Metabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination
Abstract Effective Microorganism (EM) is widely employed as a growth promoter in agricultural practices. The aging of oat seeds not only directly impairs agricultural production but also exerts adverse effects on biodiversity. The mechanism through which EM influence the germination of aging seeds r...
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BMC
2025-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-06035-y |
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| author | Yi Hua Linling Dong Shengnan Sun Kexin Wang Yilin Zou Yongqi Gao Ting Gong Guofu Hu Ligang Qin |
| author_facet | Yi Hua Linling Dong Shengnan Sun Kexin Wang Yilin Zou Yongqi Gao Ting Gong Guofu Hu Ligang Qin |
| author_sort | Yi Hua |
| collection | DOAJ |
| description | Abstract Effective Microorganism (EM) is widely employed as a growth promoter in agricultural practices. The aging of oat seeds not only directly impairs agricultural production but also exerts adverse effects on biodiversity. The mechanism through which EM influence the germination of aging seeds remains unclear. In this experiment, the EM bacterial solution underwent pretreatment, which included the original-solution treatment (OrT), supernatant treatment (SuT), and sterile treatment (StT). Aging of oat seeds was induced using the pretreated EM bacterial solution. In this study, the EM bacterial solution facilitated the enhancement of the germination rate, germination index, and vitality index of aged seeds, with SuT demonstrating the most pronounced effects. Specifically, SuT resulted in a significant increase in APX and POD activities, while significantly reducing the malondialdehyde content. In addition, metabolic profiling highlighted the significance of 5-phosphoribosylamine in the purine metabolic pathway. Particularly in the SuT, the upregulation of 5-phosphoribosylamine facilitated the synthesis of (R)-Allantoin, consequently augmenting antioxidant enzyme activity. |
| format | Article |
| id | doaj-art-a8e02ab80c8c46adbc31d95346e7b0f9 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-a8e02ab80c8c46adbc31d95346e7b0f92025-01-12T12:14:22ZengBMCBMC Plant Biology1471-22292025-01-0125111210.1186/s12870-024-06035-yMetabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germinationYi Hua0Linling Dong1Shengnan Sun2Kexin Wang3Yilin Zou4Yongqi Gao5Ting Gong6Guofu Hu7Ligang Qin8Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Northeast Agricultural UniversityAbstract Effective Microorganism (EM) is widely employed as a growth promoter in agricultural practices. The aging of oat seeds not only directly impairs agricultural production but also exerts adverse effects on biodiversity. The mechanism through which EM influence the germination of aging seeds remains unclear. In this experiment, the EM bacterial solution underwent pretreatment, which included the original-solution treatment (OrT), supernatant treatment (SuT), and sterile treatment (StT). Aging of oat seeds was induced using the pretreated EM bacterial solution. In this study, the EM bacterial solution facilitated the enhancement of the germination rate, germination index, and vitality index of aged seeds, with SuT demonstrating the most pronounced effects. Specifically, SuT resulted in a significant increase in APX and POD activities, while significantly reducing the malondialdehyde content. In addition, metabolic profiling highlighted the significance of 5-phosphoribosylamine in the purine metabolic pathway. Particularly in the SuT, the upregulation of 5-phosphoribosylamine facilitated the synthesis of (R)-Allantoin, consequently augmenting antioxidant enzyme activity.https://doi.org/10.1186/s12870-024-06035-yEffective microorganismsAge seedsSeed germinationMetabolomicsAntioxidant enzymes5-Phosphoribosylamine |
| spellingShingle | Yi Hua Linling Dong Shengnan Sun Kexin Wang Yilin Zou Yongqi Gao Ting Gong Guofu Hu Ligang Qin Metabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination BMC Plant Biology Effective microorganisms Age seeds Seed germination Metabolomics Antioxidant enzymes 5-Phosphoribosylamine |
| title | Metabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination |
| title_full | Metabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination |
| title_fullStr | Metabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination |
| title_full_unstemmed | Metabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination |
| title_short | Metabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination |
| title_sort | metabonomics and physiology revealed the critical function of 5 phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination |
| topic | Effective microorganisms Age seeds Seed germination Metabolomics Antioxidant enzymes 5-Phosphoribosylamine |
| url | https://doi.org/10.1186/s12870-024-06035-y |
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