Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healing
IntroductionMontrichardia linifera (Arruda) Schott (M. linifera) is commonly used by Amazonian riverine communities for the treatment of skin ulcers, although its effects as a wound healer have never been evaluated until now. Therefore, the in vitro wound-healing activity of the extracts from the st...
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Frontiers Media S.A.
2025-05-01
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| author | Aline Costa Bastos Aline Costa Bastos Maurício Ferreira Gomes Maurício Ferreira Gomes W. B. S. Pinheiro Anderson de Santana Botelho Raimundo Junior da Rocha Batista Cristine Bastos do Amarante Taia Maria Berto Rezende Yasmin Cunha da Silva Ananda da Silva Antonio Henrique Marcelo Gualberto Pereira Valdir Florêncio da Veiga Júnior André Salim Khayat André Salim Khayat Elizabeth S. Yamada Gilmara de Nazareth Tavares Bastos Gilmara de Nazareth Tavares Bastos |
| author_facet | Aline Costa Bastos Aline Costa Bastos Maurício Ferreira Gomes Maurício Ferreira Gomes W. B. S. Pinheiro Anderson de Santana Botelho Raimundo Junior da Rocha Batista Cristine Bastos do Amarante Taia Maria Berto Rezende Yasmin Cunha da Silva Ananda da Silva Antonio Henrique Marcelo Gualberto Pereira Valdir Florêncio da Veiga Júnior André Salim Khayat André Salim Khayat Elizabeth S. Yamada Gilmara de Nazareth Tavares Bastos Gilmara de Nazareth Tavares Bastos |
| author_sort | Aline Costa Bastos |
| collection | DOAJ |
| description | IntroductionMontrichardia linifera (Arruda) Schott (M. linifera) is commonly used by Amazonian riverine communities for the treatment of skin ulcers, although its effects as a wound healer have never been evaluated until now. Therefore, the in vitro wound-healing activity of the extracts from the stem and petiole of M. linifera was investigated for the first time.MethodsThe extracts were characterized by chromatography coupled with spectroscopic or spectrometric methods (HPTLC-UV and UHPLC-MS), and free radical scavenging was verified using bioautography with the DPPH radical. Cytotoxicity was evaluated through the MTT method, and a scratch assay was employed to assess cell migration, while in vitro cell proliferation was evaluated through immunofluorescence for BrdU-positive cells.ResultsChemical characterization revealed the presence of 13 metabolites in ESML and EPML extracts. Analytical analysis of the extract demonstrated the elimination of free radicals by autobiography. The extracts did not demonstrate cytotoxicity in fibroblasts and cell migration and proliferation were, significantly, increased reducing the wound area in vitro.ConclusionThus, it was observed that the extracts from the stem and petiole of M. linifera possess potential wound-healing effects in fibroblasts in vitro. This is a pioneering study that provides insights for future studies on the mechanisms of action of this species, in addition to validating the ethnopharmacological knowledge of this species used in the Amazon. |
| format | Article |
| id | doaj-art-a9ca3ae6b4b74b11a855ecbf71d0e8e3 |
| institution | DOAJ |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-a9ca3ae6b4b74b11a855ecbf71d0e8e32025-08-20T03:07:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-05-011610.3389/fphar.2025.15125701512570Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healingAline Costa Bastos0Aline Costa Bastos1Maurício Ferreira Gomes2Maurício Ferreira Gomes3W. B. S. Pinheiro4Anderson de Santana Botelho5Raimundo Junior da Rocha Batista6Cristine Bastos do Amarante7Taia Maria Berto Rezende8Yasmin Cunha da Silva9Ananda da Silva Antonio10Henrique Marcelo Gualberto Pereira11Valdir Florêncio da Veiga Júnior12André Salim Khayat13André Salim Khayat14Elizabeth S. Yamada15Gilmara de Nazareth Tavares Bastos16Gilmara de Nazareth Tavares Bastos17Laboratory of Neuroinflammation, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilOncology Research Center, Federal University of Pará, Belém, BrazilLaboratory of Neuroinflammation, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilOncology Research Center, Federal University of Pará, Belém, BrazilLaboratory of Central Extraction, Institute of Exact and Natural Sciences, Federal University of