Preliminary Functional Analysis of the Gut Microbiome in Colic Horses
The gut microbiome plays a critical role in maintaining horse health, influencing digestion, immunity, and overall well-being. However, in certain conditions like colic, there is evidence of significant alterations in the microbial community. To analyze the composition of the fecal microbiome and th...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/22/3222 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846154712786665472 |
|---|---|
| author | Pamela Thomson Daniel Garrido Rodrigo Santibáñez Felipe Lara |
| author_facet | Pamela Thomson Daniel Garrido Rodrigo Santibáñez Felipe Lara |
| author_sort | Pamela Thomson |
| collection | DOAJ |
| description | The gut microbiome plays a critical role in maintaining horse health, influencing digestion, immunity, and overall well-being. However, in certain conditions like colic, there is evidence of significant alterations in the microbial community. To analyze the composition of the fecal microbiome and the enriched predicted metabolic functions of horses with colic compared to a control group, 14 horses with colic and 14 control horses were recruited. From a stool sample, DNA extraction was carried out for subsequent 16S rRNA metagenomic analysis. The composition of the microbiome was analyzed from the sequences of each sample using the QIIME version 1.8.0 and DADA2 version 1.22 programs. PICRUSt2 was used to predict metabolic functions. Statistical analyses were performed with the Mann–Whitney U test from the Python scipy v1 package. The gut microbiomes of both groups were dominated by <i>Firmicuteota</i>, <i>Bacteroidota</i>, and <i>Pseudomonadota</i> phyla. Colic in horses was associated with reduced diversity, reduced abundance of <i>Fibrobacter</i>, and an increase in <i>Streptococcus</i>. The abundance of <i>Firmicuteota</i> was negatively correlated with <i>Pseudomonadota</i> and <i>Actinobacteriota.</i> The equine colic microbiome was predicted to be enriched in aerobic respiration pathways and fatty acid and amino acid degradation. These observations indicate discrete but important differences in the gut microbiome of colic horses. |
| format | Article |
| id | doaj-art-a7ce87fc9a1a4d4c86c9de182ad63a79 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-a7ce87fc9a1a4d4c86c9de182ad63a792024-11-26T17:45:47ZengMDPI AGAnimals2076-26152024-11-011422322210.3390/ani14223222Preliminary Functional Analysis of the Gut Microbiome in Colic HorsesPamela Thomson0Daniel Garrido1Rodrigo Santibáñez2Felipe Lara3Laboratorio de Microbiología Clínica y Microbioma, Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 7550196, ChileDepartamento de Ingeniería Química y Bioprocesos, Facultad de Ingeniería, Pontificia Universidad Católica de Chile, Santiago 8331150, ChileDepartamento de Ingeniería Química y Bioprocesos, Facultad de Ingeniería, Pontificia Universidad Católica de Chile, Santiago 8331150, ChileUnidad de Medicina y Cirugía Equina, Hospital Clínico Veterinario, Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 7550196, ChileThe gut microbiome plays a critical role in maintaining horse health, influencing digestion, immunity, and overall well-being. However, in certain conditions like colic, there is evidence of significant alterations in the microbial community. To analyze the composition of the fecal microbiome and the enriched predicted metabolic functions of horses with colic compared to a control group, 14 horses with colic and 14 control horses were recruited. From a stool sample, DNA extraction was carried out for subsequent 16S rRNA metagenomic analysis. The composition of the microbiome was analyzed from the sequences of each sample using the QIIME version 1.8.0 and DADA2 version 1.22 programs. PICRUSt2 was used to predict metabolic functions. Statistical analyses were performed with the Mann–Whitney U test from the Python scipy v1 package. The gut microbiomes of both groups were dominated by <i>Firmicuteota</i>, <i>Bacteroidota</i>, and <i>Pseudomonadota</i> phyla. Colic in horses was associated with reduced diversity, reduced abundance of <i>Fibrobacter</i>, and an increase in <i>Streptococcus</i>. The abundance of <i>Firmicuteota</i> was negatively correlated with <i>Pseudomonadota</i> and <i>Actinobacteriota.</i> The equine colic microbiome was predicted to be enriched in aerobic respiration pathways and fatty acid and amino acid degradation. These observations indicate discrete but important differences in the gut microbiome of colic horses.https://www.mdpi.com/2076-2615/14/22/3222gut microbiomeequinecolic |
| spellingShingle | Pamela Thomson Daniel Garrido Rodrigo Santibáñez Felipe Lara Preliminary Functional Analysis of the Gut Microbiome in Colic Horses Animals gut microbiome equine colic |
| title | Preliminary Functional Analysis of the Gut Microbiome in Colic Horses |
| title_full | Preliminary Functional Analysis of the Gut Microbiome in Colic Horses |
| title_fullStr | Preliminary Functional Analysis of the Gut Microbiome in Colic Horses |
| title_full_unstemmed | Preliminary Functional Analysis of the Gut Microbiome in Colic Horses |
| title_short | Preliminary Functional Analysis of the Gut Microbiome in Colic Horses |
| title_sort | preliminary functional analysis of the gut microbiome in colic horses |
| topic | gut microbiome equine colic |
| url | https://www.mdpi.com/2076-2615/14/22/3222 |
| work_keys_str_mv | AT pamelathomson preliminaryfunctionalanalysisofthegutmicrobiomeincolichorses AT danielgarrido preliminaryfunctionalanalysisofthegutmicrobiomeincolichorses AT rodrigosantibanez preliminaryfunctionalanalysisofthegutmicrobiomeincolichorses AT felipelara preliminaryfunctionalanalysisofthegutmicrobiomeincolichorses |