Vitamin D3 supplementation alleviates lipid accumulation, lipid metabolism disorder, and oxidative stress caused by a high-fat diet in juvenile black seabream (Acanthopagrus schlegelii)
This study investigates the potential of dietary vitamin D3 (VD3) to counteract the adverse physiological effects of a high-fat diet (HFD) in marine fish. Five isonitrogenous diets were formulated: a control diet (∼ 12 % lipid, Control), a HFD (∼ 18 % lipid, HFD), and HFDs supplemented with increasi...
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Elsevier
2024-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006100 |
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| author | Hao Cheng Wenli Zhao Yizhou Jiang Yangguang Bao Jiayun Zhu Xinyan Zhi Tingting Zhu Peng Sun Qicun Zhou Min Jin |
| author_facet | Hao Cheng Wenli Zhao Yizhou Jiang Yangguang Bao Jiayun Zhu Xinyan Zhi Tingting Zhu Peng Sun Qicun Zhou Min Jin |
| author_sort | Hao Cheng |
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| description | This study investigates the potential of dietary vitamin D3 (VD3) to counteract the adverse physiological effects of a high-fat diet (HFD) in marine fish. Five isonitrogenous diets were formulated: a control diet (∼ 12 % lipid, Control), a HFD (∼ 18 % lipid, HFD), and HFDs supplemented with increasing levels of VD3 (6000, 12,000, and 18,000 IU/kg), referred to as HFD+6000VD3, HFD+12000VD3 and HFD+18000VD3, respectively. The actual VD3 contents in each diet were 10,400, 10,500, 18,100, 26,300 and 33,700 IU/kg, respectively. A total of 450 juvenile black seabreams (Acanthopagrus schlegelii) with an initial body weight of 5.39 ± 0.01 g were used in an 8-week feeding trial. Results showed that VD3 supplementation significantly up-regulated the expression level of cyp2r1, down-regulated the expression level of cyp24a1, and dramatically increased the expression level of vdrb, leading to higher 1,25(OH)2D3 levels in serum and liver (P < 0.05). Compared to the HFD, fish fed diets supplemented with VD3 exhibited reduced TG and TC levels in serum and liver, decreased expression levels of lipogenesis-related genes (aco3, fas, scd1, srebp-1c) and significantly enhanced the expression levels of lipolysis-related genes (hsl, pparα, lpl, atgl) (P < 0.05). Additionally, dietary VD3 supplementation significantly reduced serum AST and ALT activities (P < 0.05), and decreased the size and number of hepatic lipid droplets and vacuoles (P < 0.05). Furthermore, dietary VD3 inclusion decreased oxidative stress biomarkers, enhanced antioxidant enzyme transcription via the Nrf2 pathway, up-regulated antioxidant genes (cat, nrf2β, Cu-Zn sod), and reduced serum and liver MDA content, alleviating oxidative stress. In conclusion, dietary VD3 supplementation effectively mitigated HFD-induced hepatic fat deposition and lipid metabolism disorders in black seabream. |
| format | Article |
| id | doaj-art-a9074b3276fe4e5b9e7aa845affcc84f |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-a9074b3276fe4e5b9e7aa845affcc84f2024-12-03T04:29:35ZengElsevierAquaculture Reports2352-51342024-12-0139102522Vitamin D3 supplementation alleviates lipid accumulation, lipid metabolism disorder, and oxidative stress caused by a high-fat diet in juvenile black seabream (Acanthopagrus schlegelii)Hao Cheng0Wenli Zhao1Yizhou Jiang2Yangguang Bao3Jiayun Zhu4Xinyan Zhi5Tingting Zhu6Peng Sun7Qicun Zhou8Min Jin9Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, ChinaLaboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, China; Corresponding author at: Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China.This study investigates the potential of dietary vitamin D3 (VD3) to counteract the adverse physiological effects