Expression of toll-like receptors (tlrs) in the equine peripheral blood mononuclear cells (pbmcs) during the early pregnancy and estrous cycle
<b>Aim:</b> Practical, reliable and economical pregnancy diagnosis method for as early as possible time of pregnancy in mares is still requirement. Immune tolerance against allogenic fetus is critically important during the early pregnancy. Tolllike Receptors (TLRs) are known to be an im...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=459 |
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| Summary: | <b>Aim:</b> Practical, reliable and economical pregnancy diagnosis
method for as early as possible time of pregnancy in mares
is still requirement. Immune tolerance against allogenic fetus
is critically important during the early pregnancy. Tolllike
Receptors (TLRs) are known to be an important part of
innate immune system. Aim was to evaluate expression of
TLRs in peripheral blood mononuclear cells (PBMCs) during
the early pregnancy in mare.<p>
<b>Material and Methods:</b> Blood samples were collected on
days of ovulation (d0) and 8 during estrous and pregnancy
from three same mares. Total RNA samples were isolated
from PBMCs and cDNA synthesis was performed. Expressions
of TLRs at mRNA levels were quantified by using realtime
RT-PCR in duplicates. Relative Expression Software
Tool (REST2009) was used for statistical analysis.<p>
<b>Results:</b> TLRs (1 to 10) were found to be expressed in PMBCs
by RT-PCR. However, real-time PCR measurements
failed to show effects of pregnant or cyclic status on expression
of TLRs.<p>
<b>Conclusion:</b> According to these results, it may suggest that
determination of TLRs gene expression in equine PBMCs
during early pregnancy is not a good marker in understanding
early equine pregnancy. |
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| ISSN: | 1309-6958 2146-1953 |