ZAM-CS, a novel chimeric endolysin with enhanced stability and rapid action against methicillin-resistant Staphylococcus aureus
Abstract Background The rising occurrence of antibiotic resistance in Staphylococcus aureus significantly complicates its treatment. Endolysins are now recognized as a promising substitute for antibiotics in combating multidrug-resistant bacteria. In this research, a novel chimeric endolysin named Z...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-06-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-025-04074-5 |
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| Summary: | Abstract Background The rising occurrence of antibiotic resistance in Staphylococcus aureus significantly complicates its treatment. Endolysins are now recognized as a promising substitute for antibiotics in combating multidrug-resistant bacteria. In this research, a novel chimeric endolysin named ZAM-CS was engineered and assessed to achieve enhanced stability, solubility, and rapid bactericidal activity. This was accomplished by combining the catalytic domain of the SAL-1 endolysin with the peptidoglycan binding domain of lysostaphin. Results Expression and purification outcomes indicated that ZAM-CS exhibited significant solubility, with a yield of approximately 23 mg/L. ZAM-CS demonstrated high stability under various temperature conditions and retained most of its activity in both acidic and alkaline pH. The results of turbidity reduction assay showed a 50% decrease in the initial OD600 within 10 min at a minimum concentration of 2 µg/mL. The minimum inhibitory concentration and the minimum bactericidal concentration values of ZAM-CS are identical in the methicillin-resistant Staphylococcus aureus strain, indicating the strong bactericidal properties of this endolysin. Antimicrobial tests showed ZAM-CS was also effective against coagulase-negative Staphylococci, Streptococcus agalactiae, and Enterococcus faecalis. Conclusion The rapid and dynamic action and high stability of ZAM-CS compared to other recombinant endolysins make it a suitable candidate for development to replace antibiotics targeting harmful Staphylococci, including MRSA. |
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| ISSN: | 1471-2180 |