Determination of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital
Two different limulus amoebocyte lysate assays of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical hospital are evaluated. The gel-clot LAL method and turbidimetric kinetic method were applied as techniques for determination of endotoxin from Gram – negative bac...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University Ss Cyril and Methodius in Skopje, Faculty of Pharmacy and Macedonian Pharmaceutical Association
2024-12-01
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| Series: | Makedonsko Farmacevtski Bilten |
| Subjects: | |
| Online Access: | https://bulletin.mfd.org.mk/volumes/Volume%2070_1/70_1_007.pdf |
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| Summary: | Two different limulus amoebocyte lysate assays of bacterial endotoxins in parenteral solutions produced on a small scale in a clinical
hospital are evaluated. The gel-clot LAL method and turbidimetric kinetic method were applied as techniques for determination of
endotoxin from Gram – negative bacteria in a large volume of 0.9% NaCl solution for infusion. The same principle is applied but using
different official methods for determination of bacterial endotoxin′s concertation. An overview of the advantages and disadvantages to the
implementation of both methods is provided, with a detailed description of the procedures, comparison of the suitability of each method and recommendations based on the findings. The conventional gel-clot LAL method as a simple method can be recommended due to its
economic sustainability in terms of equipment. On the other hand, the turbidimetric kinetic assay offers distinct advantages such as
enhanced sensitivity, quantitative analysis capability, and instrumental reading. Furthermore, this method helps eliminate potential human
variability in endpoint assessments.
Therefore, the obtained results and critical aspects of the applied methods: the gel-clot LAL method and the turbidimetric kinetic
method indicate that both LAL methods can be used for the determination of bacterial endotoxins in parenteral solutions, produced on a small scale and provide an opportunity for small laboratories to facilitate the decision – making process for choosing a method that best
meets their requirements and resources.
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| ISSN: | 1857-8969 |