Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS Accumulation
(1) T-2 toxin synthesized by <i>Fusarium oxysporum</i> (<i>F. oxysporum</i>) can cause deterioration of dried fish and endanger human health. (2) The molecular mechanism by which antibacterial lipopeptides surfactin inhibited <i>F. oxysporum</i> growth and toxin p...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/29/22/5402 |
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| Summary: | (1) T-2 toxin synthesized by <i>Fusarium oxysporum</i> (<i>F. oxysporum</i>) can cause deterioration of dried fish and endanger human health. (2) The molecular mechanism by which antibacterial lipopeptides surfactin inhibited <i>F. oxysporum</i> growth and toxin production was elucidated by investigating the intracellular ROS production pathway and the subcellular distribution and transcriptional activity of the transcription factor <i>Yap1</i> and its regulation of <i>Tri5</i> gene in <i>F. oxysporum</i>. (3) Surfactin caused hyphal damage and nucleic acid and protein leakage; thus, the growth of <i>F. oxysporum</i> was disrupted. Surfactin’s excessive accumulation of intracellular ROS triggered the translocation of transcription factor <i>Yap1</i> into the nucleus, resulting in toxin cluster gene <i>Tri5</i> expression inhibition, thereby blocking T-2 toxin synthesis. (4) This is a novel mechanism by which surfactin inhibits the growth and T-2 toxin synthesis of <i>F. oxysporum</i> from multiple aspects, including cell structural integrity and the ROS-<i>Yap1</i> signaling pathway. (5) This study provides a theoretical basis for the application of surfactin in the antifungal control of aquatic dry products. |
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| ISSN: | 1420-3049 |