Isolation of the active fraction of mace (Myristica fragrans Houtt) essential oil and their evaluation of antioxidant, antibacterial, and antiaging activities
This paper aims to isolate and identify the active fraction present in mace essential oil, as well as to evaluate its antioxidant, antibacterial, and antiaging properties. The chemical composition of the oil extracts and fraction were evaluated using gas chromatography-mass spectroscopy (GC-MS). Ant...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-10-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | https://journals.lww.com/japtr/fulltext/2024/15040/isolation_of_the_active_fraction_of_mace.6.aspx |
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| Summary: | This paper aims to isolate and identify the active fraction present in mace essential oil, as well as to evaluate its antioxidant, antibacterial, and antiaging properties. The chemical composition of the oil extracts and fraction were evaluated using gas chromatography-mass spectroscopy (GC-MS). Antioxidant and antibacterial activity were assessed using DPPH, ABTS, and Kirby–Bauer-Disc diffusion method. The antiaging activity of the active fractions was determined using Schizosaccharomyces pombe yeast as organism model. The GC-MS analysis revealed that the F3 fraction mainly contains a myristicin compound (34.37%). Results from the DPPH and ABTS assays demonstrated that the F3 fraction exhibited the highest antioxidant activity with half maximal inhibitory concentration values of 8.49 ppm and 8.10 ppm, respectively. Antimicrobial activity measurement confirmed that the F3 fraction displayed the strongest antibacterial activity against Staphylococcusaureus, with the diameter inhibition of 11.34 ± 0.32 ppm. Regarding antiaging activity, it was observed that the F3 fractions extended the lifespan of S. pombe at the concentrations tested. The F3 active fraction demonstrated the highest levels of antioxidant, antimicrobial, and antiaging activities. |
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| ISSN: | 0976-2094 |