of a high-fat diet (HFD) in marine fish. Five isonitrogenous diets were formulated: a control diet (∼ 12 % lipid, Control), a HFD (∼ 18 % lipid, HFD), and HFDs supplemented with increasing levels of VD3 (6000, 12,000, and 18,000 IU/kg), referred to as HFD+6000VD3, HFD+12000VD3 and HFD+18000VD3, respectively. The actual VD3 contents in each diet were 10,400, 10,500, 18,100, 26,300 and 33,700 IU/kg, respectively. A total of 450 juvenile black seabreams (Acanthopagrus schlegelii) with an initial body weight of 5.39 ± 0.01 g were used in an 8-week feeding trial. Results showed that VD3 supplementation significantly up-regulated the expression level of cyp2r1, down-regulated the expression level of cyp24a1, and dramatically increased the expression level of vdrb, leading to higher 1,25(OH)2D3 levels in serum and liver (P < 0.05). Compared to the HFD, fish fed diets supplemented with VD3 exhibited reduced TG and TC levels in serum and liver, decreased expression levels of lipogenesis-related genes (aco3, fas, scd1, srebp-1c) and significantly enhanced the expression levels of lipolysis-related genes (hsl, pparα, lpl, atgl) (P < 0.05). Additionally, dietary VD3 supplementation significantly reduced serum AST and ALT activities (P < 0.05), and decreased the size and number of hepatic lipid droplets and vacuoles (P < 0.05). Furthermore, dietary VD3 inclusion decreased oxidative stress biomarkers, enhanced antioxidant enzyme transcription via the Nrf2 pathway, up-regulated antioxidant genes (cat, nrf2β, Cu-Zn sod), and reduced serum and liver MDA content, alleviating oxidative stress. In conclusion, dietary VD3 supplementation effectively mitigated HFD-induced hepatic fat deposition and lipid metabolism disorders in black seabream.http://www.sciencedirect.com/science/article/pii/S2352513424006100Acanthopagrus schlegeliiVitamin D3Lipid accumulationampk/ nrf2 signaling pathway |
| spellingShingle | Hao Cheng Wenli Zhao Yizhou Jiang Yangguang Bao Jiayun Zhu Xinyan Zhi Tingting Zhu Peng Sun Qicun Zhou Min Jin Vitamin D3 supplementation alleviates lipid accumulation, lipid metabolism disorder, and oxidative stress caused by a high-fat diet in juvenile black seabream (Acanthopagrus schlegelii) Aquaculture Reports Acanthopagrus schlegelii Vitamin D3 Lipid accumulation ampk/ nrf2 signaling pathway |
| title | Vitamin D3 supplementation alleviates lipid accumulation, lipid metabolism disorder, and oxidative stress caused by a high-fat diet in juvenile black seabream (Acanthopagrus schlegelii) |
| title_full | Vitamin D3 supplementation alleviates lipid accumulation, lipid metabolism disorder, and oxidative stress caused by a high-fat diet in juvenile black seabream (Acanthopagrus schlegelii) |
| title_fullStr | Vitamin D3 supplementation alleviates lipid accumulation, lipid metabolism disorder, and oxidative stress caused by a high-fat diet in juvenile black seabream (Acanthopagrus schlegelii) |
| title_full_unstemmed | Vitamin D3 supplementation alleviates lipid accumulation, lipid metabolism disorder, and oxidative stress caused by a high-fat diet in juvenile black seabream (Acanthopagrus schlegelii) |
| title_short | Vitamin D3 supplementation alleviates lipid accumulation, lipid metabolism disorder, and oxidative stress caused by a high-fat diet in juvenile black seabream (Acanthopagrus schlegelii) |
| title_sort | vitamin d3 supplementation alleviates lipid accumulation lipid metabolism disorder and oxidative stress caused by a high fat diet in juvenile black seabream acanthopagrus schlegelii |
| topic | Acanthopagrus schlegelii Vitamin D3 Lipid accumulation ampk/ nrf2 signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006100 |
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