Urinary presepsin is a novel biomarker capable of directly assessing monocyte/macrophage infiltration in kidney diseases

Abstract Serum presepsin levels are elevated during sepsis and are widely employed in clinical practice. However, the association between urinary presepsin and kidney diseases remains elusive. Given that monocytes/macrophages, primary presepsin producers, are closely associated with the pathophysiol...

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Main Authors: Shunsuke Niwa, Akihito Tanaka, Kazuhiro Furuhashi, Keita Hattori, Chikao Onogi, Keisuke Sunohara, Akiko Owaki, Akihisa Kato, Tomohiro Kawazoe, Yu Watanabe, Eri Koshi-Ito, Noritoshi Kato, Tomoki Kosugi, Shoichi Maruyama
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Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-80686-7
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author Shunsuke Niwa
Akihito Tanaka
Kazuhiro Furuhashi
Keita Hattori
Chikao Onogi
Keisuke Sunohara
Akiko Owaki
Akihisa Kato
Tomohiro Kawazoe
Yu Watanabe
Eri Koshi-Ito
Noritoshi Kato
Tomoki Kosugi
Shoichi Maruyama
author_facet Shunsuke Niwa
Akihito Tanaka
Kazuhiro Furuhashi
Keita Hattori
Chikao Onogi
Keisuke Sunohara
Akiko Owaki
Akihisa Kato
Tomohiro Kawazoe
Yu Watanabe
Eri Koshi-Ito
Noritoshi Kato
Tomoki Kosugi
Shoichi Maruyama
author_sort Shunsuke Niwa
collection DOAJ
description Abstract Serum presepsin levels are elevated during sepsis and are widely employed in clinical practice. However, the association between urinary presepsin and kidney diseases remains elusive. Given that monocytes/macrophages, primary presepsin producers, are closely associated with the pathophysiology of nephritis, we explored the potential of urinary presepsin as a kidney disease biomarker. In a cross-sectional study involving patients who underwent kidney biopsy (n = 463 patients; 43% female, median age 58 years), the median urinary presepsin/creatinine levels were 590 (interquartile range [IQR], 244–1276), 1023 (IQR, 491–2749), 1429 (IQR, 644–2725), and 3518 (IQR, 2084–6321) ng/g creatinine, indicating minimal (< 5%), mild (5–25%), moderate (26–50%), and severe (> 50%) interstitial inflammatory cell infiltration in biopsy samples, respectively. The area under the curve of urinary presepsin/creatinine (0.81) had a higher accuracy for distinguishing severe interstitial inflammatory cell infiltration than that of the N-acetyl-β-D-glucosaminidase/creatinine (0.70) (P = 0.003). The tubulointerstitial nephritis group had the highest urinary presepsin/creatinine level. Immunofluorescence staining revealed that monocytes and macrophages predominantly expressed presepsin in the kidney interstitium, with the stained area positively and significantly correlated with presepsin/creatinine values (r = 0.57, P = 0.02). Urinary presepsin could be a biomarker for directly assessing monocyte/macrophage infiltration in kidney disease.
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spelling doaj-art-a540e64eef3f421ea84f3920c54dfdba2024-12-08T12:26:48ZengNature PortfolioScientific Reports2045-23222024-12-0114111210.1038/s41598-024-80686-7Urinary presepsin is a novel biomarker capable of directly assessing monocyte/macrophage infiltration in kidney diseasesShunsuke Niwa0Akihito Tanaka1Kazuhiro Furuhashi2Keita Hattori3Chikao Onogi4Keisuke Sunohara5Akiko Owaki6Akihisa Kato7Tomohiro Kawazoe8Yu Watanabe9Eri Koshi-Ito10Noritoshi Kato11Tomoki Kosugi12Shoichi Maruyama13Department of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University HospitalDepartment of Nephrology, Nagoya University HospitalDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineDepartment of Nephrology, Nagoya University Graduate School of MedicineAbstract Serum presepsin levels are elevated during sepsis and are widely employed in clinical practice. However, the association between urinary presepsin and kidney diseases remains elusive. Given that monocytes/macrophages, primary presepsin producers, are closely associated with the pathophysiology of nephritis, we explored the potential of urinary presepsin as a kidney disease biomarker. In a cross-sectional study involving patients who underwent kidney biopsy (n = 463 patients; 43% female, median age 58 years), the median urinary presepsin/creatinine levels were 590 (interquartile range [IQR], 244–1276), 1023 (IQR, 491–2749), 1429 (IQR, 644–2725), and 3518 (IQR, 2084–6321) ng/g creatinine, indicating minimal (< 5%), mild (5–25%), moderate (26–50%), and severe (> 50%) interstitial inflammatory cell infiltration in biopsy samples, respectively. The area under the curve of urinary presepsin/creatinine (0.81) had a higher accuracy for distinguishing severe interstitial inflammatory cell infiltration than that of the N-acetyl-β-D-glucosaminidase/creatinine (0.70) (P = 0.003). The tubulointerstitial nephritis group had the highest urinary presepsin/creatinine level. Immunofluorescence staining revealed that monocytes and macrophages predominantly expressed presepsin in the kidney interstitium, with the stained area positively and significantly correlated with presepsin/creatinine values (r = 0.57, P = 0.02). Urinary presepsin could be a biomarker for directly assessing monocyte/macrophage infiltration in kidney disease.https://doi.org/10.1038/s41598-024-80686-7BiomarkersKidney diseasesPresepsinUrinary level
spellingShingle Shunsuke Niwa
Akihito Tanaka
Kazuhiro Furuhashi
Keita Hattori
Chikao Onogi
Keisuke Sunohara
Akiko Owaki
Akihisa Kato
Tomohiro Kawazoe
Yu Watanabe
Eri Koshi-Ito
Noritoshi Kato
Tomoki Kosugi
Shoichi Maruyama
Urinary presepsin is a novel biomarker capable of directly assessing monocyte/macrophage infiltration in kidney diseases
Scientific Reports
Biomarkers
Kidney diseases
Presepsin
Urinary level
title Urinary presepsin is a novel biomarker capable of directly assessing monocyte/macrophage infiltration in kidney diseases
title_full Urinary presepsin is a novel biomarker capable of directly assessing monocyte/macrophage infiltration in kidney diseases
title_fullStr Urinary presepsin is a novel biomarker capable of directly assessing monocyte/macrophage infiltration in kidney diseases
title_full_unstemmed Urinary presepsin is a novel biomarker capable of directly assessing monocyte/macrophage infiltration in kidney diseases
title_short Urinary presepsin is a novel biomarker capable of directly assessing monocyte/macrophage infiltration in kidney diseases
title_sort urinary presepsin is a novel biomarker capable of directly assessing monocyte macrophage infiltration in kidney diseases
topic Biomarkers
Kidney diseases
Presepsin
Urinary level
url https://doi.org/10.1038/s41598-024-80686-7
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