Antagonistic effects of endophytic fungi from Camellia reticulata pedicels on yeasts: implications for antimicrobial mechanism of nectar
Endophytic fungi are extensive in plant tissues and involved in the defense against stress from harmful microbes. The interaction between pedicel endophytic fungi and nectar yeasts is critical for maintaining nectar homeostasis. This study used Camellia reticulata as the research subject. High-throu...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1494855/full |
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| author | Rong Huang Qingxin Meng Lijie Xun Lijie Xun Xiaoman Wu Dan Yue Wenzheng Zhao Xia Dong Xueyang Gong Kun Dong |
| author_facet | Rong Huang Qingxin Meng Lijie Xun Lijie Xun Xiaoman Wu Dan Yue Wenzheng Zhao Xia Dong Xueyang Gong Kun Dong |
| author_sort | Rong Huang |
| collection | DOAJ |
| description | Endophytic fungi are extensive in plant tissues and involved in the defense against stress from harmful microbes. The interaction between pedicel endophytic fungi and nectar yeasts is critical for maintaining nectar homeostasis. This study used Camellia reticulata as the research subject. High-throughput sequencing revealed that the community composition of endophytic fungi in the pedicel is dominated by Ascomycota and Basidiomycota. Their abundance varies at different taxonomic levels, showing sample variability. In total, 27 endophytic fungal isolates were isolated and screened from the pedicel under laboratory conditions. They exhibited antagonistic effects against three nectar yeasts (Metschnikowia reukaufii, Cryptococcus laurentii, and Rhodotorula glutinis) and displayed morphological and physiological diversity. The isolates were classified into the phylum Ascomycota and further categorized into the genera Alternaria, Trichoderma, Fusarium, and Dactylaria. The endophytic fungus D23, which effectively antagonizes nectar yeasts, was identified as Alternaria alternata. This fungus produces various secondary metabolites, including antibiotics such as penicillin G, grandiomycin, and cephalosporin C. The metabolic pathways involved include the biosynthesis of plant secondary metabolites, phenylpropanoids, amino acids, nucleotides, and antibiotics. The endophytic fungal community in C. reticulata pedicel is rich and diverse, making it a valuable material for screening antagonistic strains. This study provides a theoretical basis for the antagonistic effects of endophytic fungal metabolites from the pedicel of C. reticulata against nectar yeasts, highlighting their significance in maintaining nectar stability and reproductive fitness in cross-pollinated plants. |
| format | Article |
| id | doaj-art-a821d27efe1d4c499e4a34eb42c1c00c |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-a821d27efe1d4c499e4a34eb42c1c00c2024-11-15T04:52:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14948551494855Antagonistic effects of endophytic fungi from Camellia reticulata pedicels on yeasts: implications for antimicrobial mechanism of nectarRong Huang0Qingxin Meng1Lijie Xun2Lijie Xun3Xiaoman Wu4Dan Yue5Wenzheng Zhao6Xia Dong7Xueyang Gong8Kun Dong9Yunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaInstitute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, ChinaYunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Engineering and Research Center for Sustainable Utilization of Honey Bee Resources, Eastern Bee Research Institute, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, ChinaEndophytic fungi are extensive in plant tissues and involved in the defense against stress from harmful microbes. The interaction between pedicel endophytic fungi and nectar yeasts is critical for maintaining nectar homeostasis. This study used Camellia reticulata as the research subject. High-throughput sequencing revealed that the community composition of endophytic fungi in the pedicel is dominated by Ascomycota and Basidiomycota. Their abundance varies at different taxonomic levels, showing sample variability. In total, 27 endophytic fungal isolates were isolated and screened from the pedicel under laboratory conditions. They exhibited antagonistic effects against three nectar yeasts (Metschnikowia reukaufii, Cryptococcus laurentii, and Rhodotorula glutinis) and displayed morphological and physiological diversity. The isolates were classified into the phylum Ascomycota and further categorized into the genera Alternaria, Trichoderma, Fusarium, and Dactylaria. The endophytic fungus D23, which effectively antagonizes nectar yeasts, was identified as Alternaria alternata. This fungus produces various secondary metabolites, including antibiotics such as penicillin G, grandiomycin, and cephalosporin C. The metabolic pathways involved include the biosynthesis of plant secondary metabolites, phenylpropanoids, amino acids, nucleotides, and antibiotics. The endophytic fungal community in C. reticulata pedicel is rich and diverse, making it a valuable material for screening antagonistic strains. This study provides a theoretical basis for the antagonistic effects of endophytic fungal metabolites from the pedicel of C. reticulata against nectar yeasts, highlighting their significance in maintaining nectar stability and reproductive fitness in cross-pollinated plants.https://www.frontiersin.org/articles/10.3389/fpls.2024.1494855/fullCamellia reticulataendophytic funginectar yeastsantagonistic activityantimicrobial mechanismAlternaria alternata |
| spellingShingle | Rong Huang Qingxin Meng Lijie Xun Lijie Xun Xiaoman Wu Dan Yue Wenzheng Zhao Xia Dong Xueyang Gong Kun Dong Antagonistic effects of endophytic fungi from Camellia reticulata pedicels on yeasts: implications for antimicrobial mechanism of nectar Frontiers in Plant Science Camellia reticulata endophytic fungi nectar yeasts antagonistic activity antimicrobial mechanism Alternaria alternata |
| title | Antagonistic effects of endophytic fungi from Camellia reticulata pedicels on yeasts: implications for antimicrobial mechanism of nectar |
| title_full | Antagonistic effects of endophytic fungi from Camellia reticulata pedicels on yeasts: implications for antimicrobial mechanism of nectar |
| title_fullStr | Antagonistic effects of endophytic fungi from Camellia reticulata pedicels on yeasts: implications for antimicrobial mechanism of nectar |
| title_full_unstemmed | Antagonistic effects of endophytic fungi from Camellia reticulata pedicels on yeasts: implications for antimicrobial mechanism of nectar |
| title_short | Antagonistic effects of endophytic fungi from Camellia reticulata pedicels on yeasts: implications for antimicrobial mechanism of nectar |
| title_sort | antagonistic effects of endophytic fungi from camellia reticulata pedicels on yeasts implications for antimicrobial mechanism of nectar |
| topic | Camellia reticulata endophytic fungi nectar yeasts antagonistic activity antimicrobial mechanism Alternaria alternata |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1494855/full |
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