Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G
Measuring whole-brain distributed functional activity is an important unmet need in neuroscience, requiring high temporal resolution and cellular specificity across large volumes. Functional optoacoustic neuro-tomography (FONT) with genetically encoded calcium ion indicators is a promising approach...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Photoacoustics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597924000880 |
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| author | Sarah F. Shaykevich Justin P. Little Yong Qian Marie-Eve Paquet Robert E. Campbell Daniel Razansky Shy Shoham |
| author_facet | Sarah F. Shaykevich Justin P. Little Yong Qian Marie-Eve Paquet Robert E. Campbell Daniel Razansky Shy Shoham |
| author_sort | Sarah F. Shaykevich |
| collection | DOAJ |
| description | Measuring whole-brain distributed functional activity is an important unmet need in neuroscience, requiring high temporal resolution and cellular specificity across large volumes. Functional optoacoustic neuro-tomography (FONT) with genetically encoded calcium ion indicators is a promising approach towards this goal. However, it has not yet been applied in the near-infrared (NIR) range that provides deep penetration and low vascular background optimal for in vivo neuroimaging. Here, we study the noninvasive multimodal fluorescence and optoacoustic imaging performance of state-of-the-art NIR calcium ion indicator NIR-GECO2G in the mouse brain. We observe robust in vivo signals with widefield fluorescence, and for the first time, with FONT. We also show that in both modalities, the NIR-GECO2G signal improves more than twofold in the biliverdin-enriched Blvra-/- mouse line compared to wild type. Our findings demonstrate the potential of multimodal fluorescence and optoacoustic NIR imaging, opening new possibilities for whole-brain real-time functional imaging in rodents. |
| format | Article |
| id | doaj-art-a63b9ae566ed4e179278b9c9c35717e8 |
| institution | Kabale University |
| issn | 2213-5979 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj-art-a63b9ae566ed4e179278b9c9c35717e82025-01-17T04:49:30ZengElsevierPhotoacoustics2213-59792025-02-0141100671Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2GSarah F. Shaykevich0Justin P. Little1Yong Qian2Marie-Eve Paquet3Robert E. Campbell4Daniel Razansky5Shy Shoham6NYU Langone Health, Tech4Health and Neuroscience Institutes, and Department of Ophthalmology, New York City, USANYU Langone Health, Tech4Health and Neuroscience Institutes, and Department of Ophthalmology, New York City, USAMassachusetts Institute of Technology, McGovern Institute for Brain Research, Cambridge, MA, USA; The University of Tokyo, Department of Chemistry, Tokyo, JapanCERVO Brain Research Centre, Québec, Canada; Université Laval, Department of Biochemistry, Microbiology and Bioinformatics, Québec, CanadaThe University of Tokyo, Department of Chemistry, Tokyo, Japan; CERVO Brain Research Centre, Québec, Canada; Université Laval, Department of Biochemistry, Microbiology and Bioinformatics, Québec, CanadaUniversity of Zurich, Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, Zurich, Switzerland; ETH Zurich, Institute for Biomedical Engineering, Department of Information Technologies and Electrical Engineering, Zurich, SwitzerlandNYU Langone Health, Tech4Health and Neuroscience Institutes, and Department of Ophthalmology, New York City, USA; Correspondence to: Tech4Health and Neuroscience Institutes, and Department of Ophthalmology, NYU Langone Health, NY 10016, USA.Measuring whole-brain distributed functional activity is an important unmet need in neuroscience, requiring high temporal resolution and cellular specificity across large volumes. Functional optoacoustic neuro-tomography (FONT) with genetically encoded calcium ion indicators is a promising approach towards this goal. However, it has not yet been applied in the near-infrared (NIR) range that provides deep penetration and low vascular background optimal for in vivo neuroimaging. Here, we study the noninvasive multimodal fluorescence and optoacoustic imaging performance of state-of-the-art NIR calcium ion indicator NIR-GECO2G in the mouse brain. We observe robust in vivo signals with widefield fluorescence, and for the first time, with FONT. We also show that in both modalities, the NIR-GECO2G signal improves more than twofold in the biliverdin-enriched Blvra-/- mouse line compared to wild type. Our findings demonstrate the potential of multimodal fluorescence and optoacoustic NIR imaging, opening new possibilities for whole-brain real-time functional imaging in rodents.http://www.sciencedirect.com/science/article/pii/S2213597924000880Calcium indicatorsIn vivo imagingBrainNear-infraredFluorescenceBiliverdin |
| spellingShingle | Sarah F. Shaykevich Justin P. Little Yong Qian Marie-Eve Paquet Robert E. Campbell Daniel Razansky Shy Shoham Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G Photoacoustics Calcium indicators In vivo imaging Brain Near-infrared Fluorescence Biliverdin |
| title | Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G |
| title_full | Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G |
| title_fullStr | Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G |
| title_full_unstemmed | Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G |
| title_short | Multimodal fluorescence-optoacoustic in vivo imaging of the near-infrared calcium ion indicator NIR-GECO2G |
| title_sort | multimodal fluorescence optoacoustic in vivo imaging of the near infrared calcium ion indicator nir geco2g |
| topic | Calcium indicators In vivo imaging Brain Near-infrared Fluorescence Biliverdin |
| url | http://www.sciencedirect.com/science/article/pii/S2213597924000880 |
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