Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital
Two different limulus amoebocyte lysate assays of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital are evaluated. The gel-clot LAL method and turbidimetric kinetic method were applied as techniques for determination of endotoxin from Gram – negative bac...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University Ss Cyril and Methodius in Skopje, Faculty of Pharmacy and Macedonian Pharmaceutical Association
2024-12-01
|
| Series: | Makedonsko Farmacevtski Bilten |
| Subjects: | |
| Online Access: | https://bulletin.mfd.org.mk/volumes/Volume%2070_1/70_1_007.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841545848321736704 |
|---|---|
| author | Renata Bozhinovska Popovska Tatjana Gjurichikj Branislava Janeva Jelena Acevska Aneta Dimitrovska |
| author_facet | Renata Bozhinovska Popovska Tatjana Gjurichikj Branislava Janeva Jelena Acevska Aneta Dimitrovska |
| author_sort | Renata Bozhinovska Popovska |
| collection | DOAJ |
| description | Two different limulus amoebocyte lysate assays of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical
hospital are evaluated. The gel-clot LAL method and turbidimetric kinetic method were applied as techniques for determination of
endotoxin from Gram – negative bacteria in a large volume of 0.9% NaCl solution for infusion. The same principle is applied but using
different official methods for determination of bacterial endotoxin′s concertation. An overview of the advantages and disadvantages to the
implementation of both methods is provided, with a detailed description of the procedures, comparison of the suitability of each method and recommendations based on the findings. The conventional gel-clot LAL method as a simple method can be recommended due to its
economic sustainability in terms of equipment. On the other hand, the turbidimetric kinetic assay offers distinct advantages such as
enhanced sensitivity, quantitative analysis capability, and instrumental reading. Furthermore, this method helps eliminate potential human
variability in endpoint assessments.
Therefore, the obtained results and critical aspects of the applied methods: the gel-clot LAL method and the turbidimetric kinetic
method indicate that both LAL methods can be used for the determination of bacterial endotoxins in parenteral solutions, produced on a small scale and provide an opportunity for small laboratories to facilitate the decision – making process for choosing a method that best
meets their requirements and resources.
|
| format | Article |
| id | doaj-art-a6cfca07a14a4a78997fad5d37df909d |
| institution | Kabale University |
| issn | 1857-8969 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | University Ss Cyril and Methodius in Skopje, Faculty of Pharmacy and Macedonian Pharmaceutical Association |
| record_format | Article |
| series | Makedonsko Farmacevtski Bilten |
| spelling | doaj-art-a6cfca07a14a4a78997fad5d37df909d2025-01-11T13:40:29ZengUniversity Ss Cyril and Methodius in Skopje, Faculty of Pharmacy and Macedonian Pharmaceutical AssociationMakedonsko Farmacevtski Bilten1857-89692024-12-01701616810.33320/maced.pharm.bull.2024.70.01.007Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospitalRenata Bozhinovska Popovska0Tatjana Gjurichikj1Branislava Janeva2Jelena Acevska3Aneta Dimitrovska4Clinic Hospital “dr. Trifun Panovski“, Partizanska bb, 7000 Bitola, R. North MacedoniaAlkaloid Ad, Blvd Aleksandar Makedonski No 12, 1000 Skopje, R. North MacedoniaPH – Institute for public health, 50 th division No 6, 1000 Skopje,R. North MacedoniaFaculty of Pharmacy, Ss Cyril Methodius University in Skopje, Majka Thereza 47, 1000 Skopje, R. North MacedoniaFaculty of Pharmacy, Ss Cyril Methodius University in Skopje, Majka Thereza 47, 1000 Skopje, R. North MacedoniaTwo different limulus amoebocyte lysate assays of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital are evaluated. The gel-clot LAL method and turbidimetric kinetic method were applied as techniques for determination of endotoxin from Gram – negative bacteria in a large volume of 0.9% NaCl solution for infusion. The same principle is applied but using different official methods for determination of bacterial endotoxin′s concertation. An overview of the advantages and disadvantages to the implementation of both methods is provided, with a detailed description of the procedures, comparison of the suitability of each method and recommendations based on the findings. The conventional gel-clot LAL method as a simple method can be recommended due to its economic sustainability in terms of equipment. On the other hand, the turbidimetric kinetic assay offers distinct advantages such as enhanced sensitivity, quantitative analysis capability, and instrumental reading. Furthermore, this method helps eliminate potential human variability in endpoint assessments. Therefore, the obtained results and critical aspects of the applied methods: the gel-clot LAL method and the turbidimetric kinetic method indicate that both LAL methods can be used for the determination of bacterial endotoxins in parenteral solutions, produced on a small scale and provide an opportunity for small laboratories to facilitate the decision – making process for choosing a method that best meets their requirements and resources. https://bulletin.mfd.org.mk/volumes/Volume%2070_1/70_1_007.pdfdetermination of bacterial endotoxinsgel-clot lal methodturbidimetric kinetic methodsmall scale production |
| spellingShingle | Renata Bozhinovska Popovska Tatjana Gjurichikj Branislava Janeva Jelena Acevska Aneta Dimitrovska Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital Makedonsko Farmacevtski Bilten determination of bacterial endotoxins gel-clot lal method turbidimetric kinetic method small scale production |
| title | Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital |
| title_full | Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital |
| title_fullStr | Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital |
| title_full_unstemmed | Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital |
| title_short | Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital |
| title_sort | determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital |
| topic | determination of bacterial endotoxins gel-clot lal method turbidimetric kinetic method small scale production |
| url | https://bulletin.mfd.org.mk/volumes/Volume%2070_1/70_1_007.pdf |
| work_keys_str_mv | AT renatabozhinovskapopovska determinationofbacterialendotoxinsinparenteralsolutionsproducedonasmallscaleinaclinicalhospital AT tatjanagjurichikj determinationofbacterialendotoxinsinparenteralsolutionsproducedonasmallscaleinaclinicalhospital AT branislavajaneva determinationofbacterialendotoxinsinparenteralsolutionsproducedonasmallscaleinaclinicalhospital AT jelenaacevska determinationofbacterialendotoxinsinparenteralsolutionsproducedonasmallscaleinaclinicalhospital AT anetadimitrovska determinationofbacterialendotoxinsinparenteralsolutionsproducedonasmallscaleinaclinicalhospital |