Oral microbial community composition is associated with pancreatic cancer: A case‐control study in Iran
Abstract Background Oral microbiota may be related to pancreatic cancer risk because periodontal disease, a condition linked to multiple specific microbes, has been associated with increased risk of pancreatic cancer. We evaluated the association between oral microbiota and pancreatic cancer in Iran...
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| Language: | English |
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Wiley
2020-01-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.2660 |
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| author | Emily Vogtmann Yongli Han J. Gregory Caporaso Nicholas Bokulich Ashraf Mohamadkhani Alireza Moayyedkazemi Xing Hua Farin Kamangar Yunhu Wan Shalabh Suman Bin Zhu Amy Hutchinson Casey Dagnall Kristine Jones Belynda Hicks Jianxin Shi Reza Malekzadeh Christian C. Abnet Akram Pourshams |
| author_facet | Emily Vogtmann Yongli Han J. Gregory Caporaso Nicholas Bokulich Ashraf Mohamadkhani Alireza Moayyedkazemi Xing Hua Farin Kamangar Yunhu Wan Shalabh Suman Bin Zhu Amy Hutchinson Casey Dagnall Kristine Jones Belynda Hicks Jianxin Shi Reza Malekzadeh Christian C. Abnet Akram Pourshams |
| author_sort | Emily Vogtmann |
| collection | DOAJ |
| description | Abstract Background Oral microbiota may be related to pancreatic cancer risk because periodontal disease, a condition linked to multiple specific microbes, has been associated with increased risk of pancreatic cancer. We evaluated the association between oral microbiota and pancreatic cancer in Iran. Methods A total of 273 pancreatic adenocarcinoma cases and 285 controls recruited from tertiary hospitals and a specialty clinic in Tehran, Iran provided saliva samples and filled out a questionnaire regarding demographics and lifestyle characteristics. DNA was extracted from saliva and the V4 region of the 16S rRNA gene was PCR amplified and sequenced on the MiSeq. The sequencing data were processed using the DADA2 plugin in QIIME 2 and taxonomy was assigned against the Human Oral Microbiome Database. Logistic regression and MiRKAT models were calculated with adjustment for potential confounders. Results No association was observed for alpha diversity with an average of 91.11 (standard deviation [SD] 2.59) sequence variants for cases and 89.42 (SD 2.58) for controls. However, there was evidence for an association between beta diversity and case status. The association between the Bray‐Curtis dissimilarity and pancreatic cancer was particularly strong with a MiRKAT P‐value of .000142 and specific principal coordinate vectors had strong associations with cancer risk. Several specific taxa were also associated with case status after adjustment for multiple comparisons. Conclusion The overall microbial community appeared to differ between pancreatic cancer cases and controls. Whether these reflect differences evident before development of pancreatic cancer will need to be evaluated in prospective studies. |
| format | Article |
| id | doaj-art-a8c03f6f606e4e6fa363a9a6015b0e88 |
| institution | Kabale University |
| issn | 2045-7634 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-a8c03f6f606e4e6fa363a9a6015b0e882025-08-25T10:14:05ZengWileyCancer Medicine2045-76342020-01-019279780610.1002/cam4.2660Oral microbial community composition is associated with pancreatic cancer: A case‐control study in IranEmily Vogtmann0Yongli Han1J. Gregory Caporaso2Nicholas Bokulich3Ashraf Mohamadkhani4Alireza Moayyedkazemi5Xing Hua6Farin Kamangar7Yunhu Wan8Shalabh Suman9Bin Zhu10Amy Hutchinson11Casey Dagnall12Kristine Jones13Belynda Hicks14Jianxin Shi15Reza Malekzadeh16Christian C. Abnet17Akram Pourshams18Metabolic Epidemiology Branch Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USABiostatistics Branch Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USACenter for Applied Microbiome Science Pathogen and Microbiome Institute Northern Arizona University Flagstaff AZ USACenter for Applied Microbiome Science Pathogen and Microbiome Institute Northern Arizona University Flagstaff AZ USADigestive Oncology Research Center Digestive Diseases Research Institute Tehran University of Medical Sciences Tehran IranDepartment of Internal Medicine Lorestan University of Medical Sciences Khorramabad IranBiostatistics