Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis
Polybutylene succinate (PBS) is a biodegradable polyester with better processability and different mechanical properties compared to polylactides (PLAs), the most commonly used synthetic polymers in tissue engineering (TE). Since only few studies have evaluated PBS-containing materials for bone TE,...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/5928935 |
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| author | Miina Ojansivu Laura Johansson Sari Vanhatupa Ilmari Tamminen Markus Hannula Jari Hyttinen Minna Kellomäki Susanna Miettinen |
| author_facet | Miina Ojansivu Laura Johansson Sari Vanhatupa Ilmari Tamminen Markus Hannula Jari Hyttinen Minna Kellomäki Susanna Miettinen |
| author_sort | Miina Ojansivu |
| collection | DOAJ |
| description | Polybutylene succinate (PBS) is a biodegradable polyester with better processability and different mechanical properties compared to polylactides (PLAs), the most commonly used synthetic polymers in tissue engineering (TE). Since only few studies have evaluated PBS-containing materials for bone TE, we prepared PLA-PBS blends and analyzed material properties as well as cell attachment, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) on scaffolds. In addition to PLA, PBS, and PLA-PBS blends, PLA-polycaprolactone and PLA-poly(trimethylene carbonate) blends were evaluated. Polymer fibers were prepared using melt spinning. Pure PBS was observed to have the highest crystallinity and strain at break compared to the tougher PLA and PLA blends. No degradation occurred during the 4-week hydrolysis in either of the materials. Knitted and rolled scaffolds were manufactured, seeded with hMSCs, and cultured for 27 days. Human MSC viability was good on all the materials, but cell spreading along the fibers was only detected in PBS-containing scaffolds. They also induced the strongest proliferative response and osteogenic differentiation, which diminished with decreasing PBS content. Based on these results, PBS is superior to PLA with respect to hMSC attachment, proliferation, and osteogenesis. This encourages utilizing PBS-based biomaterials more widely in bone TE applications. |
| format | Article |
| id | doaj-art-a6d65d4e0bcc49b59f1cb05e0ef40b10 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a6d65d4e0bcc49b59f1cb05e0ef40b102025-08-20T03:38:39ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/59289355928935Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and OsteogenesisMiina Ojansivu0Laura Johansson1Sari Vanhatupa2Ilmari Tamminen3Markus Hannula4Jari Hyttinen5Minna Kellomäki6Susanna Miettinen7Adult Stem Cell Research Group, Faculty of Medicine and Life Sciences and BioMediTech Institute, University of Tampere, Tampere, FinlandLaboratory of Biomaterials and Tissue Engineering, Faculty of Biomedical Science and Engineering and BioMediTech Institute, Tampere University of Technology, Tampere, FinlandAdult Stem Cell Research Group, Faculty of Medicine and Life Sciences and BioMediTech Institute, University of Tampere, Tampere, FinlandThe Computational Biophysics and Imaging Group, Faculty of Biomedical Science and Engineering and BioMediTech Institute, Tampere University of Technology, Tampere, FinlandThe Computational Biophysics and Imaging Group, Faculty of Biomedical Science and Engineering and BioMediTech Institute, Tampere University of Technology, Tampere, FinlandThe Computational Biophysics and Imaging Group, Faculty of Biomedical Science and Engineering and BioMediTech Institute, Tampere University of Technology, Tampere, FinlandLaboratory of Biomaterials and Tissue Engineering, Faculty of Biomedical Science and Engineering and BioMediTech Institute, Tampere University of Technology, Tampere, FinlandAdult Stem Cell Research Group, Faculty of Medicine and Life Sciences and BioMediTech Institute, University of Tampere, Tampere, FinlandPolybutylene succinate (PBS) is a biodegradable polyester with better processability and different mechanical properties compared to polylactides (PLAs), the most commonly used synthetic polymers in tissue engineering (TE). Since only few studies have evaluated PBS-containing materials for bone TE, we prepared PLA-PBS blends and analyzed material properties as well as cell attachment, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) on scaffolds. In addition to PLA, PBS, and PLA-PBS blends, PLA-polycaprolactone and PLA-poly(trimethylene carbonate) blends were evaluated. Polymer fibers were prepared using melt spinning. Pure PBS was observed to have the highest crystallinity and strain at break compared to the tougher PLA and PLA blends. No degradation occurred during the 4-week hydrolysis in either of the materials. Knitted and rolled scaffolds were manufactured, seeded with hMSCs, and cultured for 27 days. Human MSC viability was good on all the materials, but cell spreading along the fibers was only detected in PBS-containing scaffolds. They also induced the strongest proliferative response and osteogenic differentiation, which diminished with decreasing PBS content. Based on these results, PBS is superior to PLA with respect to hMSC attachment, proliferation, and osteogenesis. This encourages utilizing PBS-based biomaterials more widely in bone TE applications.http://dx.doi.org/10.1155/2018/5928935 |
| spellingShingle | Miina Ojansivu Laura Johansson Sari Vanhatupa Ilmari Tamminen Markus Hannula Jari Hyttinen Minna Kellomäki Susanna Miettinen Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis Stem Cells International |
| title | Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis |
| title_full | Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis |
| title_fullStr | Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis |
| title_full_unstemmed | Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis |
| title_short | Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis |
| title_sort | knitted 3d scaffolds of polybutylene succinate support human mesenchymal stem cell growth and osteogenesis |
| url | http://dx.doi.org/10.1155/2018/5928935 |
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