Characteristics of the extended-spectrum-β-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in Taiwan
Abstract A total of 297 Escherichia coli isolates from diseased livestock with diarrhea and 269 isolates from poultry affected by colibacillosis were assessed for extended-spectrum-β-lactamase (ESBL) production. In livestock, 36 isolates (12.1%) were ESBL-producing, with pigs (52.8%), cattle (30.5%)...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-80943-9 |
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| author | Nan-Ling Kuan Yen-Ping Chen Jui-Hung Shien Kuang-Sheng Yeh |
| author_facet | Nan-Ling Kuan Yen-Ping Chen Jui-Hung Shien Kuang-Sheng Yeh |
| author_sort | Nan-Ling Kuan |
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| description | Abstract A total of 297 Escherichia coli isolates from diseased livestock with diarrhea and 269 isolates from poultry affected by colibacillosis were assessed for extended-spectrum-β-lactamase (ESBL) production. In livestock, 36 isolates (12.1%) were ESBL-producing, with pigs (52.8%), cattle (30.5%), and goats (16.7%) being the most affected. Poultry exhibited 22 ESBL-producing strains (8.6%), with distribution among species: chicken (36.3%), duck (22.7%), goose (22.7%), and others (18.2%). ESBL-producing E. coli demonstrated higher drug resistance, except for amoxicillin/clavulanic acid, while all isolates were susceptible to imipenem. The bla CTX−M−55 gene, from the bla CTX−M−1 group, was prevalent in the ESBL-producing E. coli from livestock and poultry. Multilocus sequence typing (MLST) identified distinct sequence types (STs) for 58 ESBL-producing E. coli, except for ST162 and ST1196, detected in both sources. Livestock yielded one ST10 and two ST38 isolates, while poultry exhibited two ST69 and one ST617 isolates, recognized as common extraintestinal pathogenic E. coli (ExPEC) types. In conjugation assays, all ESBL-producing E. coli successfully transferred bla genes to the recipient E. coli J53 strain. The findings underscore food-producing animals as significant ESBL reservoirs, emphasizing the crucial role of judicious antimicrobial use on farms. |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-a8c2daf36fd64c768e529631c278df5f2024-12-01T12:27:16ZengNature PortfolioScientific Reports2045-23222024-11-011411810.1038/s41598-024-80943-9Characteristics of the extended-spectrum-β-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in TaiwanNan-Ling Kuan0Yen-Ping Chen1Jui-Hung Shien2Kuang-Sheng Yeh3Biology Division, Veterinary Research Institute, Ministry of AgricultureBiology Division, Veterinary Research Institute, Ministry of AgricultureThe Animal Disease Diagnostic Center (ADDC), College of Veterinary Medicine, National Chung Hsing UniversityDepartment of Veterinary Medicine, School of Veterinary Medicine, National Taiwan UniversityAbstract A total of 297 Escherichia coli isolates from diseased livestock with diarrhea and 269 isolates from poultry affected by colibacillosis were assessed for extended-spectrum-β-lactamase (ESBL) production. In livestock, 36 isolates (12.1%) were ESBL-producing, with pigs (52.8%), cattle (30.5%), and goats (16.7%) being the most affected. Poultry exhibited 22 ESBL-producing strains (8.6%), with distribution among species: chicken (36.3%), duck (22.7%), goose (22.7%), and others (18.2%). ESBL-producing E. coli demonstrated higher drug resistance, except for amoxicillin/clavulanic acid, while all isolates were susceptible to imipenem. The bla CTX−M−55 gene, from the bla CTX−M−1 group, was prevalent in the ESBL-producing E. coli from livestock and poultry. Multilocus sequence typing (MLST) identified distinct sequence types (STs) for 58 ESBL-producing E. coli, except for ST162 and ST1196, detected in both sources. Livestock yielded one ST10 and two ST38 isolates, while poultry exhibited two ST69 and one ST617 isolates, recognized as common extraintestinal pathogenic E. coli (ExPEC) types. In conjugation assays, all ESBL-producing E. coli successfully transferred bla genes to the recipient E. coli J53 strain. The findings underscore food-producing animals as significant ESBL reservoirs, emphasizing the crucial role of judicious antimicrobial use on farms.https://doi.org/10.1038/s41598-024-80943-9 |
| spellingShingle | Nan-Ling Kuan Yen-Ping Chen Jui-Hung Shien Kuang-Sheng Yeh Characteristics of the extended-spectrum-β-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in Taiwan Scientific Reports |
| title | Characteristics of the extended-spectrum-β-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in Taiwan |
| title_full | Characteristics of the extended-spectrum-β-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in Taiwan |
| title_fullStr | Characteristics of the extended-spectrum-β-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in Taiwan |
| title_full_unstemmed | Characteristics of the extended-spectrum-β-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in Taiwan |
| title_short | Characteristics of the extended-spectrum-β-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in Taiwan |
| title_sort | characteristics of the extended spectrum β lactamase producing escherichia coli isolated from diseased livestock and poultry in taiwan |
| url | https://doi.org/10.1038/s41598-024-80943-9 |
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