Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimen
Introduction: Nonthermal atmospheric pressure plasma (NTAPP) therapy has emerged as a promising modality in dermatology for wound healing, tissue regeneration, and anti-tumor applications. This study evaluated the feasibility and effectiveness of a simple, one-week NTAPP treatment protocol in the SK...
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Elsevier
2025-02-01
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| Series: | Journal of Photochemistry and Photobiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666469024000332 |
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| author | Szabolcs Bozsányi Ruby Acquah Rhea Carmel Glen Rodrigues Erin C. Tracy Sean P. Murphy Gregory Fridman Wendy J. Huss Peter C. Friedman Gyorgy Paragh |
| author_facet | Szabolcs Bozsányi Ruby Acquah Rhea Carmel Glen Rodrigues Erin C. Tracy Sean P. Murphy Gregory Fridman Wendy J. Huss Peter C. Friedman Gyorgy Paragh |
| author_sort | Szabolcs Bozsányi |
| collection | DOAJ |
| description | Introduction: Nonthermal atmospheric pressure plasma (NTAPP) therapy has emerged as a promising modality in dermatology for wound healing, tissue regeneration, and anti-tumor applications. This study evaluated the feasibility and effectiveness of a simple, one-week NTAPP treatment protocol in the SKH1 hairless mouse model of chronic ultraviolet (UV) radiation-induced carcinogenesis. Materials and methods: SKH1 mice were exposed to solar-simulated UV light 5 times a week for 10 weeks, which produced multiple skin tumors on each mouse. At week 25 NTAPP treatment was administered to a subgroup of mice at a setting of 20 kV with a 20 ns pulse width and 200 Hz frequency three times within a single week. A total of 31 NTAPP-treated and 34 internal control tumors (i.e., nearby tumors on the same mouse) in UV-exposed, NTAPP-treated mice were evaluated for tumor size at 0 and 28 days after the beginning of the treatment by using ImageJ on standardized photographs. A subset of the tumors was also assessed with a 33 MHz high-frequency ultrasound (HFUS) to measure vertical tumor thickness. In similarly housed control mice, sizes of 64 tumors were evaluated as external controls. Results: NTAPP induced a significant reduction in the mean tumor area from 5.65 ± 6.51 mm² before treatment to 1.74 ± 3.99 mm² 28 days post-treatment (paired t-test, p = 0.0016). Internal control tumors, in contrast, showed an increase in area from 3.24 ± 2.54 mm² before treatment to 4.57 ± 3.58 mm² 28 days post-treatment (paired t-test, p = 0.0296). Of the 31 treated tumors, 23 completely disappeared; in contrast, only six non-treated internal control tumors disappeared (p = 0.0139, two-sided chi-square test). Simultaneously, optically guided HFUS revealed a significant decrease in tumor thickness in treated areas (paired t-test, p = 0.0006), with no significant changes observed in the internal control tumors (paired t-test, p = 0.5555). External control tumors showed an increase in size, which was not statistically significant. Discussion: The results demonstrated that a one-week NTAPP protocol could effectively eliminate 74 % of mouse tumors induced by UV radiation. These findings warrant further investigations of short, clinically feasible NTAPP treatment protocols for early skin cancer treatment. |
| format | Article |
| id | doaj-art-a7719d152b904ad3850b0379bc07d506 |
| institution | Kabale University |
| issn | 2666-4690 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Photochemistry and Photobiology |
| spelling | doaj-art-a7719d152b904ad3850b0379bc07d5062025-01-12T05:25:50ZengElsevierJournal of Photochemistry and Photobiology2666-46902025-02-0125100258Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimenSzabolcs Bozsányi0Ruby Acquah1Rhea Carmel Glen Rodrigues2Erin C. Tracy3Sean P. Murphy4Gregory Fridman5Wendy J. Huss6Peter C. Friedman7Gyorgy Paragh8Department of Dermatology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USADepartment of Dermatology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USADepartment of Dermatology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USADepartment of Dermatology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USADepartment of Dermatology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USAAAPlasma LLC, Philadelphia, PA, 19146, USADepartment of Dermatology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USAThe Skin Center Dermatology Group, New City, NY, 10956, USA; Corresponding authors.Department of Dermatology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA; Corresponding authors.Introduction: Nonthermal atmospheric pressure plasma (NTAPP) therapy has emerged as a promising modality in dermatology for wound healing, tissue regeneration, and anti-tumor applications. This study evaluated the feasibility and effectiveness of a simple, one-week NTAPP treatment protocol in the SKH1 hairless mouse model of chronic ultraviolet (UV) radiation-induced carcinogenesis. Materials and methods: SKH1 mice were exposed to solar-simulated UV light 5 times a week for 10 weeks, which produced multiple skin tumors on each mouse. At week 25 NTAPP treatment was administered to a subgroup of mice at a setting of 20 kV with a 20 ns pulse width and 200 Hz frequency three times within a single week. A total of 31 NTAPP-treated and 34 internal control tumors (i.e., nearby tumors on the same mouse) in UV-exposed, NTAPP-treated mice were evaluated for tumor size at 0 and 28 days after the beginning of the treatment by using ImageJ on standardized photographs. A subset of the tumors was also assessed with a 33 MHz high-frequency ultrasound (HFUS) to measure vertical tumor thickness. In similarly housed control mice, sizes of 64 tumors were evaluated as external controls. Results: NTAPP induced a significant reduction in the mean tumor area from 5.65 ± 6.51 mm² before treatment to 1.74 ± 3.99 mm² 28 days post-treatment (paired t-test, p = 0.0016). Internal control tumors, in contrast, showed an increase in area from 3.24 ± 2.54 mm² before treatment to 4.57 ± 3.58 mm² 28 days post-treatment (paired t-test, p = 0.0296). Of the 31 treated tumors, 23 completely disappeared; in contrast, only six non-treated internal control tumors disappeared (p = 0.0139, two-sided chi-square test). Simultaneously, optically guided HFUS revealed a significant decrease in tumor thickness in treated areas (paired t-test, p = 0.0006), with no significant changes observed in the internal control tumors (paired t-test, p = 0.5555). External control tumors showed an increase in size, which was not statistically significant. Discussion: The results demonstrated that a one-week NTAPP protocol could effectively eliminate 74 % of mouse tumors induced by UV radiation. These findings warrant further investigations of short, clinically feasible NTAPP treatment protocols for early skin cancer treatment.http://www.sciencedirect.com/science/article/pii/S2666469024000332Nonthermal atmospheric pressure plasmaNTAPPUVSkin cancerEarly topical treatment |
| spellingShingle | Szabolcs Bozsányi Ruby Acquah Rhea Carmel Glen Rodrigues Erin C. Tracy Sean P. Murphy Gregory Fridman Wendy J. Huss Peter C. Friedman Gyorgy Paragh Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimen Journal of Photochemistry and Photobiology Nonthermal atmospheric pressure plasma NTAPP UV Skin cancer Early topical treatment |
| title | Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimen |
| title_full | Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimen |
| title_fullStr | Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimen |
| title_full_unstemmed | Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimen |
| title_short | Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimen |
| title_sort | nonthermal atmospheric pressure plasma ntapp effectively eliminates skin tumors in a mouse model of uv induced carcinogenesis with a short term treatment regimen |
| topic | Nonthermal atmospheric pressure plasma NTAPP UV Skin cancer Early topical treatment |
| url | http://www.sciencedirect.com/science/article/pii/S2666469024000332 |
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