Monitoring the Olfactory Evolution of Cold-Fermented Sourdough Using an Electronic Nose

The quality of artisanal bread is strongly influenced by sourdough fermentation, where aroma development and microbial stability are key factors. This study evaluates the use of an electronic nose (E-nose) to monitor cold fermentation, integrating it with microbiological analysis and gas chromatogra...

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Main Authors: Elisabetta Poeta, Estefanía Núñez-Carmona, Veronica Sberveglieri, Jesús Lozano, Ramiro Sánchez
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Chemosensors
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Online Access:https://www.mdpi.com/2227-9040/13/5/187
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author Elisabetta Poeta
Estefanía Núñez-Carmona
Veronica Sberveglieri
Jesús Lozano
Ramiro Sánchez
author_facet Elisabetta Poeta
Estefanía Núñez-Carmona
Veronica Sberveglieri
Jesús Lozano
Ramiro Sánchez
author_sort Elisabetta Poeta
collection DOAJ
description The quality of artisanal bread is strongly influenced by sourdough fermentation, where aroma development and microbial stability are key factors. This study evaluates the use of an electronic nose (E-nose) to monitor cold fermentation, integrating it with microbiological analysis and gas chromatography–mass spectrometry (SPME-GC-MS) to characterize the dough’s volatile profile. A clear correlation was observed between microbial dynamics, pH reduction (from 5.8 to 3.8), and the evolution of volatile compounds, with notable increases in acetic acid (up to 12.75%), ethanol (11.95%), and fruity esters such as isoamyl acetate (33.33%). Linear discriminant analysis (LDA) explained 96.31% of the total variance in a single component, successfully separating the fermentation stages. An artificial neural network discriminant analysis (ANNDA) model achieved 95% accuracy in the validation phase. These results confirm the E-nose’s ability to track biochemical transformations in real time and identify optimal fermentation points. This approach enhances quality control and sensory standardization in sourdough-based bakery products.
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institution Kabale University
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publishDate 2025-05-01
publisher MDPI AG
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series Chemosensors
spelling doaj-art-a71b652fbec44b67b70a44abb1b66e692025-08-20T03:47:49ZengMDPI AGChemosensors2227-90402025-05-0113518710.3390/chemosensors13050187Monitoring the Olfactory Evolution of Cold-Fermented Sourdough Using an Electronic NoseElisabetta Poeta0Estefanía Núñez-Carmona1Veronica Sberveglieri2Jesús Lozano3Ramiro Sánchez4Department of Life Sciences, University of Modena and Reggio Emilia, Via J.F. Kennedy, 17/i, 42124 Reggio Emilia, RE, ItalyInstitute of Bioscience and Bioresources (CNR-IBBR), National Research Council URT Reggio Emilia, Via J.F. Kennedy, 17/i, 42124 Reggio Emilia, RE, ItalyInstitute of Bioscience and Bioresources (CNR-IBBR), National Research Council URT Reggio Emilia, Via J.F. Kennedy, 17/i, 42124 Reggio Emilia, RE, ItalyIndustrial Engineering School, University of Extremadura, 06006 Badajoz, SpainCentro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), 06006 Badajoz, SpainThe quality of artisanal bread is strongly influenced by sourdough fermentation, where aroma development and microbial stability are key factors. This study evaluates the use of an electronic nose (E-nose) to monitor cold fermentation, integrating it with microbiological analysis and gas chromatography–mass spectrometry (SPME-GC-MS) to characterize the dough’s volatile profile. A clear correlation was observed between microbial dynamics, pH reduction (from 5.8 to 3.8), and the evolution of volatile compounds, with notable increases in acetic acid (up to 12.75%), ethanol (11.95%), and fruity esters such as isoamyl acetate (33.33%). Linear discriminant analysis (LDA) explained 96.31% of the total variance in a single component, successfully separating the fermentation stages. An artificial neural network discriminant analysis (ANNDA) model achieved 95% accuracy in the validation phase. These results confirm the E-nose’s ability to track biochemical transformations in real time and identify optimal fermentation points. This approach enhances quality control and sensory standardization in sourdough-based bakery products.https://www.mdpi.com/2227-9040/13/5/187dynamics of fermentationnanotechnologiesquality optimizationvolatile organic compoundssensors
spellingShingle Elisabetta Poeta
Estefanía Núñez-Carmona
Veronica Sberveglieri
Jesús Lozano
Ramiro Sánchez
Monitoring the Olfactory Evolution of Cold-Fermented Sourdough Using an Electronic Nose
Chemosensors
dynamics of fermentation
nanotechnologies
quality optimization
volatile organic compounds
sensors
title Monitoring the Olfactory Evolution of Cold-Fermented Sourdough Using an Electronic Nose
title_full Monitoring the Olfactory Evolution of Cold-Fermented Sourdough Using an Electronic Nose
title_fullStr Monitoring the Olfactory Evolution of Cold-Fermented Sourdough Using an Electronic Nose
title_full_unstemmed Monitoring the Olfactory Evolution of Cold-Fermented Sourdough Using an Electronic Nose
title_short Monitoring the Olfactory Evolution of Cold-Fermented Sourdough Using an Electronic Nose
title_sort monitoring the olfactory evolution of cold fermented sourdough using an electronic nose
topic dynamics of fermentation
nanotechnologies
quality optimization
volatile organic compounds
sensors
url https://www.mdpi.com/2227-9040/13/5/187
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AT estefanianunezcarmona monitoringtheolfactoryevolutionofcoldfermentedsourdoughusinganelectronicnose
AT veronicasberveglieri monitoringtheolfactoryevolutionofcoldfermentedsourdoughusinganelectronicnose
AT jesuslozano monitoringtheolfactoryevolutionofcoldfermentedsourdoughusinganelectronicnose
AT ramirosanchez monitoringtheolfactoryevolutionofcoldfermentedsourdoughusinganelectronicnose