Pará, Belém, BrazilLaboratory of Chemical Analysis, Coordination of Earth Sciences and Ecology, Emílio Goeldi Museum, Belém, BrazilLaboratory of Chemical Analysis, Coordination of Earth Sciences and Ecology, Emílio Goeldi Museum, Belém, BrazilLaboratory of Chemical Analysis, Coordination of Earth Sciences and Ecology, Emílio Goeldi Museum, Belém, BrazilFaculty of Health Sciences, University of Brasilia, Brasilia, Federal, BrazilChemical Engineering Section, Military Institute of Engineering, Rio deJaneiro, BrazilLaboratory for the Support of Technological Development, Chemistry Institute, Federal University of Rio de Janeiro, Rio deJaneiro, BrazilLaboratory for the Support of Technological Development, Chemistry Institute, Federal University of Rio de Janeiro, Rio deJaneiro, BrazilChemical Engineering Section, Military Institute of Engineering, Rio deJaneiro, BrazilOncology Research Center, Federal University of Pará, Belém, BrazilInstitute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropathology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Neuroinflammation, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil0Laboratory of Developmental Biology, Department of Morphology, Federal University of São Paulo, SãoPaulo, BrazilIntroductionMontrichardia linifera (Arruda) Schott (M. linifera) is commonly used by Amazonian riverine communities for the treatment of skin ulcers, although its effects as a wound healer have never been evaluated until now. Therefore, the in vitro wound-healing activity of the extracts from the stem and petiole of M. linifera was investigated for the first time.MethodsThe extracts were characterized by chromatography coupled with spectroscopic or spectrometric methods (HPTLC-UV and UHPLC-MS), and free radical scavenging was verified using bioautography with the DPPH radical. Cytotoxicity was evaluated through the MTT method, and a scratch assay was employed to assess cell migration, while in vitro cell proliferation was evaluated through immunofluorescence for BrdU-positive cells.ResultsChemical characterization revealed the presence of 13 metabolites in ESML and EPML extracts. Analytical analysis of the extract demonstrated the elimination of free radicals by autobiography. The extracts did not demonstrate cytotoxicity in fibroblasts and cell migration and proliferation were, significantly, increased reducing the wound area in vitro.ConclusionThus, it was observed that the extracts from the stem and petiole of M. linifera possess potential wound-healing effects in fibroblasts in vitro. This is a pioneering study that provides insights for future studies on the mechanisms of action of this species, in addition to validating the ethnopharmacological knowledge of this species used in the Amazon.https://www.frontiersin.org/articles/10.3389/fphar.2025.1512570/fullMontrichardia liniferahealingskin injurynatural productwound |
| spellingShingle | Aline Costa Bastos Aline Costa Bastos Maurício Ferreira Gomes Maurício Ferreira Gomes W. B. S. Pinheiro Anderson de Santana Botelho Raimundo Junior da Rocha Batista Cristine Bastos do Amarante Taia Maria Berto Rezende Yasmin Cunha da Silva Ananda da Silva Antonio Henrique Marcelo Gualberto Pereira Valdir Florêncio da Veiga Júnior André Salim Khayat André Salim Khayat Elizabeth S. Yamada Gilmara de Nazareth Tavares Bastos Gilmara de Nazareth Tavares Bastos Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healing Frontiers in Pharmacology Montrichardia linifera healing skin injury natural product wound |
| title | Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healing |
| title_full | Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healing |
| title_fullStr | Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healing |
| title_full_unstemmed | Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healing |
| title_short | Montrichardia linifera (Arruda) Schott promotes accelerated wound healing in vitro: a promising healing |
| title_sort | montrichardia linifera arruda schott promotes accelerated wound healing in vitro a promising healing |
| topic | Montrichardia linifera healing skin injury natural product wound |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1512570/full |
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