Identification of Bioactive Compounds from the Roots of <i>Rehmannia glutinosa</i> and Their In Silico and In Vitro AMPK Activation Potential
<i>Rehmannia glutinosa</i> Libosch., which belongs to the Orobanchaceae family, is a perennial herb found in China, Japan, and Korea. In traditional medicine, it is used to cool the body, improve water metabolism in the kidney, and provide protection from metabolic diseases such as type...
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2024-12-01
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| author | Hwaryeong Lee Isoo Youn Sang Gyun Noh Hyun Woo Kim Eunhye Song Sang-Jip Nam Hae Young Chung Eun Kyoung Seo |
| author_facet | Hwaryeong Lee Isoo Youn Sang Gyun Noh Hyun Woo Kim Eunhye Song Sang-Jip Nam Hae Young Chung Eun Kyoung Seo |
| author_sort | Hwaryeong Lee |
| collection | DOAJ |
| description | <i>Rehmannia glutinosa</i> Libosch., which belongs to the Orobanchaceae family, is a perennial herb found in China, Japan, and Korea. In traditional medicine, it is used to cool the body, improve water metabolism in the kidney, and provide protection from metabolic diseases such as type 2 diabetes mellitus (T2DM) and obesity. In this study, three new compounds were isolated from the roots of <i>R. glutinosa</i>, along with eighteen known compounds. Structure elucidation was performed with spectroscopic analyses including nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopy. As the AMP-activated protein kinase (AMPK) signaling pathway is reportedly related to metabolic diseases, AMPK activation studies were conducted using in silico simulations and in vitro assays. Among the isolated compounds, <b>1</b> showed a potential as an AMPK activator in both in silico simulations and in vitro experiments. Our findings expand the chemical profiles of the plant <i>R. glutinosa</i> and suggest that one newly found compound (<b>1</b>) activates AMPK. |
| format | Article |
| id | doaj-art-a566050653de4fba838393ef58d64d38 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-a566050653de4fba838393ef58d64d382024-12-27T14:42:56ZengMDPI AGMolecules1420-30492024-12-012924600910.3390/molecules29246009Identification of Bioactive Compounds from the Roots of <i>Rehmannia glutinosa</i> and Their In Silico and In Vitro AMPK Activation PotentialHwaryeong Lee0Isoo Youn1Sang Gyun Noh2Hyun Woo Kim3Eunhye Song4Sang-Jip Nam5Hae Young Chung6Eun Kyoung Seo7Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of KoreaGraduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaGraduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of KoreaDepartment of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Republic of KoreaGraduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea<i>Rehmannia glutinosa</i> Libosch., which belongs to the Orobanchaceae family, is a perennial herb found in China, Japan, and Korea. In traditional medicine, it is used to cool the body, improve water metabolism in the kidney, and provide protection from metabolic diseases such as type 2 diabetes mellitus (T2DM) and obesity. In this study, three new compounds were isolated from the roots of <i>R. glutinosa</i>, along with eighteen known compounds. Structure elucidation was performed with spectroscopic analyses including nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopy. As the AMP-activated protein kinase (AMPK) signaling pathway is reportedly related to metabolic diseases, AMPK activation studies were conducted using in silico simulations and in vitro assays. Among the isolated compounds, <b>1</b> showed a potential as an AMPK activator in both in silico simulations and in vitro experiments. Our findings expand the chemical profiles of the plant <i>R. glutinosa</i> and suggest that one newly found compound (<b>1</b>) activates AMPK.https://www.mdpi.com/1420-3049/29/24/6009<i>Rehmannia glutinosa</i>uridine derivativeiridoid glycosidephenylpropanoid glycosidesAMP-activated protein kinase |
| spellingShingle | Hwaryeong Lee Isoo Youn Sang Gyun Noh Hyun Woo Kim Eunhye Song Sang-Jip Nam Hae Young Chung Eun Kyoung Seo Identification of Bioactive Compounds from the Roots of <i>Rehmannia glutinosa</i> and Their In Silico and In Vitro AMPK Activation Potential Molecules <i>Rehmannia glutinosa</i> uridine derivative iridoid glycoside phenylpropanoid glycosides AMP-activated protein kinase |
| title | Identification of Bioactive Compounds from the Roots of <i>Rehmannia glutinosa</i> and Their In Silico and In Vitro AMPK Activation Potential |
| title_full | Identification of Bioactive Compounds from the Roots of <i>Rehmannia glutinosa</i> and Their In Silico and In Vitro AMPK Activation Potential |
| title_fullStr | Identification of Bioactive Compounds from the Roots of <i>Rehmannia glutinosa</i> and Their In Silico and In Vitro AMPK Activation Potential |
| title_full_unstemmed | Identification of Bioactive Compounds from the Roots of <i>Rehmannia glutinosa</i> and Their In Silico and In Vitro AMPK Activation Potential |
| title_short | Identification of Bioactive Compounds from the Roots of <i>Rehmannia glutinosa</i> and Their In Silico and In Vitro AMPK Activation Potential |
| title_sort | identification of bioactive compounds from the roots of i rehmannia glutinosa i and their in silico and in vitro ampk activation potential |
| topic | <i>Rehmannia glutinosa</i> uridine derivative iridoid glycoside phenylpropanoid glycosides AMP-activated protein kinase |
| url | https://www.mdpi.com/1420-3049/29/24/6009 |
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