Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitary
Abstract The pituitary gland is a key endocrine gland with various physiological functions including metabolism, growth, and reproduction. It comprises several distinct cell populations that release multiple polypeptide hormones. Although the major endocrine cell types are conserved across taxa, the...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04376-3 |
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| _version_ | 1841544992616611840 |
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| author | Liang Liu Jun Kitano Shuji Shigenobu Asano Ishikawa |
| author_facet | Liang Liu Jun Kitano Shuji Shigenobu Asano Ishikawa |
| author_sort | Liang Liu |
| collection | DOAJ |
| description | Abstract The pituitary gland is a key endocrine gland with various physiological functions including metabolism, growth, and reproduction. It comprises several distinct cell populations that release multiple polypeptide hormones. Although the major endocrine cell types are conserved across taxa, the regulatory mechanisms of gene expression and chromatin organization in specific cell types remain poorly understood. Here, we performed simultaneous profiling of the transcriptome and chromatin landscapes in the pituitary cells of the three-spined stickleback (Gasterosteus aculeatus), which represents a good model for investigating the genetic mechanisms underlying adaptive evolution. We obtained pairwise gene expression and chromatin profiles for 5184 cells under short- and long-day conditions. Using three independent clustering analyses, we identified 16 distinct cell clusters and validated their consistency. These results advance our understanding of the regulatory dynamics in the pituitary gland and provide a reference for future research on comparative physiology and evolutionary biology. |
| format | Article |
| id | doaj-art-a57f68576bd24861a0a8d87ab1b195ed |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-a57f68576bd24861a0a8d87ab1b195ed2025-01-12T12:07:56ZengNature PortfolioScientific Data2052-44632025-01-0112111510.1038/s41597-025-04376-3Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitaryLiang Liu0Jun Kitano1Shuji Shigenobu2Asano Ishikawa3Laboratory of Molecular Ecological Genetics, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of TokyoEcological Genetics Laboratory, National Institute of GeneticsLaboratory of Evolutionary Genomics, National Institute for Basic BiologyLaboratory of Molecular Ecological Genetics, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of TokyoAbstract The pituitary gland is a key endocrine gland with various physiological functions including metabolism, growth, and reproduction. It comprises several distinct cell populations that release multiple polypeptide hormones. Although the major endocrine cell types are conserved across taxa, the regulatory mechanisms of gene expression and chromatin organization in specific cell types remain poorly understood. Here, we performed simultaneous profiling of the transcriptome and chromatin landscapes in the pituitary cells of the three-spined stickleback (Gasterosteus aculeatus), which represents a good model for investigating the genetic mechanisms underlying adaptive evolution. We obtained pairwise gene expression and chromatin profiles for 5184 cells under short- and long-day conditions. Using three independent clustering analyses, we identified 16 distinct cell clusters and validated their consistency. These results advance our understanding of the regulatory dynamics in the pituitary gland and provide a reference for future research on comparative physiology and evolutionary biology.https://doi.org/10.1038/s41597-025-04376-3 |
| spellingShingle | Liang Liu Jun Kitano Shuji Shigenobu Asano Ishikawa Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitary Scientific Data |
| title | Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitary |
| title_full | Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitary |
| title_fullStr | Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitary |
| title_full_unstemmed | Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitary |
| title_short | Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitary |
| title_sort | co profiling of single cell gene expression and chromatin landscapes in stickleback pituitary |
| url | https://doi.org/10.1038/s41597-025-04376-3 |
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