RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma
Abstract Background Our aim was to identify crucial RNA-binding proteins (RBP) genes associated with Ewing sarcoma (EwS) in order to provide valuable insights into its mechanisms of tumorigenesis and to enhance therapeutic intervention. Results Differential gene expression analysis identified candid...
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BMC
2025-05-01
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| Series: | Hereditas |
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| Online Access: | https://doi.org/10.1186/s41065-025-00440-5 |
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| author | Yannan Geng Lu Yang Rui Shao Tiantong Xu Lilong Zhang |
| author_facet | Yannan Geng Lu Yang Rui Shao Tiantong Xu Lilong Zhang |
| author_sort | Yannan Geng |
| collection | DOAJ |
| description | Abstract Background Our aim was to identify crucial RNA-binding proteins (RBP) genes associated with Ewing sarcoma (EwS) in order to provide valuable insights into its mechanisms of tumorigenesis and to enhance therapeutic intervention. Results Differential gene expression analysis identified candidate genes. Next, hub genes were generated by the results of protein-protein interaction (PPI) network, and univariate COX regression analysis. CIBERSORT was applied to analyze immune landscape. Furthermore, both in vitro and in vivo experiments were conducted to investigate the function of NOP58 in EwS. Results A total of 179 RBP-related genes were significantly different in EwS tissues and normal controls. Among these, NOP58 ribonucleoprotein (NOP58) was considered as the hub gene, demonstrating significant prognostic value. Significantly, high NOP58 expression correlated with poor prognosis of EwS patients. Additionally, the levels of NOP58 were significantly up-regulated in EwS cells compared with human mesenchymal stem cells. Furthermore, knockdown of NOP58 notably inhibited the proliferation and migration of EwS cells. Moreover, NOP58 deficiency remarkably induced apoptosis and cell cycle arrest in EwS cells. In vivo studies on tumor-bearing mice demonstrated that NOP58 downregulation significantly inhibited tumor growth in EwS. Conclusion Collectively, downregulation of NOP58 could inhibit the proliferation and migration of EwS cells in vitro and reduce murine xenograft tumor growth in vivo. These findings identified NOP58 as a promising regulator of EwS tumorigenesis, suggesting it may serve as a potential therapeutic target for EwS treatment. |
| format | Article |
| id | doaj-art-a8b963c7545d4a51b5c26bb87f067fa7 |
| institution | Kabale University |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | Hereditas |
| spelling | doaj-art-a8b963c7545d4a51b5c26bb87f067fa72025-08-20T03:48:06ZengBMCHereditas1601-52232025-05-01162111810.1186/s41065-025-00440-5RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcomaYannan Geng0Lu Yang1Rui Shao2Tiantong Xu3Lilong Zhang4Department of the Sixth Spinal Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai UniversityThe Third Department of Breast Cancer, Tianjin Medical University Cancer Institute and HospitalDepartment of the Sixth Spinal Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai UniversityDepartment of the Sixth Spinal Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai UniversityDepartment of the Sixth Spinal Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai UniversityAbstract Background Our aim was to identify crucial RNA-binding proteins (RBP) genes associated with Ewing sarcoma (EwS) in order to provide valuable insights into its mechanisms of tumorigenesis and to enhance therapeutic intervention. Results Differential gene expression analysis identified candidate genes. Next, hub genes were generated by the results of protein-protein interaction (PPI) network, and univariate COX regression analysis. CIBERSORT was applied to analyze immune landscape. Furthermore, both in vitro and in vivo experiments were conducted to investigate the function of NOP58 in EwS. Results A total of 179 RBP-related genes were significantly different in EwS tissues and normal controls. Among these, NOP58 ribonucleoprotein (NOP58) was considered as the hub gene, demonstrating significant prognostic value. Significantly, high NOP58 expression correlated with poor prognosis of EwS patients. Additionally, the levels of NOP58 were significantly up-regulated in EwS cells compared with human mesenchymal stem cells. Furthermore, knockdown of NOP58 notably inhibited the proliferation and migration of EwS cells. Moreover, NOP58 deficiency remarkably induced apoptosis and cell cycle arrest in EwS cells. In vivo studies on tumor-bearing mice demonstrated that NOP58 downregulation significantly inhibited tumor growth in EwS. Conclusion Collectively, downregulation of NOP58 could inhibit the proliferation and migration of EwS cells in vitro and reduce murine xenograft tumor growth in vivo. These findings identified NOP58 as a promising regulator of EwS tumorigenesis, suggesting it may serve as a potential therapeutic target for EwS treatment.https://doi.org/10.1186/s41065-025-00440-5NOP58Ewing sarcomaRNA-binding proteinPrognosisCell growth |
| spellingShingle | Yannan Geng Lu Yang Rui Shao Tiantong Xu Lilong Zhang RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma Hereditas NOP58 Ewing sarcoma RNA-binding protein Prognosis Cell growth |
| title | RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma |
| title_full | RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma |
| title_fullStr | RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma |
| title_full_unstemmed | RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma |
| title_short | RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma |
| title_sort | rna binding protein gene nop58 exhibits crucial prognostic and therapeutic value in ewing sarcoma |
| topic | NOP58 Ewing sarcoma RNA-binding protein Prognosis Cell growth |
| url | https://doi.org/10.1186/s41065-025-00440-5 |
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