L-DOPA Uptake in Astrocytic Endfeet Enwrapping Blood Vessels in Rat Brain
Astrocyte endfeet surround brain blood vessels and can play a role in the delivery of therapeutic drugs for Parkinson’s disease. However, there is no previous evidence of the presence of LAT transporter for L-DOPA in brain astrocytes except in culture. Using systemic L-DOPA administration and a comb...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Parkinson's Disease |
| Online Access: | http://dx.doi.org/10.1155/2012/321406 |
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| author | M. Y. Inyushin A. Huertas Y. V. Kucheryavykh L. Y. Kucheryavykh V. Tsydzik P. Sanabria M. J. Eaton S. N. Skatchkov L. V. Rojas W. D. Wessinger |
| author_facet | M. Y. Inyushin A. Huertas Y. V. Kucheryavykh L. Y. Kucheryavykh V. Tsydzik P. Sanabria M. J. Eaton S. N. Skatchkov L. V. Rojas W. D. Wessinger |
| author_sort | M. Y. Inyushin |
| collection | DOAJ |
| description | Astrocyte endfeet surround brain blood vessels and can play a role in the delivery of therapeutic drugs for Parkinson’s disease. However, there is no previous evidence of the presence of LAT transporter for L-DOPA in brain astrocytes except in culture. Using systemic L-DOPA administration and a combination of patch clamp, histochemistry and confocal microscopy we found that L-DOPA is accumulated mainly in astrocyte cell bodies, astrocytic endfeet surrounding blood vessels, and pericytes. In brain slices: (1) astrocytes were exposed to ASP+, a fluorescent monoamine analog of MPP+; (2) ASP+ taken up by astrocytes was colocalized with L-DOPA fluorescence in (3) glial somata and in the endfeet attached to blood vessels; (4) these astrocytes have an electrogenic transporter current elicited by ASP+, but intriguingly not by L-DOPA, suggesting a different pathway for monoamines and L-DOPA via astrocytic membrane. (5) The pattern of monoamine oxidase (MAO type B) allocation in pericytes and astrocytic endfeet was similar to that of L-DOPA accumulation. We conclude that astrocytes control L-DOPA uptake and metabolism and, therefore, may play a key role in regulating brain dopamine level during dopamine-associated diseases. These data also suggest that different transporter mechanisms may exist for monoamines and L-DOPA. |
| format | Article |
| id | doaj-art-a5a8ff872c8045e0b655317c4e374d2a |
| institution | Kabale University |
| issn | 2090-8083 2042-0080 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Parkinson's Disease |
| spelling | doaj-art-a5a8ff872c8045e0b655317c4e374d2a2025-08-20T03:38:30ZengWileyParkinson's Disease2090-80832042-00802012-01-01201210.1155/2012/321406321406L-DOPA Uptake in Astrocytic Endfeet Enwrapping Blood Vessels in Rat BrainM. Y. Inyushin0A. Huertas1Y. V. Kucheryavykh2L. Y. Kucheryavykh3V. Tsydzik4P. Sanabria5M. J. Eaton6S. N. Skatchkov7L. V. Rojas8W. D. Wessinger9Department of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Physiology, Universidad Central del Caribe, Bayamón, PR 00956, USADepartment of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USAAstrocyte endfeet surround brain blood vessels and can play a role in the delivery of therapeutic drugs for Parkinson’s disease. However, there is no previous evidence of the presence of LAT transporter for L-DOPA in brain astrocytes except in culture. Using systemic L-DOPA administration and a combination of patch clamp, histochemistry and confocal microscopy we found that L-DOPA is accumulated mainly in astrocyte cell bodies, astrocytic endfeet surrounding blood vessels, and pericytes. In brain slices: (1) astrocytes were exposed to ASP+, a fluorescent monoamine analog of MPP+; (2) ASP+ taken up by astrocytes was colocalized with L-DOPA fluorescence in (3) glial somata and in the endfeet attached to blood vessels; (4) these astrocytes have an electrogenic transporter current elicited by ASP+, but intriguingly not by L-DOPA, suggesting a different pathway for monoamines and L-DOPA via astrocytic membrane. (5) The pattern of monoamine oxidase (MAO type B) allocation in pericytes and astrocytic endfeet was similar to that of L-DOPA accumulation. We conclude that astrocytes control L-DOPA uptake and metabolism and, therefore, may play a key role in regulating brain dopamine level during dopamine-associated diseases. These data also suggest that different transporter mechanisms may exist for monoamines and L-DOPA.http://dx.doi.org/10.1155/2012/321406 |
| spellingShingle | M. Y. Inyushin A. Huertas Y. V. Kucheryavykh L. Y. Kucheryavykh V. Tsydzik P. Sanabria M. J. Eaton S. N. Skatchkov L. V. Rojas W. D. Wessinger L-DOPA Uptake in Astrocytic Endfeet Enwrapping Blood Vessels in Rat Brain Parkinson's Disease |
| title | L-DOPA Uptake in Astrocytic Endfeet Enwrapping Blood Vessels in Rat Brain |
| title_full | L-DOPA Uptake in Astrocytic Endfeet Enwrapping Blood Vessels in Rat Brain |
| title_fullStr | L-DOPA Uptake in Astrocytic Endfeet Enwrapping Blood Vessels in Rat Brain |
| title_full_unstemmed | L-DOPA Uptake in Astrocytic Endfeet Enwrapping Blood Vessels in Rat Brain |
| title_short | L-DOPA Uptake in Astrocytic Endfeet Enwrapping Blood Vessels in Rat Brain |
| title_sort | l dopa uptake in astrocytic endfeet enwrapping blood vessels in rat brain |
| url | http://dx.doi.org/10.1155/2012/321406 |
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