Branch Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USADepartment of Biology School of Computer, Mathematical, and Natural Sciences Morgan State University Baltimore MD USABiostatistics Branch Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USACancer Genomics Research Laboratory Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USACancer Genomics Research Laboratory Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USACancer Genomics Research Laboratory Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USACancer Genomics Research Laboratory Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USACancer Genomics Research Laboratory Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USACancer Genomics Research Laboratory Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USABiostatistics Branch Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USADigestive Oncology Research Center Digestive Diseases Research Institute Tehran University of Medical Sciences Tehran IranMetabolic Epidemiology Branch Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USADigestive Oncology Research Center Digestive Diseases Research Institute Tehran University of Medical Sciences Tehran IranAbstract Background Oral microbiota may be related to pancreatic cancer risk because periodontal disease, a condition linked to multiple specific microbes, has been associated with increased risk of pancreatic cancer. We evaluated the association between oral microbiota and pancreatic cancer in Iran. Methods A total of 273 pancreatic adenocarcinoma cases and 285 controls recruited from tertiary hospitals and a specialty clinic in Tehran, Iran provided saliva samples and filled out a questionnaire regarding demographics and lifestyle characteristics. DNA was extracted from saliva and the V4 region of the 16S rRNA gene was PCR amplified and sequenced on the MiSeq. The sequencing data were processed using the DADA2 plugin in QIIME 2 and taxonomy was assigned against the Human Oral Microbiome Database. Logistic regression and MiRKAT models were calculated with adjustment for potential confounders. Results No association was observed for alpha diversity with an average of 91.11 (standard deviation [SD] 2.59) sequence variants for cases and 89.42 (SD 2.58) for controls. However, there was evidence for an association between beta diversity and case status. The association between the Bray‐Curtis dissimilarity and pancreatic cancer was particularly strong with a MiRKAT P‐value of .000142 and specific principal coordinate vectors had strong associations with cancer risk. Several specific taxa were also associated with case status after adjustment for multiple comparisons. Conclusion The overall microbial community appeared to differ between pancreatic cancer cases and controls. Whether these reflect differences evident before development of pancreatic cancer will need to be evaluated in prospective studies.https://doi.org/10.1002/cam4.2660case‐control studymicrobiotapancreatic cancer |
| spellingShingle | Emily Vogtmann Yongli Han J. Gregory Caporaso Nicholas Bokulich Ashraf Mohamadkhani Alireza Moayyedkazemi Xing Hua Farin Kamangar Yunhu Wan Shalabh Suman Bin Zhu Amy Hutchinson Casey Dagnall Kristine Jones Belynda Hicks Jianxin Shi Reza Malekzadeh Christian C. Abnet Akram Pourshams Oral microbial community composition is associated with pancreatic cancer: A case‐control study in Iran Cancer Medicine case‐control study microbiota pancreatic cancer |
| title | Oral microbial community composition is associated with pancreatic cancer: A case‐control study in Iran |
| title_full | Oral microbial community composition is associated with pancreatic cancer: A case‐control study in Iran |
| title_fullStr | Oral microbial community composition is associated with pancreatic cancer: A case‐control study in Iran |
| title_full_unstemmed | Oral microbial community composition is associated with pancreatic cancer: A case‐control study in Iran |
| title_short | Oral microbial community composition is associated with pancreatic cancer: A case‐control study in Iran |
| title_sort | oral microbial community composition is associated with pancreatic cancer a case control study in iran |
| topic | case‐control study microbiota pancreatic cancer |
| url | https://doi.org/10.1002/cam4.2660 |
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