A Hydrogen Peroxide Responsive Biotin-Guided Near-Infrared Hemicyanine-Based Fluorescent Probe for Early Cancer Diagnosis
H<sub>2</sub>O<sub>2</sub> plays an important role in oxidative damage and redox signaling. Studies have shown that abnormal levels of H<sub>2</sub>O<sub>2</sub> are closely related to the development of cancer. The levels of H<sub>2</sub>O...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/13/3/104 |
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| Summary: | H<sub>2</sub>O<sub>2</sub> plays an important role in oxidative damage and redox signaling. Studies have shown that abnormal levels of H<sub>2</sub>O<sub>2</sub> are closely related to the development of cancer. The levels of H<sub>2</sub>O<sub>2</sub> in tumor cells are higher than in normal cells. Thus, it is of great importance to develop a fluorescent probe to monitor the level of H<sub>2</sub>O<sub>2</sub> in vivo. This work reports a new biotin-guided NIR fluorescent probe, <b>Bio-B-Cy</b>, consisting of boronic acid ester as a H<sub>2</sub>O<sub>2</sub>-recognition site and biotin as a tumor binding site, which accelerates the fluorescence response to H<sub>2</sub>O<sub>2</sub> in vivo. <b>Bio-B-Cy</b> exhibits good sensitivity and selectivity toward H<sub>2</sub>O<sub>2</sub>. In addition, <b>Bio-B-Cy</b> shows a dose-dependent response to H<sub>2</sub>O<sub>2</sub> and the detection limit is 0.14 μM. We further demonstrate that <b>Bio-B-Cy</b> could successfully detect the H<sub>2</sub>O<sub>2</sub> in biotin receptor-positive cancer cells and tumor tissues. Based on the results, <b>Bio-B-Cy</b> has the potential to serve as an efficient tool for early diagnosis of cancer. |
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| ISSN: | 2227-9040 |