Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp.
Botryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mu...
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Universitas Negeri Semarang
2018-08-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/14891 |
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| author | Windra Yuniarsih Heli Siti Halimatul Asri Peni Wulandari |
| author_facet | Windra Yuniarsih Heli Siti Halimatul Asri Peni Wulandari |
| author_sort | Windra Yuniarsih |
| collection | DOAJ |
| description | Botryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mutagenic engineering using irradiation has been known to increase the content of these biomass. The aim of this study is to know the effect of electron-beam irradiation to the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after being exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strain B160, B240, and B320, respectively. The profile growth was significantly different between control (0 kGy) and irradiated microalgae strain. The highest growth is found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp. |
| format | Article |
| id | doaj-art-a859de3f48e946568b70cc23b2ce6a98 |
| institution | Kabale University |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-a859de3f48e946568b70cc23b2ce6a982025-08-20T03:38:54ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102018-08-0110238839410.15294/biosaintifika.v10i2.148917530Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp.Windra Yuniarsih0Heli Siti Halimatul1Asri Peni Wulandari2Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas PadjadjaranDepartment of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Pendidikan IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas PadjadjaranBotryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mutagenic engineering using irradiation has been known to increase the content of these biomass. The aim of this study is to know the effect of electron-beam irradiation to the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after being exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strain B160, B240, and B320, respectively. The profile growth was significantly different between control (0 kGy) and irradiated microalgae strain. The highest growth is found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/14891botryococcus sp.electron-beamfatty acids |
| spellingShingle | Windra Yuniarsih Heli Siti Halimatul Asri Peni Wulandari Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. Biosaintifika: Journal of Biology & Biology Education botryococcus sp. electron-beam fatty acids |
| title | Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. |
| title_full | Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. |
| title_fullStr | Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. |
| title_full_unstemmed | Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. |
| title_short | Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. |
| title_sort | effect of electron beam irradiation on the growth profile and fatty acid composition of botryococcus sp |
| topic | botryococcus sp. electron-beam fatty acids |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/14891 |
| work_keys_str_mv | AT windrayuniarsih effectofelectronbeamirradiationonthegrowthprofileandfattyacidcompositionofbotryococcussp AT helisitihalimatul effectofelectronbeamirradiationonthegrowthprofileandfattyacidcompositionofbotryococcussp AT asripeniwulandari effectofelectronbeamirradiationonthegrowthprofileandfattyacidcompositionofbotryococcussp |