed that 6.6% and 7.3% of the examined bulls were Brucella-seropositive using RBPT and competitive ELISA, respectively. The Brucella-seropositive dromedary bulls showed poor semen quality, pathological changes orchitis, and lower testosterone. Moreover, our findings showed a higher cortisol level, and significant impairments in the measured APP and antioxidants in Brucella-seropositive bulls. In conclusion, the Brucella-seropositive dromedary bulls showed lower fertility due to poor semen quality and lower testosterone levels. Such lower fertility is likely mediated by high cortisol levels, and impaired APP and antioxidants’ defense response. |
| format | Article |
| id | doaj-art-a9dfde1d87a24b80b24556916c806572 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-a9dfde1d87a24b80b24556916c8065722024-11-17T12:21:53ZengNature PortfolioScientific Reports2045-23222024-11-0114111010.1038/s41598-024-74018-yPoor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in Brucella-seropositive dromedary camel bullsAhmed Saad Ahmed Hassaneen0Anis Anis1Safaa Y. Nour2Rasha Salah Mohamed3Islam M. Wassif4Adel M. El-kattan5Hosny Ahmed Abdelgawad6Ragab H. Mohamed7Department of Theriogenology, Obstetrics, and Artificial Insemination, Faculty of Veterinary Medicine, South Valley UniversityDepartment of Pathology, Faculty of Veterinary Medicine, University of Sadat CityDepartment of Animal Medicine, Faculty of Veterinary Medicine, Aswan UniversityDepartment of Animal and Poultry Health, Desert Research CenterDepartment of Animal and Poultry Health, Desert Research CenterDepartment of Animal and Poultry Health, Desert Research CenterDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, Aswan UniversityDepartment of Theriogenology, Faculty of Veterinary Medicine, Aswan UniversityAbstract Brucellosis in dromedary camel bulls leads to either temporary or permanent loss of fertility. Camel brucellosis is associated with both orchitis and epididymitis. However, the clinical signs of camel brucellosis are not clear as those in cattle. Therefore, this study aimed to diagnose camel brucellosis based on a serological screening using Rose Bengal plate test (RBPT) followed by competitive ELISA. To understand the impact of brucellosis on camel bull fertility, this study aimed to examine the semen characteristics, evaluate the testicular histopathology, examine hormonal profile, antioxidants and acute phase proteins (APP). A total of 150 mature bulls were used in this study. Blood samples were collected for serological, hormonal, and biochemical analysis. This study revealed that 6.6% and 7.3% of the examined bulls were Brucella-seropositive using RBPT and competitive ELISA, respectively. The Brucella-seropositive dromedary bulls showed poor semen quality, pathological changes orchitis, and lower testosterone. Moreover, our findings showed a higher cortisol level, and significant impairments in the measured APP and antioxidants in Brucella-seropositive bulls. In conclusion, the Brucella-seropositive dromedary bulls showed lower fertility due to poor semen quality and lower testosterone levels. Such lower fertility is likely mediated by high cortisol levels, and impaired APP and antioxidants’ defense response.https://doi.org/10.1038/s41598-024-74018-yAntioxidantsBrucellosisCamels’ infectious disordersDromedary bulls infertility |
| spellingShingle | Ahmed Saad Ahmed Hassaneen Anis Anis Safaa Y. Nour Rasha Salah Mohamed Islam M. Wassif Adel M. El-kattan Hosny Ahmed Abdelgawad Ragab H. Mohamed Poor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in Brucella-seropositive dromedary camel bulls Scientific Reports Antioxidants Brucellosis Camels’ infectious disorders Dromedary bulls infertility |
| title | Poor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in Brucella-seropositive dromedary camel bulls |
| title_full | Poor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in Brucella-seropositive dromedary camel bulls |
| title_fullStr | Poor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in Brucella-seropositive dromedary camel bulls |
| title_full_unstemmed | Poor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in Brucella-seropositive dromedary camel bulls |
| title_short | Poor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in Brucella-seropositive dromedary camel bulls |
| title_sort | poor semen quality is associated with impaired antioxidant response and acute phase proteins and is likely mediated by high cortisol levels in brucella seropositive dromedary camel bulls |
| topic | Antioxidants Brucellosis Camels’ infectious disorders Dromedary bulls infertility |
| url | https://doi.org/10.1038/s41598-024-74018-y |
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