A New Enzyme for Biodiesel Production and Food Applications: Lipase of Bacillus megaterium F25 Isolated From an Aquatic Insect Rhantus suturalis
ABSTRACT This study aimed to isolate, purify, and characterize a lipase from the gut symbiont Bacillus megaterium F25 (GenBank accession: MF597792) of the aquatic insect Rhantus suturalis, with a focus on its potential applications in biodiesel and food industries. Under optimized culture conditions...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-12-01
|
| Series: | GCB Bioenergy |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/gcbb.70009 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846151559694516224 |
|---|---|
| author | Fatima Karaman Umit Incekara Nazli Pinar Arslan Seyda Albayrak Serkan Ortucu Mesut Taskin |
| author_facet | Fatima Karaman Umit Incekara Nazli Pinar Arslan Seyda Albayrak Serkan Ortucu Mesut Taskin |
| author_sort | Fatima Karaman |
| collection | DOAJ |
| description | ABSTRACT This study aimed to isolate, purify, and characterize a lipase from the gut symbiont Bacillus megaterium F25 (GenBank accession: MF597792) of the aquatic insect Rhantus suturalis, with a focus on its potential applications in biodiesel and food industries. Under optimized culture conditions, B. megaterium F25 could produce 583 U/L of lipase in shaking flask culture. The purified lipase (PL) exhibited a specific activity with 113.89 U/mg, and its molecular weight was determined as 34 kDa. The activity of PL was enhanced by methanol, ethanol, Tween‐80, Triton X‐100, Ca2+, and Mg2+, while β‐mercaptoethanol, EDTA, SDS, Fe2+, Mn2+, and Cu2+ were inhibitory. PL showed optimal activity and stability at neutral and slightly acidic pHs, as well as in a temperature range of 20°C–30°C. PL displayed strong hydrolytic activity toward plant oils and animal fats, indicating its potency for both the food industry and the remediation of oil‐contaminated environments. When tested as a catalyst, PL provided biodiesel production with a transesterification yield of 86.8% under optimized conditions (36 h reaction time, 4 mL enzyme solution, 30°C, pH 7.0, and waste cooking oil:methanol ratio of 10 mL/40 mL). This is the first report on the lipase‐producing potential of gut microbial symbionts of aquatic insects. Furthermore, B. megaterium lipase was tested for the first time as a biocatalyst for biodiesel production. |
| format | Article |
| id | doaj-art-a96b8fd3d4964f60b74d66240c2e9549 |
| institution | Kabale University |
| issn | 1757-1693 1757-1707 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | GCB Bioenergy |
| spelling | doaj-art-a96b8fd3d4964f60b74d66240c2e95492024-11-27T09:32:31ZengWileyGCB Bioenergy1757-16931757-17072024-12-011612n/an/a10.1111/gcbb.70009A New Enzyme for Biodiesel Production and Food Applications: Lipase of Bacillus megaterium F25 Isolated From an Aquatic Insect Rhantus suturalisFatima Karaman0Umit Incekara1Nazli Pinar Arslan2Seyda Albayrak3Serkan Ortucu4Mesut Taskin5Department of Biology, Science Faculty Ataturk University Erzurum TurkeyDepartment of Biology, Science Faculty Ataturk University Erzurum TurkeyVocational School of Health Services Bingol University Bingol TurkeyDepartment of Molecular Biology and Genetics, Science Faculty Ataturk University Erzurum TurkeyDepartment of Molecular Biology and Genetics, Science Faculty Erzurum Technical University Erzurum TurkeyDepartment of Molecular Biology and Genetics, Science Faculty Ataturk University Erzurum TurkeyABSTRACT This study aimed to isolate, purify, and characterize a lipase from the gut symbiont Bacillus megaterium F25 (GenBank accession: MF597792) of the aquatic insect Rhantus suturalis, with a focus on its potential applications in biodiesel and food industries. Under optimized culture conditions, B. megaterium F25 could produce 583 U/L of lipase in shaking flask culture. The purified lipase (PL) exhibited a specific activity with 113.89 U/mg, and its molecular weight was determined as 34 kDa. The activity of PL was enhanced by methanol, ethanol, Tween‐80, Triton X‐100, Ca2+, and Mg2+, while β‐mercaptoethanol, EDTA, SDS, Fe2+, Mn2+, and Cu2+ were inhibitory. PL showed optimal activity and stability at neutral and slightly acidic pHs, as well as in a temperature range of 20°C–30°C. PL displayed strong hydrolytic activity toward plant oils and animal fats, indicating its potency for both the food industry and the remediation of oil‐contaminated environments. When tested as a catalyst, PL provided biodiesel production with a transesterification yield of 86.8% under optimized conditions (36 h reaction time, 4 mL enzyme solution, 30°C, pH 7.0, and waste cooking oil:methanol ratio of 10 mL/40 mL). This is the first report on the lipase‐producing potential of gut microbial symbionts of aquatic insects. Furthermore, B. megaterium lipase was tested for the first time as a biocatalyst for biodiesel production.https://doi.org/10.1111/gcbb.70009aquatic insectsgut symbiontlipaselipolytic bacteriatransesterification |
| spellingShingle | Fatima Karaman Umit Incekara Nazli Pinar Arslan Seyda Albayrak Serkan Ortucu Mesut Taskin A New Enzyme for Biodiesel Production and Food Applications: Lipase of Bacillus megaterium F25 Isolated From an Aquatic Insect Rhantus suturalis GCB Bioenergy aquatic insects gut symbiont lipase lipolytic bacteria transesterification |
| title | A New Enzyme for Biodiesel Production and Food Applications: Lipase of Bacillus megaterium F25 Isolated From an Aquatic Insect Rhantus suturalis |
| title_full | A New Enzyme for Biodiesel Production and Food Applications: Lipase of Bacillus megaterium F25 Isolated From an Aquatic Insect Rhantus suturalis |
| title_fullStr | A New Enzyme for Biodiesel Production and Food Applications: Lipase of Bacillus megaterium F25 Isolated From an Aquatic Insect Rhantus suturalis |
| title_full_unstemmed | A New Enzyme for Biodiesel Production and Food Applications: Lipase of Bacillus megaterium F25 Isolated From an Aquatic Insect Rhantus suturalis |
| title_short | A New Enzyme for Biodiesel Production and Food Applications: Lipase of Bacillus megaterium F25 Isolated From an Aquatic Insect Rhantus suturalis |
| title_sort | new enzyme for biodiesel production and food applications lipase of bacillus megaterium f25 isolated from an aquatic insect rhantus suturalis |
| topic | aquatic insects gut symbiont lipase lipolytic bacteria transesterification |
| url | https://doi.org/10.1111/gcbb.70009 |
| work_keys_str_mv | AT fatimakaraman anewenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT umitincekara anewenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT nazlipinararslan anewenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT seydaalbayrak anewenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT serkanortucu anewenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT mesuttaskin anewenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT fatimakaraman newenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT umitincekara newenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT nazlipinararslan newenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT seydaalbayrak newenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT serkanortucu newenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis AT mesuttaskin newenzymeforbiodieselproductionandfoodapplicationslipaseofbacillusmegateriumf25isolatedfromanaquaticinsectrhantussuturalis |