Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.)

Abstract The sea toad genus Chaunax is a group of small benthic fishes that predominantly inhabiting the deep seas of the Atlantic, Indian, and Pacific Oceans. Although they have the potential to make excellent systems for studies of evolutionary adaptation to deep-sea environments, genomic research...

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Main Authors: Zifeng Zhan, Yanshuo Liang, Jiehong Wei, Jing Liu, Kuidong Xu
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-024-04245-5
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author Zifeng Zhan
Yanshuo Liang
Jiehong Wei
Jing Liu
Kuidong Xu
author_facet Zifeng Zhan
Yanshuo Liang
Jiehong Wei
Jing Liu
Kuidong Xu
author_sort Zifeng Zhan
collection DOAJ
description Abstract The sea toad genus Chaunax is a group of small benthic fishes that predominantly inhabiting the deep seas of the Atlantic, Indian, and Pacific Oceans. Although they have the potential to make excellent systems for studies of evolutionary adaptation to deep-sea environments, genomic research on Chaunax has been hindered by a scarcity of high-quality genomic resources. We present a chromosome-scale genome assembly of a Chaunax specimen generated using PacBio long-read sequencing and high-throughput chromosome conformation capture technology. The size of the assembled genome was 706.94 Mb, with a contig N50 of 15.24 Mb and scaffold N50 of 29.42 Mb. Approximately 96.11% of assembled sequences were anchored and oriented onto 24 pseudo-chromosomes. The genome contained 213.47 Mb repetitive sequences, 25,280 protein-coding genes, and 5,090 non-coding RNAs. The high ratio of complete BUSCO genes (97.20%) indicates high quality of genome assembly. The chromosomal-level reference genome of Chaunax sp. provides a preliminary molecular basis for understanding deep-sea adaptation and phenotypic evolution as well as an important reference for whole-genome sequencing of related species.
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institution Kabale University
issn 2052-4463
language English
publishDate 2024-12-01
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spelling doaj-art-a71c88259fa34fbdb9a7dcf93a53c48d2024-12-22T12:13:48ZengNature PortfolioScientific Data2052-44632024-12-011111910.1038/s41597-024-04245-5Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.)Zifeng Zhan0Yanshuo Liang1Jiehong Wei2Jing Liu3Kuidong Xu4Laboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Institute of Oceanology, Chinese Academy of SciencesLaboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Institute of Oceanology, Chinese Academy of SciencesLaboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Institute of Oceanology, Chinese Academy of SciencesLaboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Institute of Oceanology, Chinese Academy of SciencesLaboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Institute of Oceanology, Chinese Academy of SciencesAbstract The sea toad genus Chaunax is a group of small benthic fishes that predominantly inhabiting the deep seas of the Atlantic, Indian, and Pacific Oceans. Although they have the potential to make excellent systems for studies of evolutionary adaptation to deep-sea environments, genomic research on Chaunax has been hindered by a scarcity of high-quality genomic resources. We present a chromosome-scale genome assembly of a Chaunax specimen generated using PacBio long-read sequencing and high-throughput chromosome conformation capture technology. The size of the assembled genome was 706.94 Mb, with a contig N50 of 15.24 Mb and scaffold N50 of 29.42 Mb. Approximately 96.11% of assembled sequences were anchored and oriented onto 24 pseudo-chromosomes. The genome contained 213.47 Mb repetitive sequences, 25,280 protein-coding genes, and 5,090 non-coding RNAs. The high ratio of complete BUSCO genes (97.20%) indicates high quality of genome assembly. The chromosomal-level reference genome of Chaunax sp. provides a preliminary molecular basis for understanding deep-sea adaptation and phenotypic evolution as well as an important reference for whole-genome sequencing of related species.https://doi.org/10.1038/s41597-024-04245-5
spellingShingle Zifeng Zhan
Yanshuo Liang
Jiehong Wei
Jing Liu
Kuidong Xu
Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.)
Scientific Data
title Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.)
title_full Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.)
title_fullStr Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.)
title_full_unstemmed Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.)
title_short Chromosome-level genome assembly and annotation of a sea toad (Chaunax sp.)
title_sort chromosome level genome assembly and annotation of a sea toad chaunax sp
url https://doi.org/10.1038/s41597-024-04245-5
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AT jiehongwei chromosomelevelgenomeassemblyandannotationofaseatoadchaunaxsp
AT jingliu chromosomelevelgenomeassemblyandannotationofaseatoadchaunaxsp
AT kuidongxu chromosomelevelgenomeassemblyandannotationofaseatoadchaunaxsp