CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles
Abstract The bean beetle (Callosobruchus maculatus) clearly exhibits population density-dependent polymorphism. Cytochrome P450 (CYP) is involved in many aspects of the physiological activities of insects. However, the role of CYP in population density-dependent polymorphisms remains unknown. The te...
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Nature Portfolio
2024-11-01
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| author | Qianquan Chen Yongqin Li Zheng Fang Qingshan Wu Leitao Tan Qingbei Weng |
| author_facet | Qianquan Chen Yongqin Li Zheng Fang Qingshan Wu Leitao Tan Qingbei Weng |
| author_sort | Qianquan Chen |
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| description | Abstract The bean beetle (Callosobruchus maculatus) clearly exhibits population density-dependent polymorphism. Cytochrome P450 (CYP) is involved in many aspects of the physiological activities of insects. However, the role of CYP in population density-dependent polymorphisms remains unknown. The terminal oocyte maturity rate of high-population-density individuals (H) was faster than that of low-population-density individuals (L). A total of 56 CYP-like genes were identified from transcriptomic and genomic data, including seven clan 2 CYP-like genes, seven mitochondrial CYP-like genes, 19 clan 3 CYP-like genes, and 23 clan 4 CYP-like genes. Gene duplication might occur in CYP9Z4-like, CYP345A1-like, CYP345A2-like, CYP349A1-like, CYP349A2-like, and CYP4BN4-like. Thirteen and two CYP-like genes were up-regulated and down-regulated, respectively, in H. Among these CYP-like genes, CYP4BN4v7-like was the most abundant CYP. CYP4BN4v7-like was more highly expressed in the head than in the thorax and abdomen. Its mRNA levels in the head, thorax, and abdomen were greater in H than in L. After RNA interference decreased its mRNA level, the terminal oocyte maturity rate decreased. Moreover, the expression level of insulin-like peptide 1 (ILP1), which plays a vital role in regulating terminal oocyte development, decreased in the head. In conclusion, CYP4BN4v7-like modulated the population density-dependent terminal oocyte maturity rate by regulating the expression of ILP1. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-a6fd4168600e4ff2b80e0b24ed52cb6d2024-11-24T12:20:43ZengNature PortfolioScientific Reports2045-23222024-11-0114111110.1038/s41598-024-79866-2CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetlesQianquan Chen0Yongqin Li1Zheng Fang2Qingshan Wu3Leitao Tan4Qingbei Weng5School of Life Sciences, Guizhou Normal UniversitySchool of Life Sciences, Guizhou Normal UniversitySchool of Life Sciences, Guizhou Normal UniversitySchool of Life Sciences, Guizhou Normal UniversitySchool of Life Sciences, Guizhou Normal UniversitySchool of Life Sciences, Guizhou Normal UniversityAbstract The bean beetle (Callosobruchus maculatus) clearly exhibits population density-dependent polymorphism. Cytochrome P450 (CYP) is involved in many aspects of the physiological activities of insects. However, the role of CYP in population density-dependent polymorphisms remains unknown. The terminal oocyte maturity rate of high-population-density individuals (H) was faster than that of low-population-density individuals (L). A total of 56 CYP-like genes were identified from transcriptomic and genomic data, including seven clan 2 CYP-like genes, seven mitochondrial CYP-like genes, 19 clan 3 CYP-like genes, and 23 clan 4 CYP-like genes. Gene duplication might occur in CYP9Z4-like, CYP345A1-like, CYP345A2-like, CYP349A1-like, CYP349A2-like, and CYP4BN4-like. Thirteen and two CYP-like genes were up-regulated and down-regulated, respectively, in H. Among these CYP-like genes, CYP4BN4v7-like was the most abundant CYP. CYP4BN4v7-like was more highly expressed in the head than in the thorax and abdomen. Its mRNA levels in the head, thorax, and abdomen were greater in H than in L. After RNA interference decreased its mRNA level, the terminal oocyte maturity rate decreased. Moreover, the expression level of insulin-like peptide 1 (ILP1), which plays a vital role in regulating terminal oocyte development, decreased in the head. In conclusion, CYP4BN4v7-like modulated the population density-dependent terminal oocyte maturity rate by regulating the expression of ILP1.https://doi.org/10.1038/s41598-024-79866-2P450RNAiStored-product insectPhenotypic plasticityGene duplication |
| spellingShingle | Qianquan Chen Yongqin Li Zheng Fang Qingshan Wu Leitao Tan Qingbei Weng CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles Scientific Reports P450 RNAi Stored-product insect Phenotypic plasticity Gene duplication |
| title | CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles |
| title_full | CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles |
| title_fullStr | CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles |
| title_full_unstemmed | CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles |
| title_short | CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles |
| title_sort | cyp4bn4v7 regulates the population density dependent oocyte maturity rate in bean beetles |
| topic | P450 RNAi Stored-product insect Phenotypic plasticity Gene duplication |
| url | https://doi.org/10.1038/s41598-024-79866-2 |
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