Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve Repair
<b>Background:</b> The combination of macroporous cryogels with synthetic peptide factors represents a promising but poorly explored strategy for the development of extracellular matrix (ECM)-mimicking scaffolds for peripheral nerve (PN) repair. <b>Methods:</b> In this study,...
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2024-12-01
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| author | Abdulla Yergeshov Mohamed Zoughaib Kenana Dayob Marat Kamalov Duong Luong Albina Zakirova Ruslan Mullin Diana Salakhieva Timur I. Abdullin |
| author_facet | Abdulla Yergeshov Mohamed Zoughaib Kenana Dayob Marat Kamalov Duong Luong Albina Zakirova Ruslan Mullin Diana Salakhieva Timur I. Abdullin |
| author_sort | Abdulla Yergeshov |
| collection | DOAJ |
| description | <b>Background:</b> The combination of macroporous cryogels with synthetic peptide factors represents a promising but poorly explored strategy for the development of extracellular matrix (ECM)-mimicking scaffolds for peripheral nerve (PN) repair. <b>Methods:</b> In this study, IKVAV peptide was functionalized with terminal lysine residues to allow its in situ cross-linking with gelatin macromer, resulting in the formation of IKVAV-containing proteinaceous cryogels. The controllable inclusion and distribution of the peptide molecules within the scaffold was verified using a fluorescently labelled peptide counterpart. The optimized cryogel scaffold was combined with polycaprolactone (PCL)-based shell tube to form a suturable nerve conduit (NC) to be implanted into sciatic nerve diastasis in rats. <b>Results:</b> The NC constituents did not impair the viability of primary skin fibroblasts. Concentration-dependent effects of the peptide component on interrelated viscoelastic and swelling properties of the cryogels as well as on proliferation and morphological differentiation of neurogenic PC-12 cells were established, also indicating the existence of an optimal-density range of the introduced peptide. The in vivo implanted NC sustained the connection of the nerve stumps with partial degradation of the PCL tube over eight weeks, whereas the core-filling cryogel profoundly improved local electromyographic recovery and morphological repair of the nerve tissues, confirming the regenerative activity of the developed scaffold. <b>Conclusions:</b> These results provide proof-of-concept for the development of a newly designed PN conduit prototype based on IKVAV-activated cryogel, and they can be exploited to create other ECM-mimicking scaffolds. |
| format | Article |
| id | doaj-art-a92d37a5cd124475a99023f8ec95ac4d |
| institution | Kabale University |
| issn | 1999-4923 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-a92d37a5cd124475a99023f8ec95ac4d2024-12-27T14:46:32ZengMDPI AGPharmaceutics1999-49232024-12-011612156910.3390/pharmaceutics16121569Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve RepairAbdulla Yergeshov0Mohamed Zoughaib1Kenana Dayob2Marat Kamalov3Duong Luong4Albina Zakirova5Ruslan Mullin6Diana Salakhieva7Timur I. Abdullin8Scientific and Educational Center of Pharmaceutics, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaScientific and Educational Center of Pharmaceutics, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaScientific and Educational Center of Pharmaceutics, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaScientific and Educational Center of Pharmaceutics, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaInstitute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, AustraliaAcademy of Postgraduate Education under FSBU FSCC of FMBA of Russia, Department of Oncology and Plastic Surgery, 91 Volokolamsk Highway, 125371 Moscow, RussiaInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaScientific and Educational Center of Pharmaceutics, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaScientific and Educational Center of Pharmaceutics, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, Russia<b>Background:</b> The combination of macroporous cryogels with synthetic peptide factors represents a promising but poorly explored strategy for the development of extracellular matrix (ECM)-mimicking scaffolds for peripheral nerve (PN) repair. <b>Methods:</b> In this study, IKVAV peptide was functionalized with terminal lysine residues to allow its in situ cross-linking with gelatin macromer, resulting in the formation of IKVAV-containing proteinaceous cryogels. The controllable inclusion and distribution of the peptide molecules within the scaffold was verified using a fluorescently labelled peptide counterpart. The optimized cryogel scaffold was combined with polycaprolactone (PCL)-based shell tube to form a suturable nerve conduit (NC) to be implanted into sciatic nerve diastasis in rats. <b>Results:</b> The NC constituents did not impair the viability of primary skin fibroblasts. Concentration-dependent effects of the peptide component on interrelated viscoelastic and swelling properties of the cryogels as well as on proliferation and morphological differentiation of neurogenic PC-12 cells were established, also indicating the existence of an optimal-density range of the introduced peptide. The in vivo implanted NC sustained the connection of the nerve stumps with partial degradation of the PCL tube over eight weeks, whereas the core-filling cryogel profoundly improved local electromyographic recovery and morphological repair of the nerve tissues, confirming the regenerative activity of the developed scaffold. <b>Conclusions:</b> These results provide proof-of-concept for the development of a newly designed PN conduit prototype based on IKVAV-activated cryogel, and they can be exploited to create other ECM-mimicking scaffolds.https://www.mdpi.com/1999-4923/16/12/1569peripheral nerve repairnerve conduitscryogelsECM proteinssynthetic peptidesIKVAV derivatives |
| spellingShingle | Abdulla Yergeshov Mohamed Zoughaib Kenana Dayob Marat Kamalov Duong Luong Albina Zakirova Ruslan Mullin Diana Salakhieva Timur I. Abdullin Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve Repair Pharmaceutics peripheral nerve repair nerve conduits cryogels ECM proteins synthetic peptides IKVAV derivatives |
| title | Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve Repair |
| title_full | Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve Repair |
| title_fullStr | Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve Repair |
| title_full_unstemmed | Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve Repair |
| title_short | Newly Designed PCL-Wrapped Cryogel-Based Conduit Activated with IKVAV Peptide Derivative for Peripheral Nerve Repair |
| title_sort | newly designed pcl wrapped cryogel based conduit activated with ikvav peptide derivative for peripheral nerve repair |
| topic | peripheral nerve repair nerve conduits cryogels ECM proteins synthetic peptides IKVAV derivatives |
| url | https://www.mdpi.com/1999-4923/16/12/1569 |
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