Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activities

This paper aims to isolate and identify the active fraction present in mace essential oil, as well as to evaluate its antioxidant, antibacterial, and antiaging properties. The chemical composition of the oil extracts and fraction were evaluated using gas chromatography-mass spectroscopy (GC-MS). Ant...

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Main Author: Khairan Khairan
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2024-10-01
Series:Journal of Advanced Pharmaceutical Technology & Research
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Online Access:https://journals.lww.com/japtr/fulltext/2024/15040/isolation_of_the_active_fraction_of_mace.6.aspx
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author Khairan Khairan
author_facet Khairan Khairan
author_sort Khairan Khairan
collection DOAJ
description This paper aims to isolate and identify the active fraction present in mace essential oil, as well as to evaluate its antioxidant, antibacterial, and antiaging properties. The chemical composition of the oil extracts and fraction were evaluated using gas chromatography-mass spectroscopy (GC-MS). Antioxidant and antibacterial activity were assessed using DPPH, ABTS, and Kirby–Bauer-Disc diffusion method. The antiaging activity of the active fractions was determined using Schizosaccharomyces pombe yeast as organism model. The GC-MS analysis revealed that the F3 fraction mainly contains a myristicin compound (34.37%). Results from the DPPH and ABTS assays demonstrated that the F3 fraction exhibited the highest antioxidant activity with half maximal inhibitory concentration values of 8.49 ppm and 8.10 ppm, respectively. Antimicrobial activity measurement confirmed that the F3 fraction displayed the strongest antibacterial activity against Staphylococcusaureus, with the diameter inhibition of 11.34 ± 0.32 ppm. Regarding antiaging activity, it was observed that the F3 fractions extended the lifespan of S. pombe at the concentrations tested. The F3 active fraction demonstrated the highest levels of antioxidant, antimicrobial, and antiaging activities.
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institution Kabale University
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language English
publishDate 2024-10-01
publisher Wolters Kluwer Medknow Publications
record_format Article
series Journal of Advanced Pharmaceutical Technology & Research
spelling doaj-art-a96edb4b5add42c48e8beaeb7e2d7e3c2024-12-15T10:24:52ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research0976-20942024-10-0115427628210.4103/JAPTR.JAPTR_417_23Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activitiesKhairan KhairanThis paper aims to isolate and identify the active fraction present in mace essential oil, as well as to evaluate its antioxidant, antibacterial, and antiaging properties. The chemical composition of the oil extracts and fraction were evaluated using gas chromatography-mass spectroscopy (GC-MS). Antioxidant and antibacterial activity were assessed using DPPH, ABTS, and Kirby–Bauer-Disc diffusion method. The antiaging activity of the active fractions was determined using Schizosaccharomyces pombe yeast as organism model. The GC-MS analysis revealed that the F3 fraction mainly contains a myristicin compound (34.37%). Results from the DPPH and ABTS assays demonstrated that the F3 fraction exhibited the highest antioxidant activity with half maximal inhibitory concentration values of 8.49 ppm and 8.10 ppm, respectively. Antimicrobial activity measurement confirmed that the F3 fraction displayed the strongest antibacterial activity against Staphylococcusaureus, with the diameter inhibition of 11.34 ± 0.32 ppm. Regarding antiaging activity, it was observed that the F3 fractions extended the lifespan of S. pombe at the concentrations tested. The F3 active fraction demonstrated the highest levels of antioxidant, antimicrobial, and antiaging activities.https://journals.lww.com/japtr/fulltext/2024/15040/isolation_of_the_active_fraction_of_mace.6.aspxantiaging; antibacterial; antioxidant; myristica fragrans houtt; mace essential oil
spellingShingle Khairan Khairan
Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activities
Journal of Advanced Pharmaceutical Technology & Research
antiaging; antibacterial; antioxidant; myristica fragrans houtt; mace essential oil
title Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activities
title_full Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activities
title_fullStr Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activities
title_full_unstemmed Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activities
title_short Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activities
title_sort isolation of the active fraction of mace myristica fragrans houtt essential oil and their evaluation of antioxidant antibacterial and antiaging activities
topic antiaging; antibacterial; antioxidant; myristica fragrans houtt; mace essential oil
url https://journals.lww.com/japtr/fulltext/2024/15040/isolation_of_the_active_fraction_of_mace.6.aspx
work_keys_str_mv AT khairankhairan isolationoftheactivefractionofmacemyristicafragranshouttessentialoilandtheirevaluationofantioxidantantibacterialandantiagingactivities