Rubber plantation impacts on antibiotic resistance genes in tropical groundwater

Study region: Hainan island, China. Study focus: Uncovering the distribution and mechanisms of antibiotic resistance genes (ARGs) in groundwater is crucial for safeguarding public health and environmental sustainability. Despite this, the influence of land use on tropical groundwater ARG profiles re...

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Main Authors: Guoyu Lan, Rui Sun, Tong Pang, Chuan Yang, Zhixiang Wu
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Journal of Hydrology: Regional Studies
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Online Access:http://www.sciencedirect.com/science/article/pii/S2214581825002836
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author Guoyu Lan
Rui Sun
Tong Pang
Chuan Yang
Zhixiang Wu
author_facet Guoyu Lan
Rui Sun
Tong Pang
Chuan Yang
Zhixiang Wu
author_sort Guoyu Lan
collection DOAJ
description Study region: Hainan island, China. Study focus: Uncovering the distribution and mechanisms of antibiotic resistance genes (ARGs) in groundwater is crucial for safeguarding public health and environmental sustainability. Despite this, the influence of land use on tropical groundwater ARG profiles remains a critical knowledge gap. Our study employs metagenomic sequencing to delve into the composition, diversity, and driving forces behind ARGs in Hainan's tropical rainforests and rubber plantations. New hydrologic insights for the region: Our findings reveal a startling contrast: the total abundance of ARGs in rubber plantations soars above that in tropical rainforests, yet no significant differences emerge in α or β diversity. We pinpoint β-Proteobacteria as the primary carriers of ARGs, with Burkholderiaceae bacterium standing out as the key species-level player. Our analysis further unveils that the bacterial community wields the greatest influence (68.5 %) on ARG abundance, while land use exerts a substantial direct impact (27.3 %) and an indirect influence (24.9 %) by shaping the groundwater environment and microbial ecology. Moreover, mobile genetic elements (MGEs) serve a pivotal indirect role (31.5 %) in ARG abundance, albeit without affecting diversity. These insights lays the groundwork for mitigating the dissemination of resistance genes and their potential harm to human health.
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publishDate 2025-06-01
publisher Elsevier
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series Journal of Hydrology: Regional Studies
spelling doaj-art-a99e8c5a0da1459ebdddcd6ce2cc44f22025-08-20T03:13:11ZengElsevierJournal of Hydrology: Regional Studies2214-58182025-06-015910245810.1016/j.ejrh.2025.102458Rubber plantation impacts on antibiotic resistance genes in tropical groundwaterGuoyu Lan0Rui Sun1Tong Pang2Chuan Yang3Zhixiang Wu4Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou City, Hainan Province 571101, China; Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station, Danzhou City, Hainan Province 571737, China; Corresponding author at: Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou City, Hainan Province 571101, China.Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou City, Hainan Province 571101, China; Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station, Danzhou City, Hainan Province 571737, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou City, Hainan Province 571101, China; School of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, Shandong 266109, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou City, Hainan Province 571101, China; Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station, Danzhou City, Hainan Province 571737, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou City, Hainan Province 571101, China; Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station, Danzhou City, Hainan Province 571737, ChinaStudy region: Hainan island, China. Study focus: Uncovering the distribution and mechanisms of antibiotic resistance genes (ARGs) in groundwater is crucial for safeguarding public health and environmental sustainability. Despite this, the influence of land use on tropical groundwater ARG profiles remains a critical knowledge gap. Our study employs metagenomic sequencing to delve into the composition, diversity, and driving forces behind ARGs in Hainan's tropical rainforests and rubber plantations. New hydrologic insights for the region: Our findings reveal a startling contrast: the total abundance of ARGs in rubber plantations soars above that in tropical rainforests, yet no significant differences emerge in α or β diversity. We pinpoint β-Proteobacteria as the primary carriers of ARGs, with Burkholderiaceae bacterium standing out as the key species-level player. Our analysis further unveils that the bacterial community wields the greatest influence (68.5 %) on ARG abundance, while land use exerts a substantial direct impact (27.3 %) and an indirect influence (24.9 %) by shaping the groundwater environment and microbial ecology. Moreover, mobile genetic elements (MGEs) serve a pivotal indirect role (31.5 %) in ARG abundance, albeit without affecting diversity. These insights lays the groundwork for mitigating the dissemination of resistance genes and their potential harm to human health.http://www.sciencedirect.com/science/article/pii/S2214581825002836GroundwaterAntibiotic resistance genesDiversityMobile genetic elementsRubber plantation
spellingShingle Guoyu Lan
Rui Sun
Tong Pang
Chuan Yang
Zhixiang Wu
Rubber plantation impacts on antibiotic resistance genes in tropical groundwater
Journal of Hydrology: Regional Studies
Groundwater
Antibiotic resistance genes
Diversity
Mobile genetic elements
Rubber plantation
title Rubber plantation impacts on antibiotic resistance genes in tropical groundwater
title_full Rubber plantation impacts on antibiotic resistance genes in tropical groundwater
title_fullStr Rubber plantation impacts on antibiotic resistance genes in tropical groundwater
title_full_unstemmed Rubber plantation impacts on antibiotic resistance genes in tropical groundwater
title_short Rubber plantation impacts on antibiotic resistance genes in tropical groundwater
title_sort rubber plantation impacts on antibiotic resistance genes in tropical groundwater
topic Groundwater
Antibiotic resistance genes
Diversity
Mobile genetic elements
Rubber plantation
url http://www.sciencedirect.com/science/article/pii/S2214581825002836
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AT ruisun rubberplantationimpactsonantibioticresistancegenesintropicalgroundwater
AT tongpang rubberplantationimpactsonantibioticresistancegenesintropicalgroundwater
AT chuanyang rubberplantationimpactsonantibioticresistancegenesintropicalgroundwater
AT zhixiangwu rubberplantationimpactsonantibioticresistancegenesintropicalgroundwater