Derivation of two induced pluripotent stem cell lines from a healthy control subject
Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124003192 |
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| author | Dan Zhang Di Huang Leon M. Larcher Khine Zaw Shang-Chih Chen Luke Jennings Tina M. Lamey Jennifer A. Thompson Terri L. McLaren Fred K. Chen Samuel McLenachan |
| author_facet | Dan Zhang Di Huang Leon M. Larcher Khine Zaw Shang-Chih Chen Luke Jennings Tina M. Lamey Jennifer A. Thompson Terri L. McLaren Fred K. Chen Samuel McLenachan |
| author_sort | Dan Zhang |
| collection | DOAJ |
| description | Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells. |
| format | Article |
| id | doaj-art-a798642d53644d09b7d90e8383d0c111 |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-a798642d53644d09b7d90e8383d0c1112025-01-13T04:18:38ZengElsevierStem Cell Research1873-50612025-02-0182103621Derivation of two induced pluripotent stem cell lines from a healthy control subjectDan Zhang0Di Huang1Leon M. Larcher2Khine Zaw3Shang-Chih Chen4Luke Jennings5Tina M. Lamey6Jennifer A. Thompson7Terri L. McLaren8Fred K. Chen9Samuel McLenachan10Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, AustraliaOcular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, AustraliaOcular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, AustraliaOcular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, AustraliaOcular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, AustraliaOcular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, AustraliaAustralian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaAustralian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaOcular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia; Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, Australia; Department of Ophthalmology, Royal Perth Hospital, Perth, Western Australia, Australia; Department of Ophthalmology, Perth Children’s Hospital, Nedlands, Western Australia, Australia; Corresponding authors.Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia; Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Corresponding authors.Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.http://www.sciencedirect.com/science/article/pii/S1873506124003192 |
| spellingShingle | Dan Zhang Di Huang Leon M. Larcher Khine Zaw Shang-Chih Chen Luke Jennings Tina M. Lamey Jennifer A. Thompson Terri L. McLaren Fred K. Chen Samuel McLenachan Derivation of two induced pluripotent stem cell lines from a healthy control subject Stem Cell Research |
| title | Derivation of two induced pluripotent stem cell lines from a healthy control subject |
| title_full | Derivation of two induced pluripotent stem cell lines from a healthy control subject |
| title_fullStr | Derivation of two induced pluripotent stem cell lines from a healthy control subject |
| title_full_unstemmed | Derivation of two induced pluripotent stem cell lines from a healthy control subject |
| title_short | Derivation of two induced pluripotent stem cell lines from a healthy control subject |
| title_sort | derivation of two induced pluripotent stem cell lines from a healthy control subject |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124003192 |
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