Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish
Abstract In vertebrates, skeletal muscle comprises fast and slow fibers. Slow and fast muscle cells in fish are spatially segregated; slow muscle cells are located only in a superficial region, and comprise a small fraction of the total muscle cell mass. Slow muscles support low-speed, low-force mov...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Zoological Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40851-024-00247-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559023153840128 |
|---|---|
| author | Sayaka Shimizu Taisei Katayama Nozomi Nishiumi Masashi Tanimoto Yukiko Kimura Shin-ichi Higashijima |
| author_facet | Sayaka Shimizu Taisei Katayama Nozomi Nishiumi Masashi Tanimoto Yukiko Kimura Shin-ichi Higashijima |
| author_sort | Sayaka Shimizu |
| collection | DOAJ |
| description | Abstract In vertebrates, skeletal muscle comprises fast and slow fibers. Slow and fast muscle cells in fish are spatially segregated; slow muscle cells are located only in a superficial region, and comprise a small fraction of the total muscle cell mass. Slow muscles support low-speed, low-force movements, while fast muscles are responsible for high-speed, high-force movements. However, speed and strength of movement are not binary states, but rather fall on a continuum. This raises the question of whether any recruitment patterns exist within fast muscles, which constitute the majority of muscle cell mass. In the present study, we investigated activation patterns of trunk fast muscles during movements of varying speeds and strengths using larval zebrafish. We employed two complementary methods: calcium imaging and electrophysiology. The results obtained from both methods supported the conclusion that there are spatially-ordered recruitment patterns in fast muscle cells. During weaker/slower movements, only the lateral portion of fast muscle cells is recruited. As the speed or strength of the movements increases, more fast muscle cells are recruited in a spatially-ordered manner, progressively from lateral to medial. We also conducted anatomical studies to examine muscle fiber size. The results of those experiments indicated that muscle fiber size increases systematically from lateral to medial. Therefore, the spatially ordered recruitment of fast muscle fibers, progressing from lateral to medial, correlates with an increase in fiber size. These findings provide significant insights into the organization and function of fast muscles in larval zebrafish, illustrating how spatial recruitment and fiber size interact to optimize movement performance. |
| format | Article |
| id | doaj-art-a90f07df87cd427bb7300ac85b1821e0 |
| institution | Kabale University |
| issn | 2056-306X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Zoological Letters |
| spelling | doaj-art-a90f07df87cd427bb7300ac85b1821e02025-01-05T12:49:44ZengBMCZoological Letters2056-306X2025-01-0111111510.1186/s40851-024-00247-8Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafishSayaka Shimizu0Taisei Katayama1Nozomi Nishiumi2Masashi Tanimoto3Yukiko Kimura4Shin-ichi Higashijima5National Institutes of Natural Sciences, Exploratory Research Center On Life and Living Systems (ExCELLS), National Institute for Basic BiologyNational Institutes of Natural Sciences, Exploratory Research Center On Life and Living Systems (ExCELLS), National Institute for Basic BiologyNational Institutes of Natural Sciences, Exploratory Research Center On Life and Living Systems (ExCELLS), National Institute for Basic BiologyNational Institutes of Natural Sciences, Exploratory Research Center On Life and Living Systems (ExCELLS), National Institute for Basic BiologyNational Institutes of Natural Sciences, Exploratory Research Center On Life and Living Systems (ExCELLS), National Institute for Basic BiologyNational Institutes of Natural Sciences, Exploratory Research Center On Life and Living Systems (ExCELLS), National Institute for Basic BiologyAbstract In vertebrates, skeletal muscle comprises fast and slow fibers. Slow and fast muscle cells in fish are spatially segregated; slow muscle cells are located only in a superficial region, and comprise a small fraction of the total muscle cell mass. Slow muscles support low-speed, low-force movements, while fast muscles are responsible for high-speed, high-force movements. However, speed and strength of movement are not binary states, but rather fall on a continuum. This raises the question of whether any recruitment patterns exist within fast muscles, which constitute the majority of muscle cell mass. In the present study, we investigated activation patterns of trunk fast muscles during movements of varying speeds and strengths using larval zebrafish. We employed two complementary methods: calcium imaging and electrophysiology. The results obtained from both methods supported the conclusion that there are spatially-ordered recruitment patterns in fast muscle cells. During weaker/slower movements, only the lateral portion of fast muscle cells is recruited. As the speed or strength of the movements increases, more fast muscle cells are recruited in a spatially-ordered manner, progressively from lateral to medial. We also conducted anatomical studies to examine muscle fiber size. The results of those experiments indicated that muscle fiber size increases systematically from lateral to medial. Therefore, the spatially ordered recruitment of fast muscle fibers, progressing from lateral to medial, correlates with an increase in fiber size. These findings provide significant insights into the organization and function of fast muscles in larval zebrafish, illustrating how spatial recruitment and fiber size interact to optimize movement performance.https://doi.org/10.1186/s40851-024-00247-8ZebrafishMuscleMovementRecruitmentSwimming |
| spellingShingle | Sayaka Shimizu Taisei Katayama Nozomi Nishiumi Masashi Tanimoto Yukiko Kimura Shin-ichi Higashijima Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish Zoological Letters Zebrafish Muscle Movement Recruitment Swimming |
| title | Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish |
| title_full | Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish |
| title_fullStr | Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish |
| title_full_unstemmed | Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish |
| title_short | Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish |
| title_sort | spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish |
| topic | Zebrafish Muscle Movement Recruitment Swimming |
| url | https://doi.org/10.1186/s40851-024-00247-8 |
| work_keys_str_mv | AT sayakashimizu spatiallyorderedrecruitmentoffastmusclesinaccordancewithmovementstrengthsinlarvalzebrafish AT taiseikatayama spatiallyorderedrecruitmentoffastmusclesinaccordancewithmovementstrengthsinlarvalzebrafish AT nozominishiumi spatiallyorderedrecruitmentoffastmusclesinaccordancewithmovementstrengthsinlarvalzebrafish AT masashitanimoto spatiallyorderedrecruitmentoffastmusclesinaccordancewithmovementstrengthsinlarvalzebrafish AT yukikokimura spatiallyorderedrecruitmentoffastmusclesinaccordancewithmovementstrengthsinlarvalzebrafish AT shinichihigashijima spatiallyorderedrecruitmentoffastmusclesinaccordancewithmovementstrengthsinlarvalzebrafish |