The Potential of Dehydrated <i>Geniotrigona thoracica</i> Stingless Bee Honey against Metabolic Syndrome in Rats Induced by a High-Carbohydrate, High-Fat Diet

Background/Objectives: Metabolic syndrome (MS) is diagnosed when at least three out of five key risk factors are present: obesity, high blood pressure, insulin resistance, high triglycerides (TG) and low high-density lipoprotein (HDL). MS is often associated with chronic low-grade inflammation. Rece...

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Main Authors: Liyana Nabihah Ikhsan, Kok-Yong Chin, Fairus Ahmad
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Pharmaceuticals
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Online Access:https://www.mdpi.com/1424-8247/17/11/1427
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author Liyana Nabihah Ikhsan
Kok-Yong Chin
Fairus Ahmad
author_facet Liyana Nabihah Ikhsan
Kok-Yong Chin
Fairus Ahmad
author_sort Liyana Nabihah Ikhsan
collection DOAJ
description Background/Objectives: Metabolic syndrome (MS) is diagnosed when at least three out of five key risk factors are present: obesity, high blood pressure, insulin resistance, high triglycerides (TG) and low high-density lipoprotein (HDL). MS is often associated with chronic low-grade inflammation. Recent studies have shown that raw stingless bee honey (SBH) can alleviate MS risk factors. However, the high moisture content in raw SBH predisposes it to fermentation, which can degrade its quality. Therefore, dehydrating SBH is necessary to prevent the fermentation process. This study aimed to compare the effects of dehydrated (DeGT) and raw (RGT) SBH from <i>Geniotrigona thoracica</i> species on high-carbohydrate, high-fat diet (HCHF)-induced MS in rats. Methods: Twenty-four male Wistar rats were divided into four groups: control (C), HCHF-induced MS without treatment (MS), HCHF-induced MS treated with DeGT (MS+DeGT) and HCHF-induced MS treated with RGT (MS+RGT). Group C received standard rat chow, while the other groups were fed with HCHF diet for 16 weeks. In the final eight weeks, two HCHF-induced groups received their respective SBH treatments. Results: Both DeGT and RGT treatments reduced energy intake, fat mass, high blood pressure, inflammatory (tumour necrosis factor-alpha (TNF-α)) and obesity (the leptin/adiponectin (L/A) ratio, corticosterone, 11 beta-hydroxysteroid dehydrogenase type-1 (11βHSD1)) markers, as well as prevented histomorphometry changes (prevented adipocyte hypertrophy, increased the Bowman’s space area and glomerular atrophy). Additionally, DeGT increased serum HDL levels, while RGT reduced serum TG, leptin and other inflammatory markers (interleukin-6 (IL-6) and interleukin-1 beta (IL-1β)), as well as hepatosteatosis. Conclusions: While DeGT demonstrates potential as a preventive agent for MS, RGT exhibited more pronounced anti-MS effects in this study.
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spelling doaj-art-a54dbc038ceb409a9dd86b014f22c9802024-11-26T18:17:07ZengMDPI AGPharmaceuticals1424-82472024-10-011711142710.3390/ph17111427The Potential of Dehydrated <i>Geniotrigona thoracica</i> Stingless Bee Honey against Metabolic Syndrome in Rats Induced by a High-Carbohydrate, High-Fat DietLiyana Nabihah Ikhsan0Kok-Yong Chin1Fairus Ahmad2Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaDepartment of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaBackground/Objectives: Metabolic syndrome (MS) is diagnosed when at least three out of five key risk factors are present: obesity, high blood pressure, insulin resistance, high triglycerides (TG) and low high-density lipoprotein (HDL). MS is often associated with chronic low-grade inflammation. Recent studies have shown that raw stingless bee honey (SBH) can alleviate MS risk factors. However, the high moisture content in raw SBH predisposes it to fermentation, which can degrade its quality. Therefore, dehydrating SBH is necessary to prevent the fermentation process. This study aimed to compare the effects of dehydrated (DeGT) and raw (RGT) SBH from <i>Geniotrigona thoracica</i> species on high-carbohydrate, high-fat diet (HCHF)-induced MS in rats. Methods: Twenty-four male Wistar rats were divided into four groups: control (C), HCHF-induced MS without treatment (MS), HCHF-induced MS treated with DeGT (MS+DeGT) and HCHF-induced MS treated with RGT (MS+RGT). Group C received standard rat chow, while the other groups were fed with HCHF diet for 16 weeks. In the final eight weeks, two HCHF-induced groups received their respective SBH treatments. Results: Both DeGT and RGT treatments reduced energy intake, fat mass, high blood pressure, inflammatory (tumour necrosis factor-alpha (TNF-α)) and obesity (the leptin/adiponectin (L/A) ratio, corticosterone, 11 beta-hydroxysteroid dehydrogenase type-1 (11βHSD1)) markers, as well as prevented histomorphometry changes (prevented adipocyte hypertrophy, increased the Bowman’s space area and glomerular atrophy). Additionally, DeGT increased serum HDL levels, while RGT reduced serum TG, leptin and other inflammatory markers (interleukin-6 (IL-6) and interleukin-1 beta (IL-1β)), as well as hepatosteatosis. Conclusions: While DeGT demonstrates potential as a preventive agent for MS, RGT exhibited more pronounced anti-MS effects in this study.https://www.mdpi.com/1424-8247/17/11/1427honeystingless bee honeyhoney dehydrationanti-inflammatoryobesity<i>Geniotrigona thoracica</i>
spellingShingle Liyana Nabihah Ikhsan
Kok-Yong Chin
Fairus Ahmad
The Potential of Dehydrated <i>Geniotrigona thoracica</i> Stingless Bee Honey against Metabolic Syndrome in Rats Induced by a High-Carbohydrate, High-Fat Diet
Pharmaceuticals
honey
stingless bee honey
honey dehydration
anti-inflammatory
obesity
<i>Geniotrigona thoracica</i>
title The Potential of Dehydrated <i>Geniotrigona thoracica</i> Stingless Bee Honey against Metabolic Syndrome in Rats Induced by a High-Carbohydrate, High-Fat Diet
title_full The Potential of Dehydrated <i>Geniotrigona thoracica</i> Stingless Bee Honey against Metabolic Syndrome in Rats Induced by a High-Carbohydrate, High-Fat Diet
title_fullStr The Potential of Dehydrated <i>Geniotrigona thoracica</i> Stingless Bee Honey against Metabolic Syndrome in Rats Induced by a High-Carbohydrate, High-Fat Diet
title_full_unstemmed The Potential of Dehydrated <i>Geniotrigona thoracica</i> Stingless Bee Honey against Metabolic Syndrome in Rats Induced by a High-Carbohydrate, High-Fat Diet
title_short The Potential of Dehydrated <i>Geniotrigona thoracica</i> Stingless Bee Honey against Metabolic Syndrome in Rats Induced by a High-Carbohydrate, High-Fat Diet
title_sort potential of dehydrated i geniotrigona thoracica i stingless bee honey against metabolic syndrome in rats induced by a high carbohydrate high fat diet
topic honey
stingless bee honey
honey dehydration
anti-inflammatory
obesity
<i>Geniotrigona thoracica</i>
url https://www.mdpi.com/1424-8247/17/11/1